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Abstract:

The subcellular localization and characterization of some of the components involved in the glycosylation of asparagine type glycoproteins was attempted using dolichyl diphosphate [14c]mannose oligosaccharide as precursor of the glycosylation reaction in vitro. Isolated rough and smooth microsomel fractions were able to carry out the transfer of the carbohydrate moiety from lipid oligosaccharide to endogenous protein acceptors. The protein glycosylating activity remained practically the same after stripping the vesicles from their ribosomes or partially releasing their vesicular content. Isolation of polysomes from rough microsomes after glycosylation has taken place, reveals that a large proportion of mannose labeled glycoproteins is in the membranous fraction. The remaining labeled glycoproteins co-sediment with the polysomal fraction. If the isolation is carried out before glycosylation only the membranous fraction shows enzyme activity, whereas the polysomes alone are not able to carry out glycosylation. All these results taken together indicate that the protein glycosylating enzyme is a structural component of the rough and smooth microsomes of rat liver. © 1979 Dr. W. Junk b.v. Publishers.

Registro:

Documento: Artículo
Título:Oligosaccharide transfer from lipid sugar intermediates to endogenous protein(s) of rat liver microsomal subfractions
Autor:Idoyaga-Vargas, V.; Perelmuter, M.; Burrone, O.; Carminatti, H.
Filiación:Instituto de Investigaciones Bioquímicas 'Fundación Campomar', and Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:drug derivative; glycoprotein; isoprenoid phosphate sugar; mannose; membrane protein; animal; article; biosynthesis; enzymology; intracellular membrane; liver microsome; metabolism; polysome; rat; Animal; Glycoproteins; Intracellular Membranes; Mannose; Membrane Proteins; Microsomes, Liver; Polyisoprenyl Phosphate Oligosaccharides; Polyisoprenyl Phosphate Sugars; Polyribosomes; Rats; Support, U.S. Gov't, P.H.S.
Año:1979
Volumen:26
Número:2
Página de inicio:123
Página de fin:130
DOI: http://dx.doi.org/10.1007/BF00232890
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:mannose, 31103-86-3, 3458-28-4; Glycoproteins; Mannose, 31103-86-3; Membrane Proteins; Polyisoprenyl Phosphate Oligosaccharides; Polyisoprenyl Phosphate Sugars
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v26_n2_p123_IdoyagaVargas

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Citas:

---------- APA ----------
Idoyaga-Vargas, V., Perelmuter, M., Burrone, O. & Carminatti, H. (1979) . Oligosaccharide transfer from lipid sugar intermediates to endogenous protein(s) of rat liver microsomal subfractions. Molecular and Cellular Biochemistry, 26(2), 123-130.
http://dx.doi.org/10.1007/BF00232890
---------- CHICAGO ----------
Idoyaga-Vargas, V., Perelmuter, M., Burrone, O., Carminatti, H. "Oligosaccharide transfer from lipid sugar intermediates to endogenous protein(s) of rat liver microsomal subfractions" . Molecular and Cellular Biochemistry 26, no. 2 (1979) : 123-130.
http://dx.doi.org/10.1007/BF00232890
---------- MLA ----------
Idoyaga-Vargas, V., Perelmuter, M., Burrone, O., Carminatti, H. "Oligosaccharide transfer from lipid sugar intermediates to endogenous protein(s) of rat liver microsomal subfractions" . Molecular and Cellular Biochemistry, vol. 26, no. 2, 1979, pp. 123-130.
http://dx.doi.org/10.1007/BF00232890
---------- VANCOUVER ----------
Idoyaga-Vargas, V., Perelmuter, M., Burrone, O., Carminatti, H. Oligosaccharide transfer from lipid sugar intermediates to endogenous protein(s) of rat liver microsomal subfractions. Mol Cell Biochem. 1979;26(2):123-130.
http://dx.doi.org/10.1007/BF00232890