Artículo

Ceruti, J.M.; Ogara, M.F.; Menéndez, C.; Palmero, I.; Cánepa, E.T. "Inhibitor of growth 1 (ING1) acts at early steps of multiple DNA repair pathways" (2013) Molecular and Cellular Biochemistry. 378(1-2):117-126
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Abstract:

ING proteins are tumor suppressors involved in the regulation of gene transcription, cell cycle arrest, apoptosis, and senescence. Here, we show that ING1b expression is upregulated by several DNA-damaging agents, in a p53-independent manner. ING1b stimulates DNA repair of a variety of DNA lesions requiring activation of multiple DNA repair pathways. Moreover, Ing1 -/- cells showed impaired genomic DNA repair after H 2O2 and neocarzinostatin treatment and this defect was reverted by overexpression of ING1b. Two tumor-derived ING1 mutants failed to promote DNA repair highlighting the physiological importance of the integrity of the PHD domain for ING1b DNA repair activity and suggesting a role in the prevention of tumor progression. Ing-/- cells showed higher basal levels of γ-H2AX and, upon DNA damage, γ-H2AX increase was greater and with faster kinetics compared to wild-type cells. Chromatin relaxation by Trichostatin A led to an exacerbated damage signal in both types of cells, but this effect was dependent on Ing1 status, and more pronounced in wild-type cells. Our results suggest that ING1 acts at early stages of the DNA damage response activating a variety of repair mechanisms and that this function of ING1 is targeted in tumors.

Registro:

Documento: Artículo
Título:Inhibitor of growth 1 (ING1) acts at early steps of multiple DNA repair pathways
Autor:Ceruti, J.M.; Ogara, M.F.; Menéndez, C.; Palmero, I.; Cánepa, E.T.
Filiación:Laboratorio de Biología Molecular, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Arturo Duperier, 4, 28029 Madrid, Spain
Spanish National Cancer Research Centre (CNIO), Melchor Fernández Almagro, 3, 28029 Madrid, Spain
Palabras clave:Checkpoint kinase 1; Chromatin structure; DNA damage response; DNA repair; Genotoxic stress; ING1; P53; beta tubulin; gamma H2AX; histone H2AX; hydrogen peroxide; inhibitor of growth 1 protein; messenger RNA; mutant protein; protein ING1b; protein p16; protein p21; protein p53; trichostatin A; tumor suppressor protein; unclassified drug; zinostatin; checkpoint kinase 1; gamma H2AX protein, mouse; gamma-H2AX protein, mouse; histone; ING1 protein, human; isoprotein; nuclear protein; protein kinase; signal peptide; tumor suppressor protein; animal cell; apoptosis; article; cell cycle arrest; cell type; controlled study; DNA damage; DNA repair; gene expression regulation; human; human cell; Ing1b gene; nonhuman; protein domain; protein expression; senescence; transcription regulation; tumor growth; tumor suppressor gene; upregulation; wild type; animal; gene expression; genomic instability; human genome; metabolism; missense mutation; mouse; physiology; tumor cell line; Animals; Cell Line, Tumor; DNA Damage; DNA Repair; Gene Expression; Genome, Human; Genomic Instability; Histones; Humans; Intracellular Signaling Peptides and Proteins; Mice; Mutation, Missense; Nuclear Proteins; Protein Isoforms; Protein Kinases; Tumor Suppressor Proteins; Up-Regulation
Año:2013
Volumen:378
Número:1-2
Página de inicio:117
Página de fin:126
DOI: http://dx.doi.org/10.1007/s11010-013-1601-2
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol. Cell. Biochem.
ISSN:03008177
CODEN:MCBIB
CAS:beta tubulin, 87090-36-6; hydrogen peroxide, 7722-84-1; protein p21, 85306-28-1; trichostatin A, 58880-19-6; zinostatin, 9014-02-2; histone, 9062-68-4; protein kinase, 9026-43-1; Checkpoint kinase 1, 2.7.11.1; Histones; ING1 protein, human; Intracellular Signaling Peptides and Proteins; Nuclear Proteins; Protein Isoforms; Protein Kinases, 2.7.-; Tumor Suppressor Proteins; gamma-H2AX protein, mouse
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v378_n1-2_p117_Ceruti

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Citas:

---------- APA ----------
Ceruti, J.M., Ogara, M.F., Menéndez, C., Palmero, I. & Cánepa, E.T. (2013) . Inhibitor of growth 1 (ING1) acts at early steps of multiple DNA repair pathways. Molecular and Cellular Biochemistry, 378(1-2), 117-126.
http://dx.doi.org/10.1007/s11010-013-1601-2
---------- CHICAGO ----------
Ceruti, J.M., Ogara, M.F., Menéndez, C., Palmero, I., Cánepa, E.T. "Inhibitor of growth 1 (ING1) acts at early steps of multiple DNA repair pathways" . Molecular and Cellular Biochemistry 378, no. 1-2 (2013) : 117-126.
http://dx.doi.org/10.1007/s11010-013-1601-2
---------- MLA ----------
Ceruti, J.M., Ogara, M.F., Menéndez, C., Palmero, I., Cánepa, E.T. "Inhibitor of growth 1 (ING1) acts at early steps of multiple DNA repair pathways" . Molecular and Cellular Biochemistry, vol. 378, no. 1-2, 2013, pp. 117-126.
http://dx.doi.org/10.1007/s11010-013-1601-2
---------- VANCOUVER ----------
Ceruti, J.M., Ogara, M.F., Menéndez, C., Palmero, I., Cánepa, E.T. Inhibitor of growth 1 (ING1) acts at early steps of multiple DNA repair pathways. Mol. Cell. Biochem. 2013;378(1-2):117-126.
http://dx.doi.org/10.1007/s11010-013-1601-2