Abstract:
Epimastigotes from several Trypanosoma cruzi stocks were labeled by iodination with Chloramine T and their proteins detected by gel electrophoresis and autoradiography. The labeled proteins from the parasite surface were detected after immunoprecipitation with antisera against fixed trypanosomes or from infected rabbits. These antisera were able to recognize one or more proteins in all T. cruzi isolates analyzed, but the individual patterns differed from each other. Variations in the surface protein patterns were also observed in two Tulahuen stocks kept during several years under different conditions. Growth medium as well as the stage of growth at which the parasites were collected had also an effect upon the relative amount of the observed labeled proteins. © 1984 Martinus Nijhoff Publishers.
Registro:
Documento: |
Artículo
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Título: | Surface proteins in different isolates of Trypanosoma cruzi epimastigotes |
Autor: | Gonzalez, N.S.; Sanchez, D.O.; Frasch, A.C.C.; Algranati, I.D. |
Filiación: | Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', and Facultad de Ciencias Exactas y Naturales Obligado 2490, Buenos Aires, 1428, Argentina Catedra de Quimica Biologica, Facultad de Medicina, Paraguay 2155, 5 P, Buenos Aires, 1121, Argentina
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Palabras clave: | membrane protein; parasite antigen; animal; article; immunology; molecular weight; Trypanosoma cruzi; Animal; Antigens, Protozoan; Membrane Proteins; Molecular Weight; Support, Non-U.S. Gov't; Trypanosoma cruzi |
Año: | 1984
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Volumen: | 63
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Número: | 2
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Página de inicio: | 157
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Página de fin: | 164
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DOI: |
http://dx.doi.org/10.1007/BF00285224 |
Título revista: | Molecular and Cellular Biochemistry
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Título revista abreviado: | Mol Cell Biochem
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ISSN: | 03008177
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CODEN: | MCBIB
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CAS: | Antigens, Protozoan; Membrane Proteins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v63_n2_p157_Gonzalez |
Referencias:
- Borst, P., Cross, G.A.M., (1982) Cell, 29, pp. 291-303
- Brener, Z., (1980) Adv Parasitol, 18, pp. 247-292
- Colli, W., (1979) Tropical Dis Res Ser, 2, pp. 131-153
- Alves, M.J.M., Colli, W., (1975) FEBS Lett, 52, pp. 188-190
- Snary, D., Hudson, L., (1979) FEBS Lett, 100, pp. 166-170
- Nogueira, N., Unkeless, J., Cohn, Z., (1982) Proc Natl Acad Sci USA, 79, pp. 1259-1263
- Lanar, D., Manning, J., (1983) J Cell Biochem Sup, 7 A, p. 24
- Zingales, B., Andrews, N.W., Kuwajima, V.Y., Colli, W., (1982) Mol Biochem Parasitol, 6, pp. 111-124
- Araujo, F.G., Remington, J.S., (1981) J Immunol, 127, pp. 855-859
- Pizzi T: Inmunologia de la Enfermedad de Chagas. Universidad de Chile (ed), 1956. pp 38–45; Silva, L.H., Nussenzweig, V., (1953) Folha Clin Biol Sao Paulo, 20, pp. 191-197
- Gonzalez Cappa, S.M., Katzin, A.M., Anasco, N., Lajmanovich, S., (1981) Medicina (Buenos Aires), 41, pp. 549-555
- Gonzalez Cappa, S.M., Chiale, P., Del Prado, G.E., Katzin, A.M., De Martini, G.W., Isola, E.L.D., Abramo Orrego, L., Segura, E.L., (1980) Medicina (Buenos Aires), 40, pp. 63-68
- Gerez de Burgos, N.M., Burgos, C., Blanco, A., Paulone, L., Segura, E.L., (1976) Acta Physiol Latinoam, 26, pp. 10-19
- Warren, L., (1960) J Parasitol, 46, pp. 529-538
- Laemmli, U.K., (1970) Nature, 227, pp. 680-685
- Avrameas, S., Ternynck, T., (1969) Immunochemistry, 6, pp. 53-66
- Segura, E.L., Cura, E.N., Paulone, I., Vazquez, C., Cerisola, J.A., (1974) J Protozool, 21, pp. 571-574
- Frasch, A.C.C., Segura, E.L., Cazzulo, J.J., Stoppani, A.O.M., (1978) Comp Biochem Physiol, 60 B, pp. 271-275
- Bordier, C., Michael Garavito, R., Armbruster, B., (1982) J Protozool, 29, pp. 560-565
- Plessmann Camargo, E., Barbieri, C.L., Jankevicius, J.V., Possible artifacts in the radioiodination of surface proteins of trypanosomatids (1982) Journal of Immunological Methods, 52, pp. 245-253
- Yoshida, N., (1983) Infect Immun, 40, pp. 836-839
- Snary, D., Ferguson, M.A.J., Scott, M.T., Allen, A.K., (1981) Mol Biochem Parasitol, 3, pp. 343-356
- Katzin AM, Colli W: VIII Annual Meeting on Basic Research on Chaga's Disease, Caxambu, Brazil, Abstract 193, 1981; Crane, M.J., Dvorak, J.A., (1982) Mol Biochem Parasitol, 5, pp. 333-341
- Enriquez, D., Piras, R., Piras, M.M., (1981) Mol Biochem Parasitol, 2, pp. 359-366
Citas:
---------- APA ----------
Gonzalez, N.S., Sanchez, D.O., Frasch, A.C.C. & Algranati, I.D.
(1984)
. Surface proteins in different isolates of Trypanosoma cruzi epimastigotes. Molecular and Cellular Biochemistry, 63(2), 157-164.
http://dx.doi.org/10.1007/BF00285224---------- CHICAGO ----------
Gonzalez, N.S., Sanchez, D.O., Frasch, A.C.C., Algranati, I.D.
"Surface proteins in different isolates of Trypanosoma cruzi epimastigotes"
. Molecular and Cellular Biochemistry 63, no. 2
(1984) : 157-164.
http://dx.doi.org/10.1007/BF00285224---------- MLA ----------
Gonzalez, N.S., Sanchez, D.O., Frasch, A.C.C., Algranati, I.D.
"Surface proteins in different isolates of Trypanosoma cruzi epimastigotes"
. Molecular and Cellular Biochemistry, vol. 63, no. 2, 1984, pp. 157-164.
http://dx.doi.org/10.1007/BF00285224---------- VANCOUVER ----------
Gonzalez, N.S., Sanchez, D.O., Frasch, A.C.C., Algranati, I.D. Surface proteins in different isolates of Trypanosoma cruzi epimastigotes. Mol Cell Biochem. 1984;63(2):157-164.
http://dx.doi.org/10.1007/BF00285224