Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Affinity chromatography on Concanavalin-A Sepharose, followed by gel filtration and hydrophobic interaction chromatography, permits the isolation of low molecular weight N-glycosidically linked oligomannosidic glycopeptides (MGp) from the autoproteolysis products of human seminal plasma. The monosaccharide composition of MGp showed only mannose, N-acetylglucosamine and a small amount of galactose. Structural studies were carried out by methylation analysis and chromium trioxide oxidation, and results were consistent with the structures accepted for high-mannose N-glycans. MGp was capable of inhibiting the sperm acrosomal exocytosis mediated by sperm- surface receptors. These data suggest that MGp act as a 'decapacitation' factor preventing premature sperm exocytosis.

Registro:

Documento: Artículo
Título:Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
Autor:Lopes, C.H.G.L.; Mazzini, M.N.; Tortorella, H.; Konrath, R.A.; Brandelli, A.
Filiación:Departamento de Bioquímica, Instituto de Biociências, Univ. Federal do Rio Grande do Sul, 90050-170 Porto Alegre, Brazil
Depto. de Química Organica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Depto. de Cie. dos Alimentos, ICTA, Univ. Federal do Rio Grande do Sul, 91501 970 Porto Alegre, Brazil
ICTA-UFRGS, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, Brazil
Palabras clave:Carbohydrate; Exocytosis; Glycoconjugate; Human; Semen; Sperm; glycopeptide; mannose; acrosome; affinity chromatography; article; carbohydrate analysis; exocytosis; human; male; normal human; priority journal; seminal plasma; Acrosome Reaction; Carbohydrate Conformation; Chromatography, Affinity; Chromatography, Gas; Chromatography, Gel; Exocytosis; Glycopeptides; Humans; Male; Mannose; Oligosaccharides; Semen
Año:1998
Volumen:15
Número:5
Página de inicio:477
Página de fin:481
DOI: http://dx.doi.org/10.1023/A:1006982919464
Título revista:Glycoconjugate Journal
Título revista abreviado:Glycoconjugate J.
ISSN:02820080
CODEN:GLJOE
CAS:Glycopeptides; Mannose, 31103-86-3; Oligosaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02820080_v15_n5_p477_Lopes

Referencias:

  • Macek, M.B., Shur, B.D., (1988) Gamete Res, 20, pp. 93-109
  • Myles, D.G., Primakoff, P., (1991) Ann NY Acad Sci, 637, pp. 486-493
  • Tesarik, J., (1989) Hum Reprod, 4, pp. 957-961
  • Eng, L.A., Oliphant, G., (1978) Biol Reprod, 19, pp. 1083-1094
  • Fraser, L.R., (1984) J Reprod Fertil, 72, pp. 373-384
  • Audhya, T., Jakkadi, R., Zaneveld, L.J.D., (1987) Biol Reprod, 36, pp. 511-521
  • Mann, T., Lutwak-Mann, C., (1981) Male Reproductive Function and Semen, , Berlin: Springer-Verlag
  • Tortorella, H., Cerezo, J.M.S., Mazzini, M.N., Cerezo, A.S., (1983) Am J Reprod Immunol, 4, pp. 76-82
  • DiGiacomo, M.A., Montes, J.M., Mazzini, M.N., Cerezo, A.S., (1986) Acta Biochim Clin Latinoam, 20, pp. 123-128
  • Mori, K., Daitoh, T., Irahara, M., Kamada, M., Aono, T., (1989) Am J Obstet Gynecol, 161, pp. 207-211
  • Miranda, P.V., Echeverria, F.G., Briggiler, C.I.M., Brandelli, A., Blaquier, J.A., Tezon, J.G., (1997) Mol Hum Reprod, 3, pp. 399-404
  • Tesarik, J., Mendoza, C., Carreras, A., (1991) Fertil Steril, 56, pp. 113-118
  • Brandelli, A., Miranda, P.V., Tezon, J.G., (1994) Biochim Biophys Acta, 1220, pp. 299-304
  • Benoff, S., Cooper, G.W., Hurley, I., Napolitano, B., Rosenfeld, D.L., Scholl, G.M., Hershlag, A., (1993) Fertil Steril, 59, pp. 854-862
  • Mazzini, M.N., Di Giacomo, M.A., Cerezo, A.S., (1987) Biomed Chromatogr, 2, pp. 152-155
  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., (1956) Anal Chem, 28, pp. 350-356
  • Mendoza, C., Carreras, A., Moos, J., Tesarik, J., (1992) J Reprod Fertil, 95, pp. 755-763
  • Reinhold, V.N., (1972) Methods Enzymol, 25, pp. 244-249
  • Dmitriev, B.A., Backinowsky, L.V., Lvov, V.L., Knivel, Y.A., Kochetkov, N.K., Khomenko, N.A., (1975) Carbohydrate Res, 41, pp. 329-333
  • Sandford, P.A., Conrad, H.E., (1966) Biochemistry, 5, pp. 1508-1517
  • Stellner, K., Saito, H., Hakomori, S., (1973) Arch Biochem Biophys, 155, pp. 464-472
  • Mazzini, M.N., Cerezo, A.S., Cerezo, J.M.S., (1979) J Chromatogr, 173, pp. 365-371
  • Montreuil, J., (1984) Biol Cell, 51, pp. 115-132
  • Krusius, T., (1976) FEBS Lett, 66, pp. 86-89
  • Cohen, R.E., Ballou, C.E., (1980) Biochemistry, 19, pp. 4345-4358
  • Montreuil, J., Bouquelet, S., Debray, H., Fournet, B., Spik, B., Strecker, G., (1986) Carbohydrate Analysis, pp. 143-204. , Chaplin MF, Kennedy JF, eds Oxford: IRL Press
  • Sharon, N., Lis, H., (1989) Science, 246, pp. 227-234

Citas:

---------- APA ----------
Lopes, C.H.G.L., Mazzini, M.N., Tortorella, H., Konrath, R.A. & Brandelli, A. (1998) . Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma. Glycoconjugate Journal, 15(5), 477-481.
http://dx.doi.org/10.1023/A:1006982919464
---------- CHICAGO ----------
Lopes, C.H.G.L., Mazzini, M.N., Tortorella, H., Konrath, R.A., Brandelli, A. "Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma" . Glycoconjugate Journal 15, no. 5 (1998) : 477-481.
http://dx.doi.org/10.1023/A:1006982919464
---------- MLA ----------
Lopes, C.H.G.L., Mazzini, M.N., Tortorella, H., Konrath, R.A., Brandelli, A. "Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma" . Glycoconjugate Journal, vol. 15, no. 5, 1998, pp. 477-481.
http://dx.doi.org/10.1023/A:1006982919464
---------- VANCOUVER ----------
Lopes, C.H.G.L., Mazzini, M.N., Tortorella, H., Konrath, R.A., Brandelli, A. Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma. Glycoconjugate J. 1998;15(5):477-481.
http://dx.doi.org/10.1023/A:1006982919464