Navegar

Colección

Datos Estadísticas

Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The interaction of estradiol-receptor complexes and isolated human breast tumor chromatin was studied under equilibrium conditions. The estradiol-receptor complexes bound specifically to the chromatin of hormone dependent tumors and showed a single class of binding sites with a Ka of 0.96 × 1010m-1 and a binding capacity of 1.5 pmoles/mg DNA. The binding was a temperature-dependent process and involved a "transformation" of the receptor protein. The heat-activated hormone-receptor com-plex was more active than the 8S form in the binding phenomenon. The specific interaction of estradiol-receptor complex with isolated chromatin was saturable and sensitive to conditions of temperature and ionic strength. Furthermore under optimal conditions no acceptor sites were detected in chromatin of hormone independent tumors. © 1977 Dr. W. Junk b.v. Publishers.

Registro:

Documento: Artículo
Título:Binding of estradiol receptor complexes to isolated human breast chromatin
Autor:Charreau, E.H.; Baldi, A.
Filiación:Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:albumin i 125; dna; drug receptor; estradiol receptor; radioisotope; breast; breast carcinoma; chromatin; estradiol h 3; human; normal human; rna c 14; theoretical study; Breast Neoplasms; Chromatin; DNA, Neoplasm; Estradiol; Female; Human; Kinetics; Osmolar Concentration; Receptors, Estrogen; Sodium Chloride; Temperature
Año:1977
Volumen:16
Número:2-3
Página de inicio:79
Página de fin:86
DOI: http://dx.doi.org/10.1007/BF01732047
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:DNA, 9007-49-2; Chromatin; DNA, Neoplasm; Estradiol, 50-28-2; Receptors, Estrogen; Sodium Chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p79_Charreau

Referencias:

  • Jensen, E.V., De Sombre, E.R., (1972) A. Rev. Biochem., 41, pp. 203-230
  • Jensen, E.V., Suzuki, T., Kawashima, T., Stumpf, W.E., Jungblut, P.W., De Sombre, E.R., (1968) Proc. Nat. Acad. Sci. USA, 59, pp. 632-638
  • Giannopoulos, G., Gorski, J., (1971) J. Biol. Chem., 246, pp. 2530-2536
  • Jensen, E.V., De Sombre, E.R., (1973) Science, 182, pp. 126-134
  • Harris, G.S., (1971) Nature New Biol., 231, pp. 246-248
  • King, R.J.B., Gordon, J., (1972) Nature New Biol., 240, pp. 185-186
  • Toft, D.O., (1972) J. Steroid Biochem., 3, pp. 515-522
  • Puca, G.A., Sica, V., Nola, E., (1974) Proc. Nat. Acad. Sci., USA, 71, pp. 979-983
  • Spelsberg, T.C., Steggles, A.W., O'Malley, B.W., (1971) J. Biol. Chem., 246, pp. 4188-4197
  • Liang, T., Liao, S., (1974) J. Biol. Chem., 249, pp. 4671-4678
  • Liao, S., Liang, T., Tymoczko, J.L., (1973) Nature New Biol., 24, pp. 211-213
  • Jackson, V., Chalkley, G.R., (1974) J. Biol. Chem., 249, pp. 1615-1627
  • “Estrogen Receptors in Human Breast Cancer”, McGuire W. L., Carbone P. P. and Vollmer E. P. editors. Raven Press New York 1975; Toft, D., Gorski, J., (1966) Proc. Nat. Acad. Sci. USA, 55, pp. 1574-1581
  • Marmur, J., (1961) J. Mol. Biol., 3, pp. 208-218
  • Britten, R., Graham, D.E., Neufed, B.R., (1974) Methods in Enzymology, pp. 363-418. , L., Grossman, K., Moldave, Acad. Press, N.Y
  • Simpson, R.T., Sober, H., (1970) Biochemistry, 9, pp. 3103-3109
  • Bartley, J., Chalkley, R., (1970) J. Biol. Chem., 245, pp. 4286-4292
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275
  • Ceriotti, G., (1955) J. Biol. Chem., 214, pp. 59-70
  • Burton, K., (1956) Biochem. J., 62, pp. 315-323
  • Spelsberg, T.C., Hnilica, L.S., Ansevin, A.T., (1971) Biochim. Biophys. Acta, 228, pp. 550-562
  • Clark, R.J., Fensenfeld, G., (1971) Nature New Biology, 229, pp. 101-106
  • Puca, G.A., Nola, E., Hibner, U., Cicala, G., Sica, V., (1975) J. Biol. Chem., 250, pp. 6452-6458
  • Mainwaring, W.I.P., Synes, E.K., Higgins, S., (1976) Biochem. J., 156, pp. 129-141
  • Clark, J.H., Gorski, J., (1969) Biochim. Biophys Acta, 192, pp. 508-515
  • Erdos, T., Fires, J., (1974) Biochem. Biophys. Res. Commun., 58, pp. 932-939
  • Puca, G.A., Nola, E., Sica, V., Bresciani, F., (1972) Biochemistry, 11, pp. 4157-4165
  • Sala-Trepat, J.M., Vallet-Strove, C., (1974) Biochim Biophys Acta, 371, pp. 186-202
  • McGuire, W.L., Huff, K., Chamness, G.C., (1972) Biochemistry, 11, pp. 4562-4565
  • Jensen, E.W., Hurst, D.J., De Sombre, E.R., Jungblut, P.W., (1967) Science, 158, pp. 385-387
  • Puca, G.A., Bresciani, F., (1968) Nature, 218, pp. 967-969
  • Milgrom, E., Atger, A., Baulieu, E.E., (1973) Biochemistry, 12, pp. 5198-5205
  • Chamness, G.C., Jennings, A.W., McGuire, W.L., (1974) Biochemistry, 13, pp. 327-331
  • Chamness, G.C., Jennings, A.W., McGuire, W.L., (1973) Nature, 241, pp. 458-460
  • Spelsberg, T.C., Webster, R.A., Pikler, G.M., (1976) Nature, 262, pp. 65-67
  • Baldi, A., Charreau, E.H., (1975) Medicina, 35, p. 594

Citas:

---------- APA ----------
Charreau, E.H. & Baldi, A. (1977) . Binding of estradiol receptor complexes to isolated human breast chromatin. Molecular and Cellular Biochemistry, 16(2-3), 79-86.
http://dx.doi.org/10.1007/BF01732047
---------- CHICAGO ----------
Charreau, E.H., Baldi, A. "Binding of estradiol receptor complexes to isolated human breast chromatin" . Molecular and Cellular Biochemistry 16, no. 2-3 (1977) : 79-86.
http://dx.doi.org/10.1007/BF01732047
---------- MLA ----------
Charreau, E.H., Baldi, A. "Binding of estradiol receptor complexes to isolated human breast chromatin" . Molecular and Cellular Biochemistry, vol. 16, no. 2-3, 1977, pp. 79-86.
http://dx.doi.org/10.1007/BF01732047
---------- VANCOUVER ----------
Charreau, E.H., Baldi, A. Binding of estradiol receptor complexes to isolated human breast chromatin. Mol Cell Biochem. 1977;16(2-3):79-86.
http://dx.doi.org/10.1007/BF01732047