Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Lead (Pb) is an environmental and industrial contaminant that still represents a public health problem. Elevated Pb exposure has been inversely correlated with femoral bone density and associated with osteoporosis. In the last years, it has been shown that inhibition of osteogenesis from mesenchymal stem cells activates adipogenesis and vice versa. In this paper, we investigated the effect of Pb on the differentiation of 3T3-L1 fibroblasts to adipocytes which is the cell model most used to study adipogenesis. After induction of differentiation, 2 days post-confluent cells re-enter the cell cycle and undergo mitotic clonal expansion (MCE) followed by expression of genes that produce the adipocyte phenotype. The presence of concentrations of Pb up to 10 μM during differentiation of 3T3-L1 fibroblasts did not interfere with MCE but enhanced the accumulation of cytosolic lipids that occur during adipogenesis, as well as, the induction of PPARγ, the master gene in adipogenesis. It is known that PPARγ upregulation is subsequent to induction of C/EBPβ and ERK activation, which are early events in adipogenesis. We found that both events were enhanced by Pb treatment. Our results support a stimulatory effect of Pb on adipogenesis which involves ERK activation and C/EBPβ upregulation prior to PPARγ and adipogenesis activation. © 2017, Springer Science+Business Media, LLC.

Registro:

Documento: Artículo
Título:Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation
Autor:Martini, C.N.; Gabrielli, M.; Bonifacino, G.; Codesido, M.M.; Vila, M.D.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Universidad de Buenos Aires, CONICET, Buenos Aires, Argentina
Palabras clave:3T3-L1 fibroblasts; Adipogenesis; ERK; Lead; PPARγ; CCAAT enhancer binding protein beta; lead; mitogen activated protein kinase; peroxisome proliferator activated receptor gamma; CCAAT enhancer binding protein beta; Cebpb protein, mouse; lead; peroxisome proliferator activated receptor gamma; 3T3-L1 cell line; adipocyte; adipogenesis; animal cell; Article; cell differentiation; enzyme activation; gene expression; mitosis; nonhuman; phenotype; adipocyte; animal; drug effect; fibroblast; MAPK signaling; metabolism; mouse; pathology; 3T3-L1 Cells; Adipocytes; Adipogenesis; Animals; CCAAT-Enhancer-Binding Protein-beta; Fibroblasts; Lead; MAP Kinase Signaling System; Mice; PPAR gamma
Año:2018
Volumen:437
Número:1-2
Página de inicio:37
Página de fin:44
DOI: http://dx.doi.org/10.1007/s11010-017-3093-y
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol. Cell. Biochem.
ISSN:03008177
CODEN:MCBIB
CAS:lead, 7439-92-1, 13966-28-4; mitogen activated protein kinase, 142243-02-5; CCAAT-Enhancer-Binding Protein-beta; Cebpb protein, mouse; Lead; PPAR gamma
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v437_n1-2_p37_Martini

Referencias:

  • Mahaffey, K.R., Annest, J.L., Roberts, J., Murphy, R.S., National estimates of blood lead levels: United States, 1976–1980: association with selected demographic and socioeconomic factors (1982) N Engl J Med, 307, pp. 573-579. , COI: 1:STN:280:DyaL383nt1Whtw%3D%3D, PID: 7110203
  • Needleman, H., Lead poisoning (2004) Annu Rev Med, 55, pp. 209-222. , COI: 1:CAS:528:DC%2BD2cXitVWrtbY%3D, PID: 14746518
  • Martínez, S.A., Simonella, L., Hansen, C., Rivolta, S., Cancela, L.M., Virgolini, M.B., Blood lead levels and enzymatic biomarkers of environmental lead exposure in children in Cordoba, Argentina, after the ban of leaded gasoline (2013) Hum Exp Toxicol, 32, pp. 449-463. , PID: 23079669
  • Liu, J., Ai, Y., McCauley, L., Pinto-Martin, J., Yan, C., Shen, X., Needleman, H., Blood lead levels and associated sociodemographic factors among preschool children in the South Eastern region of China (2012) Paediatr Perinat Epidemiol, 26, pp. 61-69. , PID: 22150709
  • Driscoll, T.R., Carey, R.N., Peters, S., Glass, D.C., Benke, G., Reid, A., Fritschi, L., The Australian work exposures study: occupational exposure to lead and lead compounds (2015) Ann Occup Hyg
  • Kašuba, V., Rozgaj, R., Milić, M., Zelježić, D., Kopjar, N., Pizent, A., Kljaković-Gašpić, Z., Jazbec, A., Evaluation of genotoxic effects of lead in pottery-glaze workers using micronucleus assay, alkaline comet assay and DNA diffusion assay (2012) Int Arch Occup Environ Health, 85, pp. 807-818. , PID: 22160131
  • Landrigan, P., Nordberg, M., Lucchini, R., Nordberg, G., Grandjean, P., Iregren, A., Alessio, L., The declaration of Brescia on prevention of the neurotoxicity of metals June 18, 2006 (2007) Am J Ind Med, 50, pp. 709-711. , PID: 17036364
  • Murata, K., Iwata, T., Dakeishi, M., Karita, K., Lead toxicity: does the critical level of lead resulting in adverse effects differ between adults and children? (2009) J Occup Health, 51, pp. 1-12. , COI: 1:CAS:528:DC%2BD1MXjtVylsb4%3D, PID: 18987427
  • Nash, D., Magder, L.S., Sherwin, R., Rubin, R.J., Silbergeld, E.K., Bone density-related predictors of blood lead level among peri- and postmenopausal women in the United States: the Third National Health and Nutrition Examination Survey, 1988–1994 (2004) Am J Epidemiol, 160, pp. 901-911. , PID: 15496543
  • Campbell, J.R., Auinger, P., The association between blood lead levels and osteoporosis among adults results from the third national health and nutrition examination survey (NHANES III) (2007) Environ Health Perspect, 115, pp. 1018-1022. , COI: 1:CAS:528:DC%2BD2sXosFSjsr8%3D, PID: 17637916
  • Abdullah, M., Rahman, F.A., Gnanasegaran, N., Govindasamy, V., Abu Kasim, N.H., Musa, S., Diverse effects of lead nitrate on the proliferation, differentiation, and gene expression of stem cells isolated from a dental origin (2014) Sci World J
  • Tang, Q.Q., Otto, T.C., Lane, M.D., Mitotic clonal expansion: a synchronous process required for adipogenesis (2003) Proc Natl Acad Sci USA, 100, pp. 44-49. , COI: 1:CAS:528:DC%2BD3sXktlOisw%3D%3D, PID: 12502791
  • Martini, C.N., Plaza, M.V., Vila, M.C., PKA-dependent and independent cAMP signalling in 3T3-L1 fibroblasts differentiation (2009) Mol Cell Endocrinol, 298, pp. 42-47. , COI: 1:CAS:528:DC%2BD1cXhsFCms7bM, PID: 19010385
  • Farmer, S.R., Transcriptional control of adipocyte formation (2006) Cell Metab, 4, pp. 263-273. , COI: 1:CAS:528:DC%2BD28XhtFCgtrfK, PID: 17011499
  • Gabrielli, M., Martini, C.N., Brandani, J.N., Iustman, L.J.R., Romero, D.G., Vila, M.C., Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation (2014) Dev Growth Differ, 56, pp. 143-151. , COI: 1:CAS:528:DC%2BC2cXisV2nsrw%3D, PID: 24444094
  • Wang, Z.X., Jiang, C.S., Liu, L., Wang, X.H., Jin, H.J., Wu, Q., Chen, Q., The role of AKT on arsenic trioxide suppression of 3T3-L1 preadipocyte differentiation (2005) Cell Res, 15, pp. 379-386. , COI: 1:CAS:528:DC%2BD2MXhtFWnsbrF, PID: 15916724
  • Martini, C.N., Gabrielli, M., Vila, M.C., A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts (2012) Toxicol In Vitro, 26, pp. 1007-1013. , COI: 1:CAS:528:DC%2BC38Xms12rt7g%3D, PID: 22546541
  • Martini, C.N., Brandani, J.N., Gabrielli, M., Vila, M.C., Effect of hexavalent chromium on proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts (2014) Toxicol In Vitro, 28, pp. 700-706. , COI: 1:CAS:528:DC%2BC2cXmtVaisbk%3D, PID: 24576443
  • Martini, C.N., Gabrielli, M., Brandani, J.N., Vila, M.C., Glyphosate inhibits PPARγ induction and differentiation of preadipocytes and is able to induce oxidative stress (2016) J Biochem Mol Toxicol, 30 (8), pp. 404-413. , COI: 1:CAS:528:DC%2BC28XltlamtLw%3D, PID: 27044015
  • Student, A.K., Hsu, R.Y., Lane, M.D., Induction of fatty acid synthetase synthesis in differentiating 3T3-L1 preadipocytes (1980) J Biol Chem, 255, pp. 4745-4750. , COI: 1:CAS:528:DyaL3cXksVamtr8%3D, PID: 7372608
  • Ramirez-Zacarías, J.L., Castro-Muñozledo, F., Kuri-Harcuch, W., Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O (1992) Histochemistry, 97, pp. 493-497. , PID: 1385366
  • Zhang, L., Paddon, C., Lewis, M.D., Grennan-Jones, F., Ludgate, M., Gsalpha signalling suppresses PPARgamma2 generation and inhibits 3T3L1 adipogenesis (2009) J Endocrinol, 202, pp. 207-215. , COI: 1:CAS:528:DC%2BD1MXpsFOgsbw%3D, PID: 19460852
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685. , COI: 1:CAS:528:DC%2BD3MXlsFags7s%3D, PID: 5432063
  • Takahashi, Y., Shinoda, A., Furuya, N., Harada, E., Arimura, N., Ichi, I., Fujiwara, Y., Sato, R., Perilipin-mediated lipid droplet formation in adipocytes promotes sterol regulatory element-binding protein-1 processing and triacylglyceride accumulation (2013) PLoS ONE, 8 (5). , COI: 1:CAS:528:DC%2BC3sXpsFemsro%3D, PID: 23734208
  • Arimura, N., Horiba, T., Imagawa, M., Shimizu, M., Sato, R., The peroxisome proliferator-activated receptor gamma regulates expression of the Perilipin gene in adipocytes (2004) J Biol Chem, 279, pp. 10070-10076. , COI: 1:CAS:528:DC%2BD2cXhvFCitLk%3D, PID: 14704148
  • Prusty, D., Park, B.H., Davis, K.E., Farmer, S.R., Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARγ) and C/EBPalpha gene expression during the differentiation of 3T3-L1 preadipocytes (2002) J Biol Chem, 277, pp. 46226-46232. , COI: 1:CAS:528:DC%2BD38XovFKhs74%3D, PID: 12270934
  • Park, B.H., Qiang, L., Farmer, S.R., Phosphorylation of C/EBPβ at a consensus extracellular signal-regulated kinase/glycogen synthase kinase 3 site is required for the induction of adiponectin gene expression during the differentiation of mouse fibroblasts into adipocytes (2004) Mol Cell Biol, 24, pp. 8671-8680. , COI: 1:CAS:528:DC%2BD2cXnvVaktb0%3D, PID: 15367685
  • Beier, E.E., Maher, J.R., Sheu, T.J., Cory-Slechta, D.A., Berger, A.J., Zuscik, M.J., Puzas, J.E., Heavy metal lead exposure, osteoporotic-like phenotype in an animal model, and depression of Wnt signaling (2013) Environ Health Perspect, 121, pp. 97-104. , PID: 23086611
  • Beier, E.E., Inzana, J.A., Sheu, T.J., Shu, L., Puzas, J.E., Mooney, R.A., Effects of combined exposure to lead and high-fat diet on bone quality in juvenile male mice (2015) Environ Health Perspect, 123, pp. 935-943. , PID: 25861094
  • Atashi, F., Modarressi, A., Pepper, M.S., The role of reactive oxygen species in mesenchymal stem cell adipogenic and osteogenic differentiation: a review (2015) Stem Cells Dev, 24, pp. 1150-1163. , COI: 1:CAS:528:DC%2BC2MXnsVanurc%3D, PID: 25603196
  • Chung, S.S., Lee, J.S., Kim, M., Ahn, B.Y., Jung, H.S., Lee, H.M., Kim, J.W., Park, K.S., Regulation of Wnt/β-catenin signaling by CCAAT/enhancer binding protein β during adipogenesis (2012) Obesity, 20, pp. 482-487. , COI: 1:CAS:528:DC%2BC38XivVenu7k%3D, PID: 21760632
  • Li, X., Kim, J.W., Grønborg, M., Urlaub, H., Lane, M.D., Tang, Q.Q., Role of cdk2 in the sequential phosphorylation/activation of C/EBPβ during adipocyte differentiation (2007) Proc Natl Acad Sci USA, 104, pp. 11597-11602. , COI: 1:CAS:528:DC%2BD2sXotVShsr0%3D, PID: 17601773
  • Cordova, F.M., Rodrigues, A.L., Giacomelli, M.B., Oliveira, C.S., Posser, T., Dunkley, P.R., Leal, R.B., Lead stimulates ERK1/2 and p38MAPK phosphorylation in the hippocampus of immature rats (2004) Brain Res, 998 (1), pp. 65-72. , COI: 1:CAS:528:DC%2BD2cXjsV2mug%3D%3D, PID: 14725969
  • Zhang, Q., Bratton, G.R., Agarwal, R.K., Calise, D., Kugel, G., Wan, Y., Kumar, M.A., Lead-induced cell signaling cascades in GT1-7 cells (2003) Brain Res Bull, 61, pp. 207-217. , COI: 1:CAS:528:DC%2BD3sXkvVKitbY%3D, PID: 12832008
  • Lin, Y.W., Chuang, S.M., Yang, L.Y., Persistent activation of ERK1/2 by lead acetate increases nucleotide excision repair synthesis and confers anti-cytotoxicity and anti-mutagenicity (2003) Carcinogenesis, 24 (1), pp. 53-61. , PID: 12538349

Citas:

---------- APA ----------
Martini, C.N., Gabrielli, M., Bonifacino, G., Codesido, M.M. & Vila, M.D.C. (2018) . Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation. Molecular and Cellular Biochemistry, 437(1-2), 37-44.
http://dx.doi.org/10.1007/s11010-017-3093-y
---------- CHICAGO ----------
Martini, C.N., Gabrielli, M., Bonifacino, G., Codesido, M.M., Vila, M.D.C. "Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation" . Molecular and Cellular Biochemistry 437, no. 1-2 (2018) : 37-44.
http://dx.doi.org/10.1007/s11010-017-3093-y
---------- MLA ----------
Martini, C.N., Gabrielli, M., Bonifacino, G., Codesido, M.M., Vila, M.D.C. "Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation" . Molecular and Cellular Biochemistry, vol. 437, no. 1-2, 2018, pp. 37-44.
http://dx.doi.org/10.1007/s11010-017-3093-y
---------- VANCOUVER ----------
Martini, C.N., Gabrielli, M., Bonifacino, G., Codesido, M.M., Vila, M.D.C. Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation. Mol. Cell. Biochem. 2018;437(1-2):37-44.
http://dx.doi.org/10.1007/s11010-017-3093-y