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Abstract:

Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally a sharp enzyme inhibition is evident at increasing hormone concentrations from 10-6 to 10-4 M. Incubation of decapsulated testes with L-T3 leads to a decrease of intracellular cyclic AMP levels. Dose-response relationships for such effect are similar to those found for adenylate cyclase activity. In this case a clear response is observed at 10-8 M L-T3. © 1981 Martinus Nijhoff Dr. W. Juk Publishers B.V.

Registro:

Documento: Artículo
Título:Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine
Autor:Flawia, M.M.; Kornblihtt, A.R.; de Mendoza, D.; Torres, H.N.
Filiación:Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Obligado 2490, Buenos Aires, 1428, Argentina
Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:adenylate cyclase; cyclic amp; liothyronine; animal experiment; drug response; endocrine system; enzyme inhibition; hormone sensitivity; in vitro study; male genital system; preliminary communication; rat; seminiferous tubule; testis; Adenylate Cyclase; Animal; Cyclic AMP; Cytosol; Dose-Response Relationship, Drug; Hydrogen-Ion Concentration; Kinetics; Magnesium; Male; Manganese; Rats; Subcellular Fractions; Support, Non-U.S. Gov't; Testis; Triiodothyronine
Año:1981
Volumen:34
Número:3
Página de inicio:185
Página de fin:190
DOI: http://dx.doi.org/10.1007/BF02359623
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:adenylate cyclase, 9012-42-4; cyclic AMP, 60-92-4; liothyronine, 6138-47-2, 6893-02-3; Adenylate Cyclase, EC 4.6.1.1; Cyclic AMP, 60-92-4; Magnesium, 7439-95-4; Manganese, 7439-96-5; Triiodothyronine, 6893-02-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v34_n3_p185_Flawia

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Citas:

---------- APA ----------
Flawia, M.M., Kornblihtt, A.R., de Mendoza, D. & Torres, H.N. (1981) . Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine. Molecular and Cellular Biochemistry, 34(3), 185-190.
http://dx.doi.org/10.1007/BF02359623
---------- CHICAGO ----------
Flawia, M.M., Kornblihtt, A.R., de Mendoza, D., Torres, H.N. "Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine" . Molecular and Cellular Biochemistry 34, no. 3 (1981) : 185-190.
http://dx.doi.org/10.1007/BF02359623
---------- MLA ----------
Flawia, M.M., Kornblihtt, A.R., de Mendoza, D., Torres, H.N. "Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine" . Molecular and Cellular Biochemistry, vol. 34, no. 3, 1981, pp. 185-190.
http://dx.doi.org/10.1007/BF02359623
---------- VANCOUVER ----------
Flawia, M.M., Kornblihtt, A.R., de Mendoza, D., Torres, H.N. Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine. Mol Cell Biochem. 1981;34(3):185-190.
http://dx.doi.org/10.1007/BF02359623