Artículo
Abstract:
Erythrocyte soluble phosphodiesterase activities show at least two interconvertible states: 'aggregated' and 'non-aggregated'. In the former state the existence of a high affinity component for cyclic AMP is favoured and the system is more active with cyclic AMP than with cyclic GMP as substrate. When the system is in the 'non-aggregated state', no activity with cyclic GMP is detected. Conversion to the 'non-aggregated state' is enhanced by dilution or by addition of magnesium ions. Upon isoelectric focusing of erythrocyte soluble extracts, phosphodiesterase activities can be resolved in two peaks: one specific for cyclic AMP located at pH 4.66 and the other specific for cyclic GMP focused at pH 4.95. Evidence would indicate that aggregation affects the enzyme specific for cyclic AMP. © 1982 Martinus Nijhoff/Dr W. Junk Publishers.
Registro:
| Documento: | Artículo |
| Título: | Cyclic nucleotide phosphodiesterase activities in rat erythrocytes |
| Autor: | Téllez-Iñón, M.T.; Torres, H.N. |
| Filiación: | Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', Obligado 2490, Buenos Aires, 1428, Argentina |
| Palabras clave: | cyclic amp; cyclic gmp; cyclic nucleotide phosphodiesterase; animal experiment; blood and hemopoietic system; erythrocyte; rat; 3',5'-Cyclic-GMP Phosphodiesterase; 3',5'-Cyclic-Nucleotide Phosphodiesterase; Animal; Chromatography, Gel; Erythrocytes; Isoelectric Focusing; Kinetics; Magnesium; Magnesium Chloride; Male; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't |
| Año: | 1982 |
| Volumen: | 43 |
| Número: | 3 |
| Página de inicio: | 161 |
| Página de fin: | 166 |
| DOI: | http://dx.doi.org/10.1007/BF00223007 |
| Título revista: | Molecular and Cellular Biochemistry |
| Título revista abreviado: | Mol Cell Biochem |
| ISSN: | 03008177 |
| CODEN: | MCBIB |
| CAS: | cyclic AMP, 60-92-4; cyclic GMP, 7665-99-8; cyclic nucleotide phosphodiesterase, 50812-31-2; 3',5'-Cyclic-GMP Phosphodiesterase, EC 3.1.4.35; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Magnesium Chloride, 7786-30-3; Magnesium, 7439-95-4 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v43_n3_p161_TellezInon |
Referencias:
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Citas:
---------- APA ----------
Téllez-Iñón, M.T. & Torres, H.N. (1982) . Cyclic nucleotide phosphodiesterase activities in rat erythrocytes. Molecular and Cellular Biochemistry, 43(3), 161-166.http://dx.doi.org/10.1007/BF00223007
---------- CHICAGO ----------
Téllez-Iñón, M.T., Torres, H.N. "Cyclic nucleotide phosphodiesterase activities in rat erythrocytes" . Molecular and Cellular Biochemistry 43, no. 3 (1982) : 161-166.http://dx.doi.org/10.1007/BF00223007
---------- MLA ----------
Téllez-Iñón, M.T., Torres, H.N. "Cyclic nucleotide phosphodiesterase activities in rat erythrocytes" . Molecular and Cellular Biochemistry, vol. 43, no. 3, 1982, pp. 161-166.http://dx.doi.org/10.1007/BF00223007
---------- VANCOUVER ----------
Téllez-Iñón, M.T., Torres, H.N. Cyclic nucleotide phosphodiesterase activities in rat erythrocytes. Mol Cell Biochem. 1982;43(3):161-166.http://dx.doi.org/10.1007/BF00223007
