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Abstract:

Human exposure to multiple pyrethroid insecticides may occur because of their broad use on crops and for residential pest control. To address the potential health risk from co-exposure to pyrethroids, it is important to understand their disposition and toxicity in target organs such as the brain, and surrogates such as the blood when administered as a mixture. The objective of this study was to assess the correlation between blood and brain concentrations of pyrethroids and neurobehavioral effects in the rat following an acute oral administration of the pyrethroids as a mixture. Male Long-Evans rats were administered a mixture of β-cyfluthrin, cypermethrin, deltamethrin, esfenvalerate and cis- and trans-permethrin in corn oil at seven dose levels. The pyrethroid with the highest percentage in the dosing solution was trans-permethrin (31% of total mixture dose) while deltamethrin and esfenvalerate had the lowest percentage (3%). Motor activity of the rats was then monitored for 1 h. At 3.5 h post-dosing, the animals were euthanized and blood and brain were collected. These tissues were extracted and analyzed for parent pyrethroid using HPLC-tandem mass spectrometry. Cypermethrin and cis-permethrin were the predominate pyrethroids detected in blood and brain, respectively, at all dosage levels. The relationship of total pyrethroid concentration between blood and brain was linear (r = 0.93). The pyrethroids with the lowest fraction in blood were trans-permethrin and β-cyfluthrin and in brain were deltamethrin and esfenvalerate. The relationship between motor activity of the treated rats and summed pyrethroid blood and brain concentration was described using a sigmoidal Emax model with the Effective Concentration50 being more sensitive for brain than blood. The data suggests summed pyrethroid rat blood concentration could be used as a surrogate for brain concentration as an aid to study the neurotoxic effects of pyrethroids administered as a mixture under the conditions used in this study. © 2016

Registro:

Documento: Artículo
Título:Environmentally relevant pyrethroid mixtures: A study on the correlation of blood and brain concentrations of a mixture of pyrethroid insecticides to motor activity in the rat
Autor:Hughes, M.F.; Ross, D.G.; Starr, J.M.; Scollon, E.J.; Wolansky, M.J.; Crofton, K.M.; DeVito, M.J.
Filiación:U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Research Triangle ParkNC, United States
U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, Research Triangle ParkNC, United States
National Research Council, Research Triangle ParkNC, United States
Syngenta Crop Protection, Greensboro, NC, United States
University of Buenos Aires and Argentine NRC Institute IQUIBICEN, Buenos Aires, Argentina
U.S. Environmental Protection Agency, Office of Research and Development, National Center for Computational Toxicology, Research Triangle ParkNC, United States
National Institute of Environmental Health Sciences, National Toxicology Program, Research Triangle ParkNC, United States
Palabras clave:Dosimetry; Mixture; Neurotoxicity; Pesticide; Pyrethroid; corn oil; cyfluthrin; cypermethrin; deltamethrin; fenvalerate; permethrin; pyrethroid; insecticide; pyrethroid; animal cell; animal euthanasia; animal experiment; Article; blood; blood sampling; brain; cognition; controlled study; drug blood level; drug brain level; drug megadose; drug mixture; EC50; high performance liquid chromatography; in vivo study; linear regression analysis; male; motor activity; neurotoxicity; nonhuman; priority journal; rat; tandem mass spectrometry; animal; blood; brain; drug effects; drug interaction; Long Evans rat; metabolism; motor activity; Animals; Brain; Drug Interactions; Insecticides; Male; Motor Activity; Pyrethrins; Rats; Rats, Long-Evans
Año:2016
Volumen:359-360
Página de inicio:19
Página de fin:28
DOI: http://dx.doi.org/10.1016/j.tox.2016.06.013
Título revista:Toxicology
Título revista abreviado:Toxicology
ISSN:0300483X
CODEN:TXCYA
CAS:corn oil, 8001-30-7; cyfluthrin, 68359-37-5; cypermethrin, 52315-07-8, 65731-84-2, 66841-24-5, 67375-30-8; deltamethrin, 52918-63-5; fenvalerate, 51630-58-1; permethrin, 51877-74-8, 52645-53-1; Insecticides; Pyrethrins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0300483X_v359-360_n_p19_Hughes

Referencias:

  • ATSDR, Public Health Statement. Pyrethrins and Pyrethroids. Agency for Toxic Substances and Disease Registry (2003), http://www.atscr.cdc.gov/ToxProfiles/tp155-c1-b.pdf, (accessed 06.03.14); Abernathy, C.O., Ueda, K., Engel, J.L., Gaughan, L.C., Casida, J.E., Substrate-specificity and toxicological significance of pyrethroid-hydrolyzing esterases of mouse liver microsomes (1973) Pestic. Biochem. Physiol., 3, pp. 300-311
  • Abu-Qare, A.W., Abou-Donia, M.B., Binding of pyridostigmine bromide, N,N-diethyl-m-toluamide and permethrin alone and in combinations, to human serum albumin (2002) Arch. Toxicol., 26, pp. 203-208
  • Amweg, E.L., Weston, D.P., Ureda, N.M., Use and toxicity of pyrethroid pesticides in the Central Valley California, USA (2005) Environ. Toxicol. Chem., 24, pp. 966-972
  • Bradberry, S.M., Cage, S.A., Proudfoot, A.T., Vale, J.A., Poisoning due to pyrethroids (2005) Toxicol. Rev., 24, pp. 93-106
  • Breckenridge, C.B., Holden, L., Sturgess, N., Weinder, M., Sheets, L., Evidence for separate mechanism of toxicity for the Type I and the Type II pyrethroid insecticides (2009) Neurotoxicology, 30S, pp. S17-S31
  • Brzezinski, M.R., Abraham, T.L., Stone, C.L., Dean, R.A., Bosron, W.F., Purification and characterization of a human liver cocaine carboxylesterase that catalyzed the production of benzoylecgonine and the formation of cocaethylene from alcohol and cocaine (1994) Biochem. Pharmacol., 48, pp. 1747-1755
  • Cao, Z., Shafer, T.J., Crofton, K.M., Gennings, C., Murray, T.F., Additivity of pyrethroid actions on sodium influx in cerebrocortical neurons in primary culture (2011) Environ. Health Perspect., 119, pp. 1239-1246
  • Crofton, K.M., Reiter, L.W., Effects of two pyrethroid insecticides on motor activity and the acoustic startle response in the rat (1984) Toxicol. Appl. Pharmacol., 15, pp. 318-328
  • Crofton, K.M., Reiter, L.W., The effects of type I and II pyrethroids on motor activity and the acoustic startle response in the rat (1988) Fundam. Appl. Toxicol., 10, pp. 624-634
  • Crow, J.A., Borazjani, A., Potter, P.M., Ross, M.K., Hydrolysis of pyrethroids by human and rat tissues: examination of intestinal: liver and serum carboxylesterases (2007) Toxicol. Appl. Pharmacol., 221, pp. 1-12
  • Fortin, M.-C., Bouchard, M., Carrier, G., Dumas, P., Biological monitoring of exposure to pyrethrins and pyrethroids in a metropolitan population of the Province of Quebec, Canada (2008) Environ. Res., 107, pp. 343-350
  • Godin, S.J., Scollon, E.J., Hughes, M.F., Potter, P.M., DeVito, M.J., Species differences in the in vitro metabolism of deltamethrin and esfenvalerate: differential oxidative and hydrolytic metabolism by humans and rats (2006) Drug Metab. Dispos., 34, pp. 1764-1771
  • Godin, S.J., Crow, J.A., Scollon, E.J., Hughes, M.F., DeVito, M.J., Identification of rat and human cytochrome P450 isoforms and a rat serum esterase that metabolize the pyrethroid insecticides deltamethrin and esfenvalerate (2007) Drug Metab. Dispos., 35, pp. 1664-1671
  • Godin, S.J., Devito, M.J., Hughes, M.F., Ross, D.G., Scollon, E.J., Physiologically based pharmacokinetic modeling of deltamethrin: development of a rat and human diffusion-limited model (2010) Toxicol. Sci., 115, pp. 330-343
  • Gray, A.J., Rickard, J., Toxicity of pyrethroids to rats after direct injection into the central nervous system (1982) Neurotoxicology, 3, pp. 25-35
  • Gray, A.J., Rickard, J., The toxicokinetics of deltamethrin in rats after intravenous administration of a toxic dose (1982) Pestic. Biochem. Physiol., 18, pp. 205-215
  • Heudorf, U., Angerer, J., Metabolites of pyrethroid insecticides in urine specimens: current exposure in an urban population in Germany (2001) Environ. Health Perspect., 109, pp. 213-217
  • Hosokawa, M., Structure and catalytic properties of carboxylesterase isozymes involved in the metabolic activation of prodrugs (2008) Molecules, 13, pp. 412-431
  • Lawrence, L.J., Casida, J.E., Pyrethroid toxicology: mouse intracerebral structure-activity relationships (1982) Pestic. Biochem. Physiol., 18, pp. 9-14
  • Meador, J.P., McCarty, L.S., Escher, B.I., Adams, W.U., 10th Anniversary Critical Review: the tissue-residue approach for toxicity assessment: concepts, issues, application, and recommendations (2008) J. Environ. Monit., 10, pp. 1486-1498
  • Mirfazaelian, A., Kim, K.-B., Anand, S.S., Kim, H.J., Tornero-Velez, R., Development of a physiologically based pharmacokinetic model for deltamethrin in the adult male Sprague-Dawley rat (2006) Toxicol. Sci., 92, pp. 432-442
  • Miyamoto, J., Stereoselective metabolism and toxicology of pyrethroids (1990) Chirality and Biological Activity, pp. 153-168. , B. Holmstedt H. Frank B. Testa A.R. Liss, Inc. New York
  • Nakamura, Y., Sugihara, K., Sone, T., Isobe, M., Ohta, S., The in vitro metabolism of a pyrethroid insecticide, permethrin and its hydrolysis products in rats (2007) Toxicology, 235, pp. 176-184
  • Nishi, K., Huang, H., Kamita, S.G., Kim, I.-H., Morisseau, C., Characterization of pyrethroid hydrolysis by the human liver carboxylesterases hCE-1 and hCE-2 (2006) Arch. Biochem. Biophys., 445, pp. 115-123
  • Okura, K., Tasaka, K., Hashimoto, M., Imai, T., Distinct patterns of aging effects and activity of carboxylesterase in rat liver and intestine (2014) Drug Metab. Dispos., 42, pp. 264-273
  • Pindel, E.V., Kedishvili, N.Y., Abraham, T.L., Brzezinski, M.R., Zhang, J., Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin (1997) J. Biol. Chem., 272, pp. 14769-14775
  • Pohl, P.C., Klafke, G.M., Junior, J.R., Martins, J.R., da Silva Vas, I., Jr., ABC transporters as a multidrug detoxification mechanism in Rhipicephalus (Boophilus) microplus (2012) Parasitol. Res., 111, pp. 2345-2351
  • Ross, M.K., Borazjani, A., Edwards, C.C., Potter, P.M., Hydrolytic metabolism of pyrethroids by human and other mammalian carboxylesterases (2006) Biochem. Pharmacol., 71, pp. 657-669
  • Rudel, R.A., Camann, D.E., Spengler, J.D., Korn, L.R., Brody, J.G., Phthalates, alkylphenols, pesticides, polybrominated diphenyl ethers, and other endocrine-disrupting compounds in indoor air and dust (2003) Environ. Sci. Technol., 72, pp. 4543-4553
  • Scollon, E.J., Starr, J.M., Godin, S.J., DeVito, M.J., Hughes, M.F., In vitro metabolism of pyrethroid pesticides by rat and human hepatic microsomes and cytochrome P450 isoforms (2009) Drug Metab. Dispos., 37, pp. 221-228
  • Scollon, E.J., Starr, J.M., Crofton, K.M., Wolansky, M.J., DeVito, M.J., Correlation of tissue concentrations of the pyrethroid bifenthrin with neurotoxicity in the rat (2011) Toxicology, 290, pp. 1-6
  • Sethi, P.K., Muralidhara, S., Bruckner, J.V., White, C.A., Measurement of plasma protein and lipoprotein binding of pyrethroids (2014) J. Pharmacol. Toxicol. Methods, 70, pp. 106-111
  • Soderlund, D.M., Clark, J., Sheets, L.P., Mullin, L.S., Piccirillo, V.J., Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment (2002) Toxicology, 17, pp. 3-59
  • Soderlund, D.M., Molecular mechanisms of pyrethroid insecticide neurotoxicity: recent advances (2012) Arch. Toxicol., 86, pp. 165-181
  • Starr, J.M., Scollon, E.J., Hughes, M.F., Ross, D.G., Graham, S.E., Environmentally relevant mixtures in cumulative assessments: an acute study of toxicokinetics and effects on motor activity in rats exposed to a mixture of pyrethroids (2012) Toxicol. Sci., 130, pp. 309-318
  • Starr, J.M., Graham, S.E., Ross, D.G., Tornero-Velez, R., Scollon, E.J., Environmentally relevant mixing ratios in cumulative assessments: a study of the kinetics of pyrethroids and their ester cleavage metabolites in blood and brain; and the effect of a pyrethroid mixture on the motor activity of rats (2014) Toxicology, 310, pp. 15-24
  • Stout, D.M., II, Bradham, K.D., Egeghy, P.P., Jones, P.A., Croghan, C.W., American Healthy Homes Survey: a national study of residential pesticides measured from floor wipes (2009) Environ. Sci. Technol., 43, pp. 4294-4300
  • Tornero-Velez, R., Egeghy, P.P., Cohen Hubal, E.A., Biogeographical analysis of chemical co-occurrence data to identify priorities for mixtures research (2012) Risk Anal., 32, pp. 224-236
  • Tulve, N.S., Jones, P.A., Nishioka, M., Fortmann, R.C., Croghan, C.W., Pesticide measurements from the first national environmental health survey of child care centers using a multi-residue GC/MS analysis method (2006) Environ. Sci. Technol., 40, pp. 6269-6274
  • US, E.P.A., Pyrethrins/Pyrethroid Cumulative Risk Assessment (2011), http://www.regulations.gov/#!documentDetail;D=EPA-HQ-OPP-2011-0746-0003, United States Environmental Protection Agency. . (accessed 15.01.16); http://www.ams.usda.gov/sites/default/files/media/2014%20PDP%20Annual%20Summary.pdf, USDA, 2014. Pesticide Data Program. Annual Summary. Calendar Year 2014. United States Department of Agriculture. (accessed 02.02.16); Wolansky, M.J., Gennings, C., Crofton, K.M., Relative potencies for acute effects of pyrethroids on motor function in rats (2006) Toxicol. Sci., 89, pp. 271-277
  • Wolansky, M.J., Gennings, C., DeVito, M.J., Crofton, K.M., Evidence for dose-additive effects of pyrethroids on motor activity in rats (2009) Environ. Health Perspect., 117, pp. 1563-1570
  • Zastre, J., Dowd, C., Bruckner, J., Popovici, A., Lack of P-glycoprotein-mediated efflux and the potential involvement of an influx transport process contributing to the intestinal uptake of deltamethrin cis-permethrin, and trans-permethrin (2013) Toxicol. Sci., 136, pp. 284-293

Citas:

---------- APA ----------
Hughes, M.F., Ross, D.G., Starr, J.M., Scollon, E.J., Wolansky, M.J., Crofton, K.M. & DeVito, M.J. (2016) . Environmentally relevant pyrethroid mixtures: A study on the correlation of blood and brain concentrations of a mixture of pyrethroid insecticides to motor activity in the rat. Toxicology, 359-360, 19-28.
http://dx.doi.org/10.1016/j.tox.2016.06.013
---------- CHICAGO ----------
Hughes, M.F., Ross, D.G., Starr, J.M., Scollon, E.J., Wolansky, M.J., Crofton, K.M., et al. "Environmentally relevant pyrethroid mixtures: A study on the correlation of blood and brain concentrations of a mixture of pyrethroid insecticides to motor activity in the rat" . Toxicology 359-360 (2016) : 19-28.
http://dx.doi.org/10.1016/j.tox.2016.06.013
---------- MLA ----------
Hughes, M.F., Ross, D.G., Starr, J.M., Scollon, E.J., Wolansky, M.J., Crofton, K.M., et al. "Environmentally relevant pyrethroid mixtures: A study on the correlation of blood and brain concentrations of a mixture of pyrethroid insecticides to motor activity in the rat" . Toxicology, vol. 359-360, 2016, pp. 19-28.
http://dx.doi.org/10.1016/j.tox.2016.06.013
---------- VANCOUVER ----------
Hughes, M.F., Ross, D.G., Starr, J.M., Scollon, E.J., Wolansky, M.J., Crofton, K.M., et al. Environmentally relevant pyrethroid mixtures: A study on the correlation of blood and brain concentrations of a mixture of pyrethroid insecticides to motor activity in the rat. Toxicology. 2016;359-360:19-28.
http://dx.doi.org/10.1016/j.tox.2016.06.013