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Abstract:

DEAE-cellulose column chromatography of Neurospora crassa soluble mycelial extracts leads to the resolution of three major protein kinase activity peaks designated PKI, PKII, and PKIII. PKII activity is stimulated by Ca2+ and Neurospora or brain calmodulin. Maximal stimulation was observed at 2 μM-free Ca2+ and 1 μg/ml of the modulator. The stimulatory effect of the Ca2+-calmodulin complex was blocked by EGTA and by some calmodulin antagonists such as phenothiazine drugs or compound 48/80. PKII phosphorylates different proteins, among which histone II-A at a low concentration and CDPKS, the synthetic peptide specific for Ca2+-calmodulin dependent protein kinases, are the best substrates. Some phosphorylation can be detected in the absence of any exogenous acceptor. PKII activity assayed in the presence of histone II-A or in the absence of exogenous phosphate acceptor (autophosphorylation) co-elute in a DEAE-cellulose column at 0.28 M NaCl. As result of the autophosphorylation reaction of the purified enzyme a main phosphorylated component of 70 kDa was resolved by SDS-polyacrylamide gel electrophoresis. It is possible that this component is an active part of this enzyme. © 1991 Kluwer Academic Publishers.

Registro:

Documento: Artículo
Título:Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa
Autor:Ulloa, R.M.; Torres, H.N.; Ochatt, C.M.; Téllez-Iñón, M.T.
Filiación:Instituto, de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Argentina
Facultad de Ciencias Exactas y Naturales Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:autophosphorylation; Ca2+; calmodulin; kinase; Neurospora crassa; protein kinase (calcium,calmodulin); Ca(2+) Calmodulin Dependent Protein Kinase; calcium; calmodulin; cyclic AMP; egtazic acid; fungal protein; protein kinase; protein kinase (calcium,calmodulin); article; autophosphorylation; neurospora crassa; nonhuman; priority journal; enzymology; gel chromatography; ion exchange chromatography; isolation and purification; Neurospora crassa; phosphorylation; polyacrylamide gel electrophoresis; protein processing; Ascomycota; Neurospora; Neurospora crassa; Ca(2+)-Calmodulin Dependent Protein Kinase; Calcium; Calmodulin; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cyclic AMP; Egtazic Acid; Electrophoresis, Polyacrylamide Gel; Fungal Proteins; Neurospora crassa; Phosphorylation; Protein Kinases; Protein Processing, Post-Translational; Support, Non-U.S. Gov't
Año:1991
Volumen:102
Número:2
Página de inicio:155
Página de fin:163
DOI: http://dx.doi.org/10.1007/BF00234573
Título revista:Molecular and Cellular Biochemistry
Título revista abreviado:Mol Cell Biochem
ISSN:03008177
CODEN:MCBIB
CAS:calcium, 7440-70-2; cyclic AMP, 60-92-4; egtazic acid, 67-42-5; protein kinase (calcium,calmodulin), 97350-82-8; protein kinase, 9026-43-1; Ca(2+)-Calmodulin Dependent Protein Kinase, EC 2.7.1.123; Calcium, 7440-70-2; Calmodulin; Cyclic AMP, 60-92-4; Egtazic Acid, 67-42-5; Fungal Proteins; Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v102_n2_p155_Ulloa

Referencias:

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Citas:

---------- APA ----------
Ulloa, R.M., Torres, H.N., Ochatt, C.M. & Téllez-Iñón, M.T. (1991) . Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa. Molecular and Cellular Biochemistry, 102(2), 155-163.
http://dx.doi.org/10.1007/BF00234573
---------- CHICAGO ----------
Ulloa, R.M., Torres, H.N., Ochatt, C.M., Téllez-Iñón, M.T. "Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa" . Molecular and Cellular Biochemistry 102, no. 2 (1991) : 155-163.
http://dx.doi.org/10.1007/BF00234573
---------- MLA ----------
Ulloa, R.M., Torres, H.N., Ochatt, C.M., Téllez-Iñón, M.T. "Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa" . Molecular and Cellular Biochemistry, vol. 102, no. 2, 1991, pp. 155-163.
http://dx.doi.org/10.1007/BF00234573
---------- VANCOUVER ----------
Ulloa, R.M., Torres, H.N., Ochatt, C.M., Téllez-Iñón, M.T. Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa. Mol Cell Biochem. 1991;102(2):155-163.
http://dx.doi.org/10.1007/BF00234573