Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

A galactofuranosyl-containing glycopeptide has been isolated from mycelium of Ascobolus furfuraceus by extraction with water. The glycoconjugate was purified by DEAE-cellulose chromatography followed by gel filtration. A molecular weight of about 20 000 was determined by the latter method using standard dextrans. Neutral sugars accounted for 94.5% of the glycopeptide and were characterized as mannose, galactose, and glucose. Glucosamine was estimated colorimetrically (1.8%). The molar ratio of Man:Gal:Glc:GlcNH2 was 68:32:16:2. A trace amount of total phosphorus (0.2%) was found. The predominant amino acids were threonine and serine. The peptide moiety was labeled with [14C]formaldehyde and the elution of radioactivity was coincident with sugar on gel filtration in the presence of sodium dodecyl sulfate. The peak of radioactivity was retarded on release of galactose by mild acid hydrolysis. These results confirm the sugar-peptide linkage. © 1986 Glycoconjugate Journal.

Registro:

Documento: Artículo
Título:Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus
Autor:Groisman, J.F.; Mercuri, O.A.; De Lederkremer, R.M.
Filiación:Departmento de Quimica Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Departmento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Palabras clave:Ascobolus furfuraceus; galactofuranosyl-containing glycopeptide; glycopeptide; beta galactofuranose; fungus; nonhuman
Año:1986
Volumen:3
Número:1
Página de inicio:45
Página de fin:53
DOI: http://dx.doi.org/10.1007/BF01108611
Título revista:Glycoconjugate Journal
Título revista abreviado:Glycoconjugate J
ISSN:02820080
CODEN:GLJOE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02820080_v3_n1_p45_Groisman

Referencias:

  • Mercuri, O.A., Groisman, J.F., Lederkremer, R.M., (1984) An Asoc Quim Argent, 72, pp. 65-69
  • Groisman, J.F., Forchiassin, F., Ranalli, M.E., Lederkremer, R.M., (1981) Exp Mycol, 5, pp. 253-57
  • Gander, J.E., (1974) Annu Rev Microbiol, 28, pp. 103-19
  • Gorin, P.A.J., Barreto-Bergter, E., (1983) Adv Carbohydr Chem Biochem, 41, pp. 67-103
  • Gander, J.E., Jentoft, N.H., Drewes, L.R., Rick, P.D., (1974) J Biol Chem, 249, pp. 2063-72
  • Rick, P.D., Drewes, L.R., Gander, J.E., (1974) J Biol Chem, 249, pp. 2073-78
  • Unkefer, C.J., Jackson, C., Gander, J.E., (1982) J Biol Chem, 257, pp. 2491-97
  • Herschlag, D., Stevens, E.S., Gander, J.E., (1983) Int J Pept Protein Res, 22, pp. 16-20
  • Nakajima, T., Yoshida, M., Hiura, N., Matsuda, K., (1984) J Biochem (Tokyo), 96, pp. 1005-11
  • Nakajima, T., Yoshida, M., Nakamura, M., Hiura, N., Matsuda, K., (1984) J Biochem (Tokyo), 96, pp. 1013-20
  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., (1956) Anal Chem, 28, pp. 350-56
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951) J Biol Chem, 193, pp. 265-75
  • Dittmer, J.C., Wells, M.A., (1969) Methods Enzymol, 14, pp. 482-530
  • Davidson, E.A., (1966) Methods Enzymol, 8, pp. 52-60
  • Trevelyan, W.E., Procter, D.P., Harrison, J.S., (1950) Nature, 166, pp. 444-45
  • Hough L, Jones JKN, Wadman WH 1950 J Chem Soc 1702–6; Toennies, G., Kole, J.J., (1951) Anal Chem, 23, pp. 823-26
  • Carminatti, H., Passeron, S., (1966) Methods Enzymol, 8, pp. 108-11
  • Bray, G.A., (1960) Anal Biochem, 1, pp. 279-85
  • Sloneker, J.H., (1972) Methods Carbohydr Chem, 6, pp. 20-24
  • Weitzman, S., Scott, V., Keegstra, K., (1979) Anal Biochem, 97, pp. 438-49
  • Segrest, J.P., Jackson, R.L., (1972) Methods Enzymol, 28, pp. 54-63
  • Hsu, D.S., Hoffman, P., Mashburn, T.A., Jr, (1974) Biochem Biophys Acta, 338, pp. 254-64
  • Rice, R.H., Means, G.E., (1971) J Biol Chem, 246, pp. 831-32
  • MacFadyen, D.A., (1945) J Biol Chem, 158, pp. 107-33
  • Rietschel-Berst, M., Jentoft, N.H., Rick, P.D., Pletcher, C., Fang, F., Gander, J.E., (1977) J Biol Chem, 252, pp. 3219-26
  • Tarentino, A.L., Trimble, R.B., Maley, F., (1978) Methods Enzymol, 50, pp. 574-80
  • Lloyd, K.O., (1970) Biochemistry, 9, pp. 3446-53
  • Sentandreu, R., Northcote, D.H., (1968) Biochem J, 109, pp. 419-32
  • Barker, S.A., Basarab, O., Cruickshank, C.N.D., (1967) Carbohydr Res, 3, pp. 325-32
  • Bardalaye, P.C., Nordin, J.H., (1977) J Biol Chem, 252, pp. 2584-91
  • Barreto-Bergter, E., Gorin, P.A.J., Travassos, L.R., (1981) Carbohydr Res, 95, pp. 205-18
  • Mendonca-Previato, L., Gorin, P.A.J., Travassos, L.R., (1980) Infect Immun, 29, pp. 934-39
  • Ukai, S., Matsuura, S., Hara, C., Kiho, T., Hirose, K., (1982) Carbohydr Res, 101, pp. 109-16

Citas:

---------- APA ----------
Groisman, J.F., Mercuri, O.A. & De Lederkremer, R.M. (1986) . Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus. Glycoconjugate Journal, 3(1), 45-53.
http://dx.doi.org/10.1007/BF01108611
---------- CHICAGO ----------
Groisman, J.F., Mercuri, O.A., De Lederkremer, R.M. "Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus" . Glycoconjugate Journal 3, no. 1 (1986) : 45-53.
http://dx.doi.org/10.1007/BF01108611
---------- MLA ----------
Groisman, J.F., Mercuri, O.A., De Lederkremer, R.M. "Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus" . Glycoconjugate Journal, vol. 3, no. 1, 1986, pp. 45-53.
http://dx.doi.org/10.1007/BF01108611
---------- VANCOUVER ----------
Groisman, J.F., Mercuri, O.A., De Lederkremer, R.M. Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus. Glycoconjugate J. 1986;3(1):45-53.
http://dx.doi.org/10.1007/BF01108611