Artículo

Gabrielli, M.; Romero, D.G.; Martini, C.N.; Raiger Iustman, L.J.; Vila, M.D.C. "MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes" (2018) Molecular and Cellular Biochemistry. 448(1-2):299-309
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Abstract:

We investigated for the first time the expression of melanoma cell adhesion molecule (MCAM) and its involvement in the differentiation of 3T3-L1 fibroblasts to adipocytes. We found that MCAM mRNA increased subsequent to the activation of the master regulator of adipogenesis, PPARγ, and this increase was maintained in the mature adipocytes. On the other hand, MCAM knockdown impaired differentiation and induction of PPARγ as well as expression of genes activated by PPARγ. However, events that precede and are necessary for early PPARγ activation, such as C/EBPβ induction, β-catenin downregulation, and ERK activation, were not affected in the MCAM knockdown cells. In keeping with this, the increase in PPARγ mRNA that precedes MCAM induction was not altered in the knockdown cells. In conclusion, our findings suggest that MCAM is a gene upregulated and involved in maintaining PPARγ induction in the late but not in the early stages of 3T3-L1 fibroblasts adipogenesis. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

Registro:

Documento: Artículo
Título:MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes
Autor:Gabrielli, M.; Romero, D.G.; Martini, C.N.; Raiger Iustman, L.J.; Vila, M.D.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Universidad de Buenos Aires, CONICET, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina
Department of Cell and Molecular Biology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, United States
Palabras clave:3T3-L1 fibroblasts; Adipogenesis; C/EBP beta; MCAM/CD146; PPAR gamma; adiponectin; beta catenin; CCAAT enhancer binding protein beta; CD146 antigen; messenger RNA; mitogen activated protein kinase; perilipin 1; peroxisome proliferator activated receptor gamma; CD146 antigen; Mcam protein, mouse; peroxisome proliferator activated receptor gamma; 3T3-L1 cell line; adipocyte; adipogenesis; animal cell; Article; cell differentiation; cell maturation; controlled study; down regulation; enzyme activation; gene expression; gene knockdown; immunofluorescence microscopy; mouse; mRNA expression level; NIH 3T3 cell line; nonhuman; protein expression level; protein function; quantitative analysis; real time polymerase chain reaction; upregulation; Western blotting; 3T3-L1 cell line; adipocyte; adipogenesis; animal; biosynthesis; cytology; fibroblast; gene expression regulation; gene knockdown; genetics; metabolism; 3T3-L1 Cells; Adipocytes; Adipogenesis; Animals; CD146 Antigen; Cell Differentiation; Fibroblasts; Gene Expression Regulation; Gene Knockdown Techniques; Mice; PPAR gamma
Año:2018
Volumen:448
Número:1-2
Página de inicio:299
Página de fin:309
DOI: http://dx.doi.org/10.1007/s11010-018-3334-8
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol. Cell. Biochem.
ISSN:03008177
CODEN:MCBIB
CAS:adiponectin, 283182-39-8; mitogen activated protein kinase, 142243-02-5; CD146 Antigen; Mcam protein, mouse; PPAR gamma
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v448_n1-2_p299_Gabrielli

Referencias:

  • Lean, M.E., Pathophysiology of obesity (2000) Proc Nutr Soc, 59, pp. 331-336. , COI: 1:STN:280:DC%2BD3cvksFyksQ%3D%3D
  • Rosen, E.D., Spiegelman, B.M., What we talk about when we talk about fat (2014) Cell, 156, pp. 20-44
  • Lauvrud, A.T., Kelk, P., Wiberg, M., Kingham, P.J., Characterization of human adipose tissue-derived stem cells with enhanced angiogenic and adipogenic properties (2017) J Tissue Eng Regen Med, 11, pp. 2490-2502
  • Farmer, S.R., Transcriptional control of adipocyte formation (2006) Cell Metab, 4, pp. 263-273
  • Gurnell, M., Wentworth, J.M., Agostini, M., Adams, M., Collingwood, T.N., Provenzano, C., Browne, P.O., Chatterjee, V.K., A dominant-negative peroxisome proliferator-activated receptor gamma (PPAR gamma) mutant is a constitutive repressor and inhibits PPARγ-mediated adipogenesis (2000) J Biol Chem, 275, pp. 5754-5759. , COI: 1:CAS:528:DC%2BD3cXhsFKktLc%3D
  • Tontonoz, P., Spiegelman, B.M., Fat and beyond: the diverse biology of PPARγ (2008) Annu Rev Biochem, 77, pp. 289-312
  • Gabrielli, M., Martini, C.N., Brandani, J.N., Iustman, L.J., Romero, D.G., Vila, M., del, C., Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation (2014) Dev Growth Differ, 56, pp. 143-151
  • Lehmann, J.M., Holzmann, B., Breitbart, E.W., Schmiegelow, P., Riethmüller, G., Johnson, J.P., Discrimination between benign and malignant cells of melanocytic lineage by two novel antigens, a glycoprotein with a molecular weight of 113,000 and a protein with a molecular weight of 76,000 (1987) Cancer Res, 47, pp. 841-845. , COI: 1:CAS:528:DyaL2sXhtFOksbg%3D, PID: 3542195
  • Wang, Z., Yan, X., CD146, a multi-functional molecule beyond adhesion (2013) Cancer Lett, 330, pp. 150-162
  • Russell, K.C., Phinney, D.G., Lacey, M.R., Barrilleaux, B.L., Meyertholen, K.E., O’Connor, K.C., In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment (2010) Stem Cells, 28, pp. 788-798
  • Flanagan, K., Fitzgerald, K., Baker, J., Regnstrom, K., Gardai, S., Bard, F., Mocci, S., Yednock, T., Laminin-411 is a vascular ligand for MCAM and facilitates TH17 cell entry into the CNS (2012) PLoS ONE, 7
  • Tu, T., Zhang, C., Yan, H., Luo, Y., Kong, R., Wen, P., Ye, Z., Yan, X., CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development (2015) Cell Res, 25, pp. 275-287
  • Ye, Z., Zhang, C., Tu, T., Sun, M., Liu, D., Lu, D., Feng, J., Yan, X., Wnt5a uses CD146 as a receptor to regulate cell motility and convergent extension (2013) Nat Commun, 4, p. 2803
  • Jiang, T., Zhuang, J., Duan, H., Luo, Y., Zeng, Q., Fan, K., Yan, H., Yan, X., CD146 is a coreceptor for VEGFR-2 in tumor angiogenesis (2012) Blood, 120, pp. 2330-2339
  • Anfosso, F., Bardin, N., Vivier, E., Sabatier, F., Sampol, J., Dignat-George, F., Outside-in signaling pathway linked to CD146 engagement in human endothelial cells (2001) J Biol Chem, 276, pp. 1564-1569
  • Moffat, J., Grueneberg, D.A., Yang, X., Kim, S.Y., Kloepfer, A.M., Hinkle, G., Piqani, B., Root, D.E., A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen (2006) Cell, 124, pp. 1283-1298
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685. , COI: 1:CAS:528:DC%2BD3MXlsFags7s%3D
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254. , COI: 1:CAS:528:DyaE28XksVehtrY%3D
  • Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Cardona, A., Fiji: an open-source platform for biological-image analysis (2012) Nat Methods, 9, pp. 676-682
  • Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B.C., Remm, M., Rozen, S.G., Primer3—new capabilities and interfaces (2012) Nucleic Acids Res, 40
  • Koh, Y.K., Lee, M.Y., Kim, J.W., Kim, M., Moon, J.S., Lee, Y.J., Ahn, Y.H., Kim, K.S., Lipin1 is a key factor for the maturation and maintenance of adipocytes in the regulatory network with CCAAT/enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma 2 (2008) J Biol Chem, 283, pp. 34896-34906
  • Toneatto, J., Guber, S., Charó, N.L., Susperreguy, S., Schwartz, J., Galigniana, M.D., Piwien-Pilipuk, G., Dynamic mitochondrial-nuclear redistribution of the immunophilin FKBP51 is regulated by the PKA signaling pathway to control gene expression during adipocyte differentiation (2013) J Cell Sci, 126, pp. 5357-5368
  • Zhang, L., Paddon, C., Lewis, M.D., Grennan-Jones, F., Ludgate, M., Gsalpha signalling suppresses PPARγ2 generation and inhibits 3T3L1 adipogenesis (2009) J Endocrinol, 202, pp. 207-215
  • Liu, J., DeYoung, S.M., Zhang, M., Zhang, M., Cheng, A., Saltiel, A.R., Changes in integrin expression during adipocyte differentiation (2005) Cell Metab, 2, pp. 165-177
  • Qiu, Z., Wei, Y., Chen, N., Jiang, M., Wu, J., Liao, K., DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes (2001) J Biol Chem, 276, pp. 11988-11995
  • Arimura, N., Horiba, T., Imagawa, M., Shimizu, M., Sato, R., The peroxisome proliferator-activated receptor gamma regulates expression of the Perilipin gene in adipocytes (2004) J Biol Chem, 279, pp. 10070-10076
  • Nagai, S., Shimizu, C., Umetsu, M., Taniguchi, S., Endo, M., Miyoshi, H., Yoshioka, N., Koike, T., Identification of a functional peroxisome proliferator-activated receptor responsive element within the murine Perilipin gene (2004) Endocrinology, 145, pp. 2346-2356
  • Cawthorn, W.P., Heyd, F., Hegyi, K., Sethi, J.K., Tumour necrosis factor-alpha inhibits adipogenesis via a beta-catenin/TCF4(TCF7L2)-dependent pathway (2007) Cell Death Differ, 14, pp. 1361-1373
  • Ross, S.E., Hemati, N., Longo, K.A., Bennett, C.N., Lucas, P.C., Erickson, R.L., MacDougald, O.A., Inhibition of adipogenesis by Wnt signaling (2000) Science, 289, pp. 950-953. , COI: 1:CAS:528:DC%2BD3cXlvFSmu7s%3D
  • Prusty, D., Park, B.H., Davis, K.E., Farmer, S.R., Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARγamma) and C/EBP alpha gene expression during the differentiation of 3T3-L1 preadipocytes (2002) J Biol Chem, 277, pp. 46226-46232
  • Greenberg, A.S., Coleman, R.A., Kraemer, F.B., McManaman, J.L., Obin, M.S., Puri, V., Yan, Q.W., Mashek, D.G., The role of lipid droplets in metabolic disease in rodents and humans (2011) J Clin Invest, 121, pp. 2102-2110
  • Tontonoz, P., Hu, E., Spiegelman, B.M., Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor (1994) Cell, 79, pp. 1147-1156. , COI: 1:STN:280:DyaK2M%2FpsVaqsg%3D%3D
  • Stopp, S., Bornhäuser, M., Ugarte, F., Wobus, M., Kuhn, M., Brenner, S., Thieme, S., Expression of the melanoma cell adhesion molecule in human mesenchymal stromal cells regulates proliferation, differentiation, and maintenance of hematopoietic stem and progenitor cells (2013) Haematologica, 98, pp. 505-513
  • Soccio, R.E., Chen, E.R., Lazar, M.A., Thiazolidinediones and the promise of insulin sensitization in type 2 diabetes (2014) Cell Metab, 20, pp. 573-591
  • Klöting, N., Fasshauer, M., Dietrich, A., Kovacs, P., Schön, M.R., Kern, M., Stumvoll, M., Blüher, M., Insulin-sensitive obesity (2010) Am J Physiol Endocrinol Metab, 299, pp. E506-E515
  • Bu, P., Gao, L., Zhuang, J., Feng, J., Yang, D., Yan, X., Anti-CD146 monoclonal antibody AA98 inhibits angiogenesis via suppression of nuclear factor-kappaB activation (2006) Mol Cancer Ther, 5, pp. 2872-2878
  • Zigler, M., Villares, G.J., Dobroff, A.S., Wang, H., Huang, L., Braeuer, R.R., Kamiya, T., Bar-Eli, M., Expression of Id-1 is regulated by MCAM/MUC18: a missing link in melanoma progression (2011) Cancer Res, 71, pp. 3494-3504
  • Niimi, T., Kumagai, C., Okano, M., Kitagaw, Y., Differentiation-dependent expression of laminin-8 (α4β1γ1) mRNAs in mouse 3T3-L1 adipocytes (1997) Matrix Biol, 16, pp. 223-230. , COI: 1:CAS:528:DyaK2sXnt1Cktrw%3D
  • Vaicik, M.K., Thyboll Kortesmaa, J., Movérare-Skrtic, S., Kortesmaa, J., Soininen, R., Bergström, G., Ohlsson, C., Tryggvason, K., Laminin α4 deficient mice exhibit decreased capacity for adipose tissue expansion and weight gain (2014) PLoS ONE, 10. , eCollection 2014
  • Mukherjee, R., Kim, S.W., Park, T., Choi, M.S., Yun, J.W., Targeted inhibition of galectin 1 by thiodigalactoside dramatically reduces body weight gain in diet-induced obese rats (2015) Int J Obes, 39, pp. 1349-1358
  • Mukherjee, R., Yun, J.W., Pharmacological inhibition of galectin-1 by lactulose alleviates weight gain in diet-induced obese rats (2016) Life Sci, 148, pp. 112-117

Citas:

---------- APA ----------
Gabrielli, M., Romero, D.G., Martini, C.N., Raiger Iustman, L.J. & Vila, M.D.C. (2018) . MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes. Molecular and Cellular Biochemistry, 448(1-2), 299-309.
http://dx.doi.org/10.1007/s11010-018-3334-8
---------- CHICAGO ----------
Gabrielli, M., Romero, D.G., Martini, C.N., Raiger Iustman, L.J., Vila, M.D.C. "MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes" . Molecular and Cellular Biochemistry 448, no. 1-2 (2018) : 299-309.
http://dx.doi.org/10.1007/s11010-018-3334-8
---------- MLA ----------
Gabrielli, M., Romero, D.G., Martini, C.N., Raiger Iustman, L.J., Vila, M.D.C. "MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes" . Molecular and Cellular Biochemistry, vol. 448, no. 1-2, 2018, pp. 299-309.
http://dx.doi.org/10.1007/s11010-018-3334-8
---------- VANCOUVER ----------
Gabrielli, M., Romero, D.G., Martini, C.N., Raiger Iustman, L.J., Vila, M.D.C. MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes. Mol. Cell. Biochem. 2018;448(1-2):299-309.
http://dx.doi.org/10.1007/s11010-018-3334-8