Artículo

Carcagno, A.L.; Giono, L.E.; Marazita, M.C.; Castillo, D.S.; Pregi, N.; Cánepa, E.T. "E2F1 induces p19INK4d, a protein involved in the DNA damage response, following UV irradiation" (2012) Molecular and Cellular Biochemistry. 366(1-2):123-129
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Abstract:

Central to the maintenance of genomic integrity is the cellular DNA damage response. Depending on the type of genotoxic stress and through the activation of multiple signaling cascades, it can lead to cell cycle arrest, DNA repair, senescence, and apoptosis. p19INK4d, a member of the INK4 family of CDK inhibitors, plays a dual role in the DNA damage response, inhibiting cell proliferation and promoting DNA repair. Consistently, p19INK4d has been reported to become upregulated in response to UV irradiation and a great variety of genotoxic agents. Here, this induction is shown to result from a transcriptional stimulatory mechanism that can occur at every phase of the cell cycle except during mitosis. Moreover, evidence is presented that demonstrates that E2F1 is involved in the induction of p19INK4d following UV treatment, as it is prevented by E2F1 protein ablation and DNA-binding inhibition. Specific inhibition of this regulation using triplex-forming oligonucleotides that target the E2F response elements present in the p19INK4d promoter also block p19INK4d upregulation and sensitize cells to DNA damage. These results constitute the first description of a mechanism for the induction of p19INK4d in response to UV irradiation and demonstrate the physiological relevance of this regulation following DNA damage. © 2012 Springer Science+Business Media, LLC.

Registro:

Documento: Artículo
Título:E2F1 induces p19INK4d, a protein involved in the DNA damage response, following UV irradiation
Autor:Carcagno, A.L.; Giono, L.E.; Marazita, M.C.; Castillo, D.S.; Pregi, N.; Cánepa, E.T.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, 1428 Ciudad de Buenos Aires, Argentina
Palabras clave:DNA damage response; E2F1; p19INK4d; Transcriptional regulation; UV irradiation; beta tubulin; caspase 3; cyclin dependent kinase inhibitor; cyclin E; protein p191nk4d; tamoxifen; transcription factor E2F2; unclassified drug; animal cell; apoptosis; article; cell cycle arrest; cell cycle phase; cell proliferation; clonogenic assay; controlled study; DNA binding; DNA damage; DNA repair; DNA responsive element; enzyme activity; enzyme induction; enzyme inhibition; gene sequence; genetic transfection; genomic instability; genotoxicity; human; human cell; mitosis; nonhuman; regulatory mechanism; RNA extraction; transcription regulation; ultraviolet irradiation; upregulation; Animals; Cell Cycle; Cricetinae; Cyclin-Dependent Kinase Inhibitor p19; DNA; DNA Damage; DNA Repair; E2F1 Transcription Factor; HEK293 Cells; Humans; Response Elements; Transcription, Genetic; Transcriptional Activation; Ultraviolet Rays
Año:2012
Volumen:366
Número:1-2
Página de inicio:123
Página de fin:129
DOI: http://dx.doi.org/10.1007/s11010-012-1289-8
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol. Cell. Biochem.
ISSN:03008177
CODEN:MCBIB
CAS:beta tubulin, 87090-36-6; caspase 3, 169592-56-7; tamoxifen, 10540-29-1; Cyclin-Dependent Kinase Inhibitor p19; DNA, 9007-49-2; E2F1 Transcription Factor; E2F1 protein, human; triplex DNA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v366_n1-2_p123_Carcagno

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Citas:

---------- APA ----------
Carcagno, A.L., Giono, L.E., Marazita, M.C., Castillo, D.S., Pregi, N. & Cánepa, E.T. (2012) . E2F1 induces p19INK4d, a protein involved in the DNA damage response, following UV irradiation. Molecular and Cellular Biochemistry, 366(1-2), 123-129.
http://dx.doi.org/10.1007/s11010-012-1289-8
---------- CHICAGO ----------
Carcagno, A.L., Giono, L.E., Marazita, M.C., Castillo, D.S., Pregi, N., Cánepa, E.T. "E2F1 induces p19INK4d, a protein involved in the DNA damage response, following UV irradiation" . Molecular and Cellular Biochemistry 366, no. 1-2 (2012) : 123-129.
http://dx.doi.org/10.1007/s11010-012-1289-8
---------- MLA ----------
Carcagno, A.L., Giono, L.E., Marazita, M.C., Castillo, D.S., Pregi, N., Cánepa, E.T. "E2F1 induces p19INK4d, a protein involved in the DNA damage response, following UV irradiation" . Molecular and Cellular Biochemistry, vol. 366, no. 1-2, 2012, pp. 123-129.
http://dx.doi.org/10.1007/s11010-012-1289-8
---------- VANCOUVER ----------
Carcagno, A.L., Giono, L.E., Marazita, M.C., Castillo, D.S., Pregi, N., Cánepa, E.T. E2F1 induces p19INK4d, a protein involved in the DNA damage response, following UV irradiation. Mol. Cell. Biochem. 2012;366(1-2):123-129.
http://dx.doi.org/10.1007/s11010-012-1289-8