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Abstract:

The microsomal fraction of insects was found to contain an enzyme which transfers mannose from guanosine diphosphate mannose to an endogenous or exogenous insect lipid and to other acceptors such as dolichol monophosphate or ficaprenol monophosphate. This activity depended on the presence of Triton X-100 and magnesium ions, the optimal concentration of the latter being 10mM. The optimal temperature of the reaction was 25 °C and the maximal activity was obtained at pH 7.9. The mannolipid formed behaved as a monophosphodiester when chromatographed on DEAE-cellulose. Weak acid treatment of the product liberated mannose. Its behaviour both on thin layer and Sephadex G-150 chromatography would indicate the presence of a number of isoprenyl units similar to the dolichol and different from the ficaprenol derivative. Stability to phenol treatment indicated that the lipid fraction of the mannolipid is an ±-saturated polyprenol phosphate similar to dolichol monophosphate. © 1977 Dr. W. Junk b.v. Publishers.

Registro:

Documento: Artículo
Título:Enzymatic synthesis of polyprenol monophosphate mannose in insects
Autor:Belocopitow, E.; Marechal, L.R.; Quesada Allue, L.A.
Filiación:Instituto de Investigaciones Bioquimicas 'Fundación Campomar', and Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:dolichol phosphate; mannose; arthropod; in vitro study; microsome; polyprenyl phosphate; theoretical study; Animal; Cobalt; Diptera; Enzyme Activation; Hexosephosphates; Hexosyltransferases; Hydrogen-Ion Concentration; Kinetics; Larva; Magnesium; Manganese; Mannosyltransferases; Microsomes; Phospholipids; Polyethylene Glycols; Pupa; Temperature; Terpenes; Triatoma; Triatominae
Año:1977
Volumen:16
Número:2-3
Página de inicio:127
Página de fin:134
DOI: http://dx.doi.org/10.1007/BF01732053
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:dolichol phosphate, 12698-55-4, 34457-14-2; mannose, 31103-86-3, 3458-28-4; Cobalt, 7440-48-4; Hexosephosphates; Hexosyltransferases, EC 2.4.1.-; Magnesium, 7439-95-4; Manganese, 7439-96-5; Mannosyltransferases, EC 2.4.1.; Phospholipids; Polyethylene Glycols; Terpenes
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p127_Belocopitow

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Citas:

---------- APA ----------
Belocopitow, E., Marechal, L.R. & Quesada Allue, L.A. (1977) . Enzymatic synthesis of polyprenol monophosphate mannose in insects. Molecular and Cellular Biochemistry, 16(2-3), 127-134.
http://dx.doi.org/10.1007/BF01732053
---------- CHICAGO ----------
Belocopitow, E., Marechal, L.R., Quesada Allue, L.A. "Enzymatic synthesis of polyprenol monophosphate mannose in insects" . Molecular and Cellular Biochemistry 16, no. 2-3 (1977) : 127-134.
http://dx.doi.org/10.1007/BF01732053
---------- MLA ----------
Belocopitow, E., Marechal, L.R., Quesada Allue, L.A. "Enzymatic synthesis of polyprenol monophosphate mannose in insects" . Molecular and Cellular Biochemistry, vol. 16, no. 2-3, 1977, pp. 127-134.
http://dx.doi.org/10.1007/BF01732053
---------- VANCOUVER ----------
Belocopitow, E., Marechal, L.R., Quesada Allue, L.A. Enzymatic synthesis of polyprenol monophosphate mannose in insects. Mol Cell Biochem. 1977;16(2-3):127-134.
http://dx.doi.org/10.1007/BF01732053