Abstract:
Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate cyclase activity also contained binding activity for L-T3. Binding studies indicate the existence of two L-T3 receptor components associated to adenylate cyclase activity. The component exhibiting the highest hormone affinity has the lowest binding capacity. © 1981 Martinus Nijhoff/Dr W. Junk Publishers.
Registro:
Documento: |
Artículo
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Título: | Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations |
Autor: | Kornblihtt, A.R.; Flawiá, M.M.; de Mendoza, D.; Glikin, G.; Farías, R.; Torres, H.N. |
Filiación: | Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Fac. de Ciencias Exactas y Naturales, Universidad Nacional de Tucumán, Obligado 2490, Buenos Aires, 1428, Argentina Instituto de Química Biológica, Universidad Nacional de Tucumán, Obligado 2490, Buenos Aires, 1428, Argentina
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Palabras clave: | adenylate cyclase; liothyronine; animal experiment; male genital system; rat; testis; Adenylate Cyclase; Animals; Cytosol; Kinetics; Male; Protein Binding; Rats; Seminiferous Tubules; Testis; Triiodothyronine; Animalia |
Año: | 1981
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Volumen: | 36
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Número: | 1
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Página de inicio: | 23
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Página de fin: | 27
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DOI: |
http://dx.doi.org/10.1007/BF02354828 |
Título revista: | Molecular and Cellular Biochemistry
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Título revista abreviado: | Mol Cell Biochem
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ISSN: | 03008177
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CODEN: | MCBIB
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CAS: | adenylate cyclase, 9012-42-4; liothyronine, 6138-47-2, 6893-02-3; Adenylate Cyclase, 4.6.1.1; Triiodothyronine, 6893-02-3
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v36_n1_p23_Kornblihtt |
Referencias:
- Limbrid, L.E., Lefkowitz, R.J., (1977) J. Biol. Chem., 252, pp. 799-802
- Haga, T., Haga, K., Gilman, A.G., (1977) J. Biol. Chem., 252, pp. 5776-5782
- Welton, A.F., Lad, P.M., Newby, A.C., Yamamura, N., Nicosia, S., Rodbell, M., (1977) J. Biol. Chem., 252, pp. 5947-5950
- Braun, T., Dods, R.F., (1975) Proc. Natl. Acad. Sci. U.S.A., 72, pp. 1097-1101
- Neer, E.J., (1978) J. Biol. Chem., 253, pp. 5808-5812
- Flawia, M. M., Kornblihtt, A., de Mendoza, D., & Torres, H. N., 1980. Mol. and Cell. Biochem; Lantham, K.R., Ring, J.C., Baxter, J.D., (1976) J. Biol. Chem., 251, pp. 7388-7397
- Worthington Enzyme Manual 1972. Worthington Biochemical Corporation: pp. 7–12, Freehold; Terenzi, H.F., Roselino, E., Passeron, S., (1971) Eur. J. Biochem., 18, pp. 342-350
Citas:
---------- APA ----------
Kornblihtt, A.R., Flawiá, M.M., de Mendoza, D., Glikin, G., Farías, R. & Torres, H.N.
(1981)
. Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations. Molecular and Cellular Biochemistry, 36(1), 23-27.
http://dx.doi.org/10.1007/BF02354828---------- CHICAGO ----------
Kornblihtt, A.R., Flawiá, M.M., de Mendoza, D., Glikin, G., Farías, R., Torres, H.N.
"Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations"
. Molecular and Cellular Biochemistry 36, no. 1
(1981) : 23-27.
http://dx.doi.org/10.1007/BF02354828---------- MLA ----------
Kornblihtt, A.R., Flawiá, M.M., de Mendoza, D., Glikin, G., Farías, R., Torres, H.N.
"Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations"
. Molecular and Cellular Biochemistry, vol. 36, no. 1, 1981, pp. 23-27.
http://dx.doi.org/10.1007/BF02354828---------- VANCOUVER ----------
Kornblihtt, A.R., Flawiá, M.M., de Mendoza, D., Glikin, G., Farías, R., Torres, H.N. Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations. Mol Cell Biochem. 1981;36(1):23-27.
http://dx.doi.org/10.1007/BF02354828