Artículo

Cohen, S.; Petcoff, G.; Freijo, R.O.; Portiansky, E.L.; Barbeito, C.G.; Macchi, G.J.; Díaz, A.O. "Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)" (2015) Zoological Science. 32(4):383-388
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Abstract:

In the present study we histochemically and lectinhistochemically characterized the growing oocytes of the pink cuskeel (Genypterus blacodes). We used histochemical methods for the localization and characterization of glycoconjugates (GCs) and lectin histochemical techniques for the identification of specific sugar residues. We analyzed presence and distribution of GCs in the different structures of the growing follicles (cortical alveoli, globules, yolk granules and zona radiata). During the initial stage of vitellogenesis, the oocytes presented small yolk granules composed of GCs that gradually increased during exogenous vitellogenesis. These GCs contained moderate quantities of α-D-mannose, D-glucose, N-acetylglucosamine and N-acetyl-neuraminic acid. The cortical alveoli contained both neutral and carboxylated GCs, and lectin techniques detected N-acetylgalactosamine, galactose and L-fucose. The zona radiata showed a strong positive reaction to PAS and it reacted weakly with more specific techniques, such as KOH/PAS and PA/Bh/KOH/PAS. This structure showed GCs with oxidizable vicinal diols, O-acyl sugars and sialic acid residues with different substitution types and presented N-acetylgalactosamine and L-fucose specific residues. The oocytes follicular envelope evidenced neutral and acidic non-sulfated GCs and high concentrations of α-D-mannose, D-glucose, galactose and N-acetylgalactosamine. The intergranular cytoplasmic GCs were mainly rich in α-D-mannose, D-glucose, N-acetylgalactosamine, N-acetylglucosamine and N-acetyl-neuraminic acid. These results enhance the comprehension of the structure and functionality of the pink cuskeel ovarian follicles, and provide a useful tool for the study of this tissue in other teleost species. © 2015 Zoological Society of Japan.

Registro:

Documento: Artículo
Título:Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)
Autor:Cohen, S.; Petcoff, G.; Freijo, R.O.; Portiansky, E.L.; Barbeito, C.G.; Macchi, G.J.; Díaz, A.O.
Filiación:Instituto de Investigaciones Marinas y Costeras (IIMyC), FCEyN, CONICET, Universidad Nacional de Mar Del, Plata Funes 3250, Mar del Plata, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (Conicet), Argentina
Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar Del Plata, Argentina
Departamento de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata (UNLP), 60 y 118, La Plata, Argentina
Instituto de Patología, Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata (UNLP), 60 y 118, La Plata, Buenos Aires, Argentina
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Paseo Victoria Ocampo, 1 Mar del Plata, General Pueyrredón, Buenos Aires, Argentina
Palabras clave:Genypterus blacodes; Glycoconjugates; Histochemistry; Lectins; Oocytes; Genypterus blacodes; Radiata; Teleostei; animal; cytology; female; fish; oocyte; physiology; Animals; Female; Fishes; Oocytes
Año:2015
Volumen:32
Número:4
Página de inicio:383
Página de fin:388
DOI: http://dx.doi.org/10.2108/zs140235
Título revista:Zoological Science
Título revista abreviado:Zool. Sci.
ISSN:02890003
CODEN:ZOSCE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02890003_v32_n4_p383_Cohen

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Citas:

---------- APA ----------
Cohen, S., Petcoff, G., Freijo, R.O., Portiansky, E.L., Barbeito, C.G., Macchi, G.J. & Díaz, A.O. (2015) . Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes). Zoological Science, 32(4), 383-388.
http://dx.doi.org/10.2108/zs140235
---------- CHICAGO ----------
Cohen, S., Petcoff, G., Freijo, R.O., Portiansky, E.L., Barbeito, C.G., Macchi, G.J., et al. "Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)" . Zoological Science 32, no. 4 (2015) : 383-388.
http://dx.doi.org/10.2108/zs140235
---------- MLA ----------
Cohen, S., Petcoff, G., Freijo, R.O., Portiansky, E.L., Barbeito, C.G., Macchi, G.J., et al. "Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes)" . Zoological Science, vol. 32, no. 4, 2015, pp. 383-388.
http://dx.doi.org/10.2108/zs140235
---------- VANCOUVER ----------
Cohen, S., Petcoff, G., Freijo, R.O., Portiansky, E.L., Barbeito, C.G., Macchi, G.J., et al. Histochemical characterization of oocytes in the pink Cuskeel (Genypterus blacodes). Zool. Sci. 2015;32(4):383-388.
http://dx.doi.org/10.2108/zs140235