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Abstract:

Cyclic AMP binding to Mucor rouxii protein kinase holoenzyme and free regulatory subunit was measured by the classical membrane filtration technique and by equilibrium dialysis. The results obtained demonstrate that the filtration method can be used without loss of any cyclic AMP binding site. Both methods unambiguously demonstrate that the number of molecules of cyclic AMP bound to the holoenzyme are half of those bound to the regulatory subunit. This result suggests that unshielding of new cyclic AMP binding sites occurs upon dissociation of the ternary complex holoenzyme-cyclic AMP. © 1983 Martinus Nijhoff Publishers.

Registro:

Documento: Artículo
Título:Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP
Autor:Moreno, S.; Pastori, R.; Passeron, S.
Filiación:Programa de Regulación Hormonal y Metabólica, Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:cyclic AMP; histone; protein kinase; article; binding site; density gradient centrifugation; dialysis; enzymology; filtration; macromolecule; metabolism; methodology; Mucor; Binding Sites; Centrifugation, Density Gradient; Cyclic AMP; Dialysis; Filtration; Histones; Macromolecular Systems; Mucor; Protein Kinases; Support, Non-U.S. Gov't
Año:1983
Volumen:52
Número:1
Página de inicio:13
Página de fin:16
DOI: http://dx.doi.org/10.1007/BF00230584
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:cyclic AMP, 60-92-4; histone, 9062-68-4; protein kinase, 9026-43-1; Cyclic AMP, 60-92-4; Histones; Macromolecular Systems; Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v52_n1_p13_Moreno

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Citas:

---------- APA ----------
Moreno, S., Pastori, R. & Passeron, S. (1983) . Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP. Molecular and Cellular Biochemistry, 52(1), 13-16.
http://dx.doi.org/10.1007/BF00230584
---------- CHICAGO ----------
Moreno, S., Pastori, R., Passeron, S. "Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP" . Molecular and Cellular Biochemistry 52, no. 1 (1983) : 13-16.
http://dx.doi.org/10.1007/BF00230584
---------- MLA ----------
Moreno, S., Pastori, R., Passeron, S. "Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP" . Molecular and Cellular Biochemistry, vol. 52, no. 1, 1983, pp. 13-16.
http://dx.doi.org/10.1007/BF00230584
---------- VANCOUVER ----------
Moreno, S., Pastori, R., Passeron, S. Protein kinase from Mucor rouxii - Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP. Mol Cell Biochem. 1983;52(1):13-16.
http://dx.doi.org/10.1007/BF00230584