Navegar

Colección

Datos Estadísticas

Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Several problems regarding the protein acceptor of the oligosaccharide from GEA (glucosylated endogenous acceptor) were investigated in the present work using rat liver microsomal subfractions. It was found that the acceptor molecule is present in rough and smooth liver microsomes. Furthermore both fractions have closely similar specific activities. The problem of whether nascent peptides must be ribosome bound for glycosylation to occur was studied. The results suggests that binding of peptides to ribosomes is not a necessary condition for the transfer of GEA oligosaccharide to protein. The increase in specific activity found after partial release of the microsomal vesicular content suggests that the acceptor protein for GEA is membrane bound. Evidence obtained in attempting to elucidate whether nascent or completed chains are glycosylated favours the later possibility. © 1977 Dr. W. Junk b.v. Publishers.

Registro:

Documento: Artículo
Título:Glycosylation of endogenous protein(s) of the rough and smooth microsomes by a lipid sugar intermediate
Autor:Idoyaga Vargas, V.; Carminatti, H.
Filiación:Instituto de Investigaciones Bioquimicas 'Fundación Campomar', and Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:glucose; lipid; protein; glycosylation; in vitro study; liver; microsome; rat; theoretical study; Animal; Deoxycholic Acid; Glycolipids; Glycoproteins; Kinetics; Male; Microsomes, Liver; Oligosaccharides; Puromycin; Rats; Ribosomes; Support, U.S. Gov't, P.H.S.
Año:1977
Volumen:16
Número:2-3
Página de inicio:171
Página de fin:176
DOI: http://dx.doi.org/10.1007/BF01732058
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:glucose, 50-99-7, 84778-64-3; lipid, 66455-18-3; protein, 67254-75-5; Deoxycholic Acid, 83-44-3; Glycolipids; Glycoproteins; Oligosaccharides; Puromycin, 53-79-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p171_IdoyagaVargas

Referencias:

  • Behrens, N.H., Leloir, L.F., (1970) Proc. Nat. Acad. Sci. U.S., 66, pp. 153-159
  • Behrens, N.H., Carminatti, H., Staneloni, R.J., Leloir, L.F., Cantarella, A.I., Formation of Lipid-Bound Oligosaccharides Containing Mannose. Their Role in Glycoprotein Synthesis (1973) Proceedings of the National Academy of Sciences, 70, pp. 3390-3394
  • Parodi, A.J., Behrens, N.H., Leloir, L.F., Carminatti, H., (1972) Proc. Nat. Acad. Sci. U.S., 69, pp. 3268-3272
  • Pless, D.D., Lennarz, W.J., (1975) J. Biol. Chem., 250, pp. 7014-7019
  • Hsu, A., Baynes, J.W., Heath, E.C., (1974) Proc. Nat. Acad. Sci. U.S., 71, pp. 2391-2395
  • Nikaido, H., Hassid, W.Z., (1971) Adv. Carbohyd. Chem. and Biochem., 26, pp. 351-483
  • Melchers, F., Biosynthesis, intracellular transport, and secretion of immunoglobulins. Effect of 2-deoxy-D-glucose in tumor plasma cells producing and secreting immunoglobulin G1 (1973) Biochemistry, 12, pp. 1471-1475
  • Molnar, J., Sy, D., (1967) Biochem., 6, pp. 1941-1947
  • Schachter, H., Jabbal, I., Hudgin, R.L., Pinteric, L., McGuire, E.J., Roseman, S., (1970) J. Biol. Chem., 245, pp. 1090-1100
  • Redman, C.M., Cherian, G., (1972) J. Cell Biol., 52, pp. 231-245
  • Tabora, E., 1975. Ph.D. Thesis. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires; Sunshine, G.H., Willia, D.J., Rabin, B.R., (1971) Nature New Biol., 230, pp. 133-136
  • Adelman, M.R., Blobel, G., Sabatini, D.D., (1974) Methods in Enzymol., 31, pp. 201-215
  • Kreibich, G., Sabatini, D.D., (1974) Methods in Enzymol., 31, pp. 215-225
  • Vassalli, P., Lisowka, K., Bernstein, B., Lamm, M.E., (1971) J. Mol. Biol., 56, pp. 1-19
  • Parodi, A.J., Staneloni, R., Cantarella, A.I., Leloir, L.F., Behrens, N.H., Carminatti, H., Levy, J.A., (1973) Carbohydr. Res., 26, pp. 393-400
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275
  • Campbell, P.N., Sargent, J.R., (1967) Techniques in Protein Biosynthesis, vol. 1, pp. 301-303. , Academic Press, New York
  • Behrens, N.H., Parodi, A.J., Leloir, L.F., Krisman, C.R., (1971) Arch. Biochem. Biophys., 143, pp. 375-383
  • Redman, C.M., Sabatini, D.D., (1966) Proc. Nat. Acad. Sci. U.S., 56, pp. 608-615
  • Adelman, M.R., Sabatini, D.D., Blobel, G., (1973) J. Cell Biol., 56, pp. 206-229

Citas:

---------- APA ----------
Idoyaga Vargas, V. & Carminatti, H. (1977) . Glycosylation of endogenous protein(s) of the rough and smooth microsomes by a lipid sugar intermediate. Molecular and Cellular Biochemistry, 16(2-3), 171-176.
http://dx.doi.org/10.1007/BF01732058
---------- CHICAGO ----------
Idoyaga Vargas, V., Carminatti, H. "Glycosylation of endogenous protein(s) of the rough and smooth microsomes by a lipid sugar intermediate" . Molecular and Cellular Biochemistry 16, no. 2-3 (1977) : 171-176.
http://dx.doi.org/10.1007/BF01732058
---------- MLA ----------
Idoyaga Vargas, V., Carminatti, H. "Glycosylation of endogenous protein(s) of the rough and smooth microsomes by a lipid sugar intermediate" . Molecular and Cellular Biochemistry, vol. 16, no. 2-3, 1977, pp. 171-176.
http://dx.doi.org/10.1007/BF01732058
---------- VANCOUVER ----------
Idoyaga Vargas, V., Carminatti, H. Glycosylation of endogenous protein(s) of the rough and smooth microsomes by a lipid sugar intermediate. Mol Cell Biochem. 1977;16(2-3):171-176.
http://dx.doi.org/10.1007/BF01732058