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Abstract:

Different Escherichia coli mutants auxotrophic for polyamines were studied in order to investigate the relationships among polypeptide synthesis in cell-free systems, ribosomal distribution profiles and endogenous polyamine pools. The in vitro protein synthetic activity and the polyribosomal content were reduced in extracts from putrescine-starved cells of the double mutants MA 255 and MA 261, but not in the arginine-conditional auxotroph DK 6. Putrescine addition to the cultures of all these strains previously starved for polyamines, provoked a shift towards monomers in the equilibrium involving ribosomal particles. Concomitant changes in the intracellular levels of polyamines were observed: putrescine and spermidine increased markedly, and cadaverine disappeared. © 1977 Dr. W. Junk b.v. Publishers.

Registro:

Documento: Artículo
Título:Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli
Autor:Goldemberg, S.H.; Algranati, I.D.
Filiación:Instituto de Investigaciones Bioquímicas 'Fundación Campomar', and Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:cadaverine; putrescine; spermidine; auxotrophic mutant; escherichia coli; in vitro study; microorganism; protein synthesis; theoretical study; Bacterial Proteins; Cell Division; Escherichia coli; Mutation; Putrescine; Ribosomes; Species Specificity; Time Factors; Translation, Genetic
Año:1977
Volumen:16
Número:2-3
Página de inicio:71
Página de fin:77
DOI: http://dx.doi.org/10.1007/BF01732046
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:cadaverine, 1476-39-7, 462-94-2; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; Bacterial Proteins; Putrescine, 110-60-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p71_Goldemberg

Referencias:

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Citas:

---------- APA ----------
Goldemberg, S.H. & Algranati, I.D. (1977) . Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli. Molecular and Cellular Biochemistry, 16(2-3), 71-77.
http://dx.doi.org/10.1007/BF01732046
---------- CHICAGO ----------
Goldemberg, S.H., Algranati, I.D. "Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli" . Molecular and Cellular Biochemistry 16, no. 2-3 (1977) : 71-77.
http://dx.doi.org/10.1007/BF01732046
---------- MLA ----------
Goldemberg, S.H., Algranati, I.D. "Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli" . Molecular and Cellular Biochemistry, vol. 16, no. 2-3, 1977, pp. 71-77.
http://dx.doi.org/10.1007/BF01732046
---------- VANCOUVER ----------
Goldemberg, S.H., Algranati, I.D. Polyamines and protein synthesis: Studies in various polyamine-requiring mutants of Escherichia coli. Mol Cell Biochem. 1977;16(2-3):71-77.
http://dx.doi.org/10.1007/BF01732046