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Bernard, E.A.; Guaragna, R.; Amaral, B.B.; Perry, M.L.S.; Pereira, I.R.G.; Ielpi, L.; Couso, R.O. "Formation of lipid-linked sugars in mycelial and yeast-like forms of Mucor rouxii" (1982) Molecular and Cellular Biochemistry. 45(1):41-48
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Abstract:

Cell wall fragments from both yeast-like and mycelial forms of the dimorphic fungus Mucor rouxii were used as enzymatic preparations to study the synthesis and role of prenyl-phospho-sugars in these systems. In the presence of GDP ['4C] mannose two main products were formed. One of them was characterized as dolichol-monophosphate β-mannose on the following basis: solubility in organic solvents, behaviour upon paper chromatography, DEAF cellulose column chromatography, mild acid hydrolysis, alkali treatment, catalytic reduction and phenol degradation. The other product was identified as a glicoprotein containing a single mannose unit linked to a serine or threonine residue. It was degraded with pronase and by mild NaOH-NaBH4 treatment all the radioactivity was released as free mannitol. When UDP[4C] glucose was employed as sugar donor two butanol soluble components were isolated. One of them (25%) was characterized as dolichol-monophosphate-ß-glucose on the basis of the same criteria as described above. The other one (75%) was neutral and was not studied in detail. Mycelial enzymes were about 40 times more active in the synthesis of the dolichol derivatives. In addition, large amounts of glycogen were detected. The role that both dolichol derivatives might play in glycoprotein biosynthesis is discussed. © 1982 Martinus Nijhoff/Dr W. Junk Publishers.

Registro:

Documento: Artículo
Título:Formation of lipid-linked sugars in mycelial and yeast-like forms of Mucor rouxii
Autor:Bernard, E.A.; Guaragna, R.; Amaral, B.B.; Perry, M.L.S.; Pereira, I.R.G.; Ielpi, L.; Couso, R.O.
Filiación:Departamento de Bioquimica, Instituto de Biociéncias UFRGS, Porto Alegre, 90000, Brazil
Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', Buenos Aires, 1428, Argentina
Palabras clave:glucose; lipid; mannose; polysaccharide; article; biosynthesis; cell wall; chemistry; metabolism; Mucor; Cell Wall; Chemistry; Glucose; Lipids; Mannose; Mucor; Polysaccharides; Support, Non-U.S. Gov't
Año:1982
Volumen:45
Número:1
Página de inicio:41
Página de fin:48
DOI: http://dx.doi.org/10.1007/BF01283162
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:glucose, 50-99-7, 84778-64-3; lipid, 66455-18-3; mannose, 31103-86-3, 3458-28-4; Glucose, 50-99-7; Lipids; Mannose, 31103-86-3; Polysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v45_n1_p41_Bernard

Referencias:

  • Lennarz, W.J., Scher, M.G., (1972) Biochim. Biophys. Acta, 265, pp. 417-441
  • Staneloni, R.J., Leloir, L.F., (1979) Trends Biochem. Sci., 4, pp. 65-67
  • Letoublon, R.C.P., Comte, J., Got, R., (1973) Eur. J. Biochem., 40, pp. 95-101
  • Gold, M.H., Hahn, H.J., (1976) Biochemistry, 15, pp. 1808-1814
  • Couso, R.O., Ielpi, L., Garcia, R.C., Dankert, M., (1980) Arch. Biochem. Biophys., 204, pp. 434-443
  • Garcia, R.C., Recondo, E., Dankert, M., (1974) Eur. J. Biochem., 43, pp. 93-105
  • Romero, P., Garcia, R.C., Dankert, M., (1977) Mol. Cell. Biochem., 16, pp. 205-212
  • Pont Lezica, R., Romero, P.A., Dankert, M.A., (1976) Plant Physiol., 58, pp. 675-680
  • Bartnicki-Garcia, S., Nickerson, W.J., (1962) J. Bacteriol., 84, pp. 829-840
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275
  • Arima, T., Spiro, M.J., Spiro, R.G., (1972) J. Biol. Chem., 247, pp. 1825-1835
  • Dankert, M., Wright, A., Kelly, W.S., Robbins, P.W., (1966) Arch. Biochem. Biophys., 116, pp. 425-435
  • Trevelyan, W.E., Procter, D.P., Harrison, J.S., (1950) Nature (Lond.), 166, pp. 444-445
  • Herscovics, A., Warren, C.D., Jeanloz, R.W., Wedgwood, J.F., Liu, I.Y., Strominger, J.L., (1974) FEBS Lett., 45, pp. 312-317
  • Pont Lezica, R., Brett, C.T., Romero Martinez, P., Dankert, M.A., (1975) Biochem. Biophys. Res. Commun., 66, pp. 980-987
  • Morrison, W.R., (1969) Biochim. Biophys. Acta, 176, pp. 537-546
  • Krisman, C.R., (1962) Analyt. Biochem., 4, pp. 17-23
  • Dow, J.M., Rubery, P.H., (1977) J. Gen. Microbiol., 99, pp. 29-41
  • Jung, P., Tanner, W., Identification of the Lipid Intermediate in Yeast Mannan Biosynthesis (1973) European Journal of Biochemistry, 37, pp. 1-6
  • Gibson, R., Kornfeld, S., Schlesinger, S., A role for oligosaccharides in glycoprotein biosynthesis (1980) Trends in Biochemical Sciences, 5, pp. 290-293

Citas:

---------- APA ----------
Bernard, E.A., Guaragna, R., Amaral, B.B., Perry, M.L.S., Pereira, I.R.G., Ielpi, L. & Couso, R.O. (1982) . Formation of lipid-linked sugars in mycelial and yeast-like forms of Mucor rouxii. Molecular and Cellular Biochemistry, 45(1), 41-48.
http://dx.doi.org/10.1007/BF01283162
---------- CHICAGO ----------
Bernard, E.A., Guaragna, R., Amaral, B.B., Perry, M.L.S., Pereira, I.R.G., Ielpi, L., et al. "Formation of lipid-linked sugars in mycelial and yeast-like forms of Mucor rouxii" . Molecular and Cellular Biochemistry 45, no. 1 (1982) : 41-48.
http://dx.doi.org/10.1007/BF01283162
---------- MLA ----------
Bernard, E.A., Guaragna, R., Amaral, B.B., Perry, M.L.S., Pereira, I.R.G., Ielpi, L., et al. "Formation of lipid-linked sugars in mycelial and yeast-like forms of Mucor rouxii" . Molecular and Cellular Biochemistry, vol. 45, no. 1, 1982, pp. 41-48.
http://dx.doi.org/10.1007/BF01283162
---------- VANCOUVER ----------
Bernard, E.A., Guaragna, R., Amaral, B.B., Perry, M.L.S., Pereira, I.R.G., Ielpi, L., et al. Formation of lipid-linked sugars in mycelial and yeast-like forms of Mucor rouxii. Mol Cell Biochem. 1982;45(1):41-48.
http://dx.doi.org/10.1007/BF01283162