Abstract:
A galactofuranosyl-containing glycopeptide has been isolated from mycelium of Ascobolus furfuraceus by extraction with water. The glycoconjugate was purified by DEAE-cellulose chromatography followed by gel filtration. A molecular weight of about 20 000 was determined by the latter method using standard dextrans. Neutral sugars accounted for 94.5% of the glycopeptide and were characterized as mannose, galactose, and glucose. Glucosamine was estimated colorimetrically (1.8%). The molar ratio of Man:Gal:Glc:GlcNH2 was 68:32:16:2. A trace amount of total phosphorus (0.2%) was found. The predominant amino acids were threonine and serine. The peptide moiety was labeled with [14C]formaldehyde and the elution of radioactivity was coincident with sugar on gel filtration in the presence of sodium dodecyl sulfate. The peak of radioactivity was retarded on release of galactose by mild acid hydrolysis. These results confirm the sugar-peptide linkage. © 1986 Glycoconjugate Journal.
Registro:
Documento: |
Artículo
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Título: | Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus |
Autor: | Groisman, J.F.; Mercuri, O.A.; De Lederkremer, R.M. |
Filiación: | Departmento de Quimica Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina Departmento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
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Palabras clave: | Ascobolus furfuraceus; galactofuranosyl-containing glycopeptide; glycopeptide; beta galactofuranose; fungus; nonhuman |
Año: | 1986
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Volumen: | 3
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Número: | 1
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Página de inicio: | 45
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Página de fin: | 53
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DOI: |
http://dx.doi.org/10.1007/BF01108611 |
Título revista: | Glycoconjugate Journal
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Título revista abreviado: | Glycoconjugate J
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ISSN: | 02820080
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CODEN: | GLJOE
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02820080_v3_n1_p45_Groisman |
Referencias:
- Mercuri, O.A., Groisman, J.F., Lederkremer, R.M., (1984) An Asoc Quim Argent, 72, pp. 65-69
- Groisman, J.F., Forchiassin, F., Ranalli, M.E., Lederkremer, R.M., (1981) Exp Mycol, 5, pp. 253-57
- Gander, J.E., (1974) Annu Rev Microbiol, 28, pp. 103-19
- Gorin, P.A.J., Barreto-Bergter, E., (1983) Adv Carbohydr Chem Biochem, 41, pp. 67-103
- Gander, J.E., Jentoft, N.H., Drewes, L.R., Rick, P.D., (1974) J Biol Chem, 249, pp. 2063-72
- Rick, P.D., Drewes, L.R., Gander, J.E., (1974) J Biol Chem, 249, pp. 2073-78
- Unkefer, C.J., Jackson, C., Gander, J.E., (1982) J Biol Chem, 257, pp. 2491-97
- Herschlag, D., Stevens, E.S., Gander, J.E., (1983) Int J Pept Protein Res, 22, pp. 16-20
- Nakajima, T., Yoshida, M., Hiura, N., Matsuda, K., (1984) J Biochem (Tokyo), 96, pp. 1005-11
- Nakajima, T., Yoshida, M., Nakamura, M., Hiura, N., Matsuda, K., (1984) J Biochem (Tokyo), 96, pp. 1013-20
- Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., (1956) Anal Chem, 28, pp. 350-56
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951) J Biol Chem, 193, pp. 265-75
- Dittmer, J.C., Wells, M.A., (1969) Methods Enzymol, 14, pp. 482-530
- Davidson, E.A., (1966) Methods Enzymol, 8, pp. 52-60
- Trevelyan, W.E., Procter, D.P., Harrison, J.S., (1950) Nature, 166, pp. 444-45
- Hough L, Jones JKN, Wadman WH 1950 J Chem Soc 1702–6; Toennies, G., Kole, J.J., (1951) Anal Chem, 23, pp. 823-26
- Carminatti, H., Passeron, S., (1966) Methods Enzymol, 8, pp. 108-11
- Bray, G.A., (1960) Anal Biochem, 1, pp. 279-85
- Sloneker, J.H., (1972) Methods Carbohydr Chem, 6, pp. 20-24
- Weitzman, S., Scott, V., Keegstra, K., (1979) Anal Biochem, 97, pp. 438-49
- Segrest, J.P., Jackson, R.L., (1972) Methods Enzymol, 28, pp. 54-63
- Hsu, D.S., Hoffman, P., Mashburn, T.A., Jr, (1974) Biochem Biophys Acta, 338, pp. 254-64
- Rice, R.H., Means, G.E., (1971) J Biol Chem, 246, pp. 831-32
- MacFadyen, D.A., (1945) J Biol Chem, 158, pp. 107-33
- Rietschel-Berst, M., Jentoft, N.H., Rick, P.D., Pletcher, C., Fang, F., Gander, J.E., (1977) J Biol Chem, 252, pp. 3219-26
- Tarentino, A.L., Trimble, R.B., Maley, F., (1978) Methods Enzymol, 50, pp. 574-80
- Lloyd, K.O., (1970) Biochemistry, 9, pp. 3446-53
- Sentandreu, R., Northcote, D.H., (1968) Biochem J, 109, pp. 419-32
- Barker, S.A., Basarab, O., Cruickshank, C.N.D., (1967) Carbohydr Res, 3, pp. 325-32
- Bardalaye, P.C., Nordin, J.H., (1977) J Biol Chem, 252, pp. 2584-91
- Barreto-Bergter, E., Gorin, P.A.J., Travassos, L.R., (1981) Carbohydr Res, 95, pp. 205-18
- Mendonca-Previato, L., Gorin, P.A.J., Travassos, L.R., (1980) Infect Immun, 29, pp. 934-39
- Ukai, S., Matsuura, S., Hara, C., Kiho, T., Hirose, K., (1982) Carbohydr Res, 101, pp. 109-16
Citas:
---------- APA ----------
Groisman, J.F., Mercuri, O.A. & De Lederkremer, R.M.
(1986)
. Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus. Glycoconjugate Journal, 3(1), 45-53.
http://dx.doi.org/10.1007/BF01108611---------- CHICAGO ----------
Groisman, J.F., Mercuri, O.A., De Lederkremer, R.M.
"Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus"
. Glycoconjugate Journal 3, no. 1
(1986) : 45-53.
http://dx.doi.org/10.1007/BF01108611---------- MLA ----------
Groisman, J.F., Mercuri, O.A., De Lederkremer, R.M.
"Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus"
. Glycoconjugate Journal, vol. 3, no. 1, 1986, pp. 45-53.
http://dx.doi.org/10.1007/BF01108611---------- VANCOUVER ----------
Groisman, J.F., Mercuri, O.A., De Lederkremer, R.M. Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus. Glycoconjugate J. 1986;3(1):45-53.
http://dx.doi.org/10.1007/BF01108611