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Abstract:

Ustilago maydis was surveyed for cyclic AMP-dependent protein kinase activity. Using a combination of ion-exchange and molecular filtration techniques, we demonstrate that there is only one form of cyclic AMP-dependent protein kinase in the cytosolic fraction of the fungus. The kinase activity is specifically activated by cyclic AMP and utilizes protamine and kemptide as substrates. Most, if not all, of the cyclic AMP binding detected in the soluble fraction is associated with the protein kinase activity. Cyclic AMP-dependent protein kinase is completely dissociated by cyclic AMP into catalytic and regulatory subunits having an apparent molecular weight of 35 000 daltons as judged by sucrose gradient centrifugation. © 1984 Martinus Nijhoff Publishers.

Registro:

Documento: Artículo
Título:Cyclic AMP-dependent protein kinase from Ustilago maydis
Autor:Kerner, N.; Passeron, S.
Filiación:Programa de Regulacion Hormonal y Metabolica, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:cyclic AMP; protein kinase; article; Basidiomycetes; comparative study; density gradient centrifugation; drug effect; enzymology; isolation and purification; metabolism; molecular weight; protein conformation; Ustilago; Basidiomycota; Centrifugation, Density Gradient; Comparative Study; Cyclic AMP; Molecular Weight; Protein Conformation; Protein Kinases; Support, Non-U.S. Gov't; Ustilago
Año:1984
Volumen:60
Número:2
Página de inicio:115
Página de fin:122
DOI: http://dx.doi.org/10.1007/BF00222481
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:cyclic AMP, 60-92-4; protein kinase, 9026-43-1; Cyclic AMP, 60-92-4; Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v60_n2_p115_Kerner

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Citas:

---------- APA ----------
Kerner, N. & Passeron, S. (1984) . Cyclic AMP-dependent protein kinase from Ustilago maydis. Molecular and Cellular Biochemistry, 60(2), 115-122.
http://dx.doi.org/10.1007/BF00222481
---------- CHICAGO ----------
Kerner, N., Passeron, S. "Cyclic AMP-dependent protein kinase from Ustilago maydis" . Molecular and Cellular Biochemistry 60, no. 2 (1984) : 115-122.
http://dx.doi.org/10.1007/BF00222481
---------- MLA ----------
Kerner, N., Passeron, S. "Cyclic AMP-dependent protein kinase from Ustilago maydis" . Molecular and Cellular Biochemistry, vol. 60, no. 2, 1984, pp. 115-122.
http://dx.doi.org/10.1007/BF00222481
---------- VANCOUVER ----------
Kerner, N., Passeron, S. Cyclic AMP-dependent protein kinase from Ustilago maydis. Mol Cell Biochem. 1984;60(2):115-122.
http://dx.doi.org/10.1007/BF00222481