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Abstract:

Rabbit muscle phosphorylase b was found to be capable of forming protein bound ±-1,4 glucosyl chains upon incubation of the enzyme with appropriate concentrations of glucose-1-phosphate with no primer addition (unprimed synthesis). This activity would only be present in a small fraction of the total muscle phosphorylase b activity, as judged from the high concentrations of enzyme which are required to demonstrate the occurrence of unprimed synthesis. Polyacrylamide gel electrophoresis shows the presence of a phosphorylase isoenzyme capable of accepting glucosyl moieties, giving rise to a glucosylated protein enzymatically active in the chain lengthening of its own glucan. © 1977 Dr. W. Junk b.v. Publishers.

Registro:

Documento: Artículo
Título:A primer independent activity of rabbit muscle phosphorylase b
Autor:Tandecarz, J.; Lavintman, N.; Cardini, C.E.
Filiación:Instituto de Investigaciones Bioquímicas 'Fundación Campomar', and Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:glucose phosphate; glycogen phosphorylase b; in vitro study; muscle; theoretical study; Animal; Glycogen; Hydrogen-Ion Concentration; Kinetics; Muscles; Phosphorylases; Rabbits; Structure-Activity Relationship
Año:1977
Volumen:16
Número:2-3
Página de inicio:141
Página de fin:148
DOI: http://dx.doi.org/10.1007/BF01732055
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:glycogen phosphorylase b, 9012-69-5; Glycogen, 9005-79-2; Phosphorylases, EC 2.4.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p141_Tandecarz

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Citas:

---------- APA ----------
Tandecarz, J., Lavintman, N. & Cardini, C.E. (1977) . A primer independent activity of rabbit muscle phosphorylase b. Molecular and Cellular Biochemistry, 16(2-3), 141-148.
http://dx.doi.org/10.1007/BF01732055
---------- CHICAGO ----------
Tandecarz, J., Lavintman, N., Cardini, C.E. "A primer independent activity of rabbit muscle phosphorylase b" . Molecular and Cellular Biochemistry 16, no. 2-3 (1977) : 141-148.
http://dx.doi.org/10.1007/BF01732055
---------- MLA ----------
Tandecarz, J., Lavintman, N., Cardini, C.E. "A primer independent activity of rabbit muscle phosphorylase b" . Molecular and Cellular Biochemistry, vol. 16, no. 2-3, 1977, pp. 141-148.
http://dx.doi.org/10.1007/BF01732055
---------- VANCOUVER ----------
Tandecarz, J., Lavintman, N., Cardini, C.E. A primer independent activity of rabbit muscle phosphorylase b. Mol Cell Biochem. 1977;16(2-3):141-148.
http://dx.doi.org/10.1007/BF01732055