Artículo

Randi, A.S.; Sancovich, H.A.; Ferramola De Sancovich, A.M.; Loaiza, A.; Krawiec, L.; Kleiman De Pisarev, D.L. "Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function" (1998) Toxicology. 125(2-3):83-94
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Abstract:

The time and dose-dependent effects of the in vivo administration of hexachlorobenzene (HCB), on hepatic microsomal membrane functions, were studied in female Wistar rats. Administration of HCB (100 mg/100 g b.w.) resulted in time-dependent decreases in the activity of two membrane-bound enzymes: 5'nucleotidase and Na+/K+ ATPase. HCB was found to cause a significant rise in protein tyrosine kinase (PTK) activity during the early stages of intoxication (day 2), followed by a significant decrease at 10 days, returning to control levels after 20 days of treatment. A stimulatory effect of HCB on in vitro endogenous microsomal protein phosphorylation was observed from 2 days of intoxication up to 30 days of treatment, with an important stimulation of phosphorylation at 5 days. Administration of HCB (100 mg/100 g b.w.) for 10 days caused a 50% reduction in epidermal growth factor receptor (EGF-R) ligand binding. The effects of known specific inhibitors of protein phosphatases on endogenous protein phosphorylation were studied. HCB affected the labelling of several bands, as well as the 5'nucleotidase and PTK activities, in a dose-dependent manner. In conclusion, this study indicated that the in vivo administration of HCB results in a significant alteration of membrane function.

Registro:

Documento: Artículo
Título:Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function
Autor:Randi, A.S.; Sancovich, H.A.; Ferramola De Sancovich, A.M.; Loaiza, A.; Krawiec, L.; Kleiman De Pisarev, D.L.
Filiación:Vidal 2483 8 'B' (1428), Dept. Hum. Biochem., Sch. Med., U., Buenos Aires, Argentina
Department of Biological Chemistry, Sch. of Sci., Univ. of Buenos Aires, Buenos Aires, Argentina
Department of Radiobiology, Nucl. Biochem., Natl. Commitee A., Buenos Aires, Argentina
Palabras clave:Epidermal growth factor receptor; Hexachlorobenzene; Mechanism of action; Membrane protein phosphorylation; Protein tyrosine kinase; epidermal growth factor receptor; hexachlorobenzene; protein tyrosine kinase; animal experiment; article; dose response; drug effect; enzyme activation; enzyme activity; female; liver microsome; liver toxicity; nonhuman; priority journal; protein phosphorylation; rat; 5'-Nucleotidase; Animals; Dose-Response Relationship, Drug; Epidermal Growth Factor; Female; Hexachlorobenzene; Intracellular Membranes; Microsomes, Liver; Na(+)-K(+)-Exchanging ATPase; Phosphorylation; Protein-Tyrosine Kinases; Rats; Rats, Wistar; Receptor, Epidermal Growth Factor; Soil Pollutants
Año:1998
Volumen:125
Número:2-3
Página de inicio:83
Página de fin:94
DOI: http://dx.doi.org/10.1016/S0300-483X(97)00165-0
Título revista:Toxicology
Título revista abreviado:Toxicology
ISSN:0300483X
CODEN:TXCYA
CAS:hexachlorobenzene, 118-74-1, 55600-34-5; protein tyrosine kinase, 80449-02-1; 5'-Nucleotidase, 3.1.3.5; Epidermal Growth Factor, 62229-50-9; Hexachlorobenzene, 118-74-1; Na(+)-K(+)-Exchanging ATPase, 3.6.1.37; Protein-Tyrosine Kinases, 2.7.1.112; Receptor, Epidermal Growth Factor, 2.7.1.112; Soil Pollutants
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0300483X_v125_n2-3_p83_Randi

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Citas:

---------- APA ----------
Randi, A.S., Sancovich, H.A., Ferramola De Sancovich, A.M., Loaiza, A., Krawiec, L. & Kleiman De Pisarev, D.L. (1998) . Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function. Toxicology, 125(2-3), 83-94.
http://dx.doi.org/10.1016/S0300-483X(97)00165-0
---------- CHICAGO ----------
Randi, A.S., Sancovich, H.A., Ferramola De Sancovich, A.M., Loaiza, A., Krawiec, L., Kleiman De Pisarev, D.L. "Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function" . Toxicology 125, no. 2-3 (1998) : 83-94.
http://dx.doi.org/10.1016/S0300-483X(97)00165-0
---------- MLA ----------
Randi, A.S., Sancovich, H.A., Ferramola De Sancovich, A.M., Loaiza, A., Krawiec, L., Kleiman De Pisarev, D.L. "Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function" . Toxicology, vol. 125, no. 2-3, 1998, pp. 83-94.
http://dx.doi.org/10.1016/S0300-483X(97)00165-0
---------- VANCOUVER ----------
Randi, A.S., Sancovich, H.A., Ferramola De Sancovich, A.M., Loaiza, A., Krawiec, L., Kleiman De Pisarev, D.L. Hexachlorobenzene-induced alterations of rat hepatic microsomal membrane function. Toxicology. 1998;125(2-3):83-94.
http://dx.doi.org/10.1016/S0300-483X(97)00165-0