Abstract:
Previous work showed that liver microsomes catalyze the transfer of glucose from dolichol monophosphate glucose to an endogenous acceptor believed to be a dolichol pyrophosphate derivative of an oligosaccharide. This oligosaccharide has now been prepared on a larger scale so as to permit the determination of its sugars. The purification procedure includes, as a last step, a thin layer chromatography on kieselguhr-silica gel to obviate glucose-containing contaminants. After complete hydrolysis mannose, glucose and a small amount of hexosamine were detected. © 1977 Dr. W. Junk b.v. Publishers.
Registro:
Documento: |
Artículo
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Título: | A lipid linked oligosaccharide which contains hexosamine, mannose and glucose |
Autor: | Tábora, E.; Behrens, N.H. |
Filiación: | Instituto de Investigaciones Bioquímicas, 'Fundación Campomar', and Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires (28), Argentina
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Palabras clave: | dolichol phosphate; glucose; hexosamine; lipid; mannose; dolichol diphosphate; in vitro study; theoretical study; Animal; Glucose; Hexosamines; Lipopolysaccharides; Liver; Mannose; Oligosaccharides; Phospholipids; Support, U.S. Gov't, P.H.S.; Swine |
Año: | 1977
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Volumen: | 16
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Número: | 2-3
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Página de inicio: | 193
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Página de fin: | 196
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DOI: |
http://dx.doi.org/10.1007/BF01732061 |
Título revista: | Molecular and Cellular Biochemistry
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Título revista abreviado: | Mol Cell Biochem
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ISSN: | 03008177
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CODEN: | MCBIB
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CAS: | dolichol phosphate, 12698-55-4, 34457-14-2; glucose, 50-99-7, 84778-64-3; lipid, 66455-18-3; mannose, 31103-86-3, 3458-28-4; Glucose, 50-99-7; Hexosamines; Lipopolysaccharides; Mannose, 31103-86-3; Oligosaccharides; Phospholipids
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p193_Tabora |
Referencias:
- Behrens, N.H., Parodi, A.J., Leloir, L.F., (1971) Proc. Nat. Acad. Sci. U.S.A., 68, pp. 2857-2860
- Parodi, A.J., Behrens, N.H., Leloir, L.F., Dankert, M., (1972) Biochim. Biophys. Acta, 270, pp. 529-536
- Parodi, A.J., Staneloni, R.J., Cantarella, A.I., Leloir, L.F., Carminatti, H., Levy, J.A., (1973) Carbohyd. Res., 26, pp. 393-400
- Behrens, N. H., 1974. in Biology and Chemistry of the Eucaryotic Cell Surface (E. Y. C. Lee and E. E. Smith, Eds.), Academic Press, pp. 159–180; Chen, P.S., Jr., Torbara, T.Y., Warner, H., (1956) Anal. Chem., 28, pp. 1756-1758
- Huber, C.N., Scobell, H.D., Tai, H., Fisher, E.E., Thin-layer chromatography of the malto-oligo and megalosaccharides with mixed support and multiple irrigations (1968) Analytical Chemistry, 40, pp. 207-209
- Shannon, J.C., Greech, R.G., (1969) J. Chromatog., 44, pp. 307-314
- Leloir, L.F., Cardini, C.E., (1953) Biochim. Biophys. Acta, 12, pp. 15-22
- Kates, M., 1972. in Laboratory Techniques in Biochemistry and Molecular (T. S. Work and E. Work, Eds.) North-Holland Publishing Co., 3, p. 438; Dumphy, P.J., Kerr, J.D., Pennock, J.F., Whittle, K.J., Feeney, J., The plurality of long chain isoprenoid alcohols (polyprenols) from natural sources (1967) Biochimica et Biophysica Acta (BBA) - General Subjects, 136, pp. 136-147
- Trevelyan, W.E., Procter, D.P., Harrison, J.S., (1950) Nature, 166, p. 444
- Isemura, Y., Schmid, K., (1971) Biochem. J., 124, pp. 591-602
- Parodi, A.J., Behrens, N.H., Leloir, L.F., Carminatti, H., (1972) Proc. Nat. Acad. Sci. U.S.A., 69, pp. 3268-3272
- Wagh, P.W., Roberts, B.I., (1972) Biochemistry, 11, pp. 4222-4227
- Adamany, A. M., 1976. Fed. Proceed. Abs. No 941
Citas:
---------- APA ----------
Tábora, E. & Behrens, N.H.
(1977)
. A lipid linked oligosaccharide which contains hexosamine, mannose and glucose. Molecular and Cellular Biochemistry, 16(2-3), 193-196.
http://dx.doi.org/10.1007/BF01732061---------- CHICAGO ----------
Tábora, E., Behrens, N.H.
"A lipid linked oligosaccharide which contains hexosamine, mannose and glucose"
. Molecular and Cellular Biochemistry 16, no. 2-3
(1977) : 193-196.
http://dx.doi.org/10.1007/BF01732061---------- MLA ----------
Tábora, E., Behrens, N.H.
"A lipid linked oligosaccharide which contains hexosamine, mannose and glucose"
. Molecular and Cellular Biochemistry, vol. 16, no. 2-3, 1977, pp. 193-196.
http://dx.doi.org/10.1007/BF01732061---------- VANCOUVER ----------
Tábora, E., Behrens, N.H. A lipid linked oligosaccharide which contains hexosamine, mannose and glucose. Mol Cell Biochem. 1977;16(2-3):193-196.
http://dx.doi.org/10.1007/BF01732061