Artículo

Agüero, M.B.; Svetaz, L.; Sánchez, M.; Luna, L.; Lima, B.; López, M.L.; Zacchino, S.; Palermo, J.; Wunderlin, D.; Feresin, G.E.; Tapia, A. "Argentinean Andean propolis associated with the medicinal plant Larrea nitida Cav. (Zygophyllaceae). HPLC-MS and GC-MS characterization and antifungal activity" (2011) Food and Chemical Toxicology. 49(9):1970-1978
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Abstract:

The chemical profile and botanical origin of Andean Argentinian propolis were studied by HPLC-ESI-MS/MS and GC-MS techniques as well as the antifungal activity according to CLSI protocols. Dermatophytes and yeasts tested were strongly inhibited by propolis extracts (MICs between 31.25 and 125 μg/mL). The main antifungal compounds were: 3'methyl-nordihydroguaiaretic acid (MNDGA) 1, nordihydroguaiaretic acid (NDGA) 2 and a NDGA derivative 3, showing strong activity against Trichophyton mentagrophytes, T. rubrum and Microsporum gypseum (MICs between 15.6 and 31.25 μg/mL). The lignans 1 and 2 showed activities against clinical isolates of Candidas spp., Cryptococcus spp., T. rubrum and T. mentagrophytes (MICs and MFCs between 31.25 and 62.5 μg/mL). The lignan and volatile organic compounds (VOCs) profiles from propolis matched with those of exudates of Larrea nitida providing strong evidences on its botanical origin. These results support that Argentinian Andean propolis are a valuable natural product with potential to improve human health. Six compounds (1-6) were isolated from propolis for the first time, while compounds 1 and 3-6 were reported for first time as constituents of L. nitida Cav. © 2011 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Argentinean Andean propolis associated with the medicinal plant Larrea nitida Cav. (Zygophyllaceae). HPLC-MS and GC-MS characterization and antifungal activity
Autor:Agüero, M.B.; Svetaz, L.; Sánchez, M.; Luna, L.; Lima, B.; López, M.L.; Zacchino, S.; Palermo, J.; Wunderlin, D.; Feresin, G.E.; Tapia, A.
Filiación:Instituto de Biotecnología - Instituto de Ciencias Básicas, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), 5400 San Juan, Argentina
Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional del Rosario, Suipacha 531, CP 2000, Rosario, Argentina
UMYMFOR, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Piso 3, Pabellón 2, (1428), Buenos Aires, Argentina
Universidad Nacional de Córdoba-CONICET, Facultad Ciencias Químicas-Dpto. Química Orgánica-ISIDSA-CIBICI, Medina Allende esq. Haya de La Torre, Ciudad Universitaria, 5000 Córdoba, Argentina
Palabras clave:Antifungal activity; Argentinian Andean propolis; GC-MS; HPLC-ESI-MS/MS; Lignans; VOCs; 3' methyl nordihydroguaiaretic acid; 3,3',4' trihydroxy 4 methoxy 7,7' epoxylignan; 3,4,3',4' tetrahydroxy 7,7' epoxylignan; acetic acid ethyl ester; dichloromethane; lignan; nordihydroguaiaretic acid; nordihydroguaiaretic acid derivative; phenol derivative; plant medicinal product; propolis; unclassified drug; volatile organic compound; Argentina; Arthroderma gypseum; article; Candida; controlled study; Cryptococcus; dermatophyte; drug concentration; drug determination; drug isolation; drug screening; drug structure; fungal strain; fungicidal activity; high performance liquid chromatography; Larrea; Larrea nitida; mass fragmentography; minimum fungicidal concentration; minimum inhibitory concentration; nonhuman; Trichophyton mentagrophytes; Trichophyton rubrum; Antifungal Agents; Chromatography, High Pressure Liquid; Gas Chromatography-Mass Spectrometry; Larrea; Magnetic Resonance Spectroscopy; Mass Spectrometry; Microbial Sensitivity Tests; Propolis; Arthroderma benhamiae; Arthroderma gypseum; Arthrodermataceae; Larrea nitida; Zygophyllaceae
Año:2011
Volumen:49
Número:9
Página de inicio:1970
Página de fin:1978
DOI: http://dx.doi.org/10.1016/j.fct.2011.05.008
Título revista:Food and Chemical Toxicology
Título revista abreviado:Food Chem. Toxicol.
ISSN:02786915
CODEN:FCTOD
CAS:acetic acid ethyl ester, 141-78-6; dichloromethane, 75-09-2; nordihydroguaiaretic acid, 500-38-9; propolis, 8012-89-3; Antifungal Agents; Propolis, 9009-62-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02786915_v49_n9_p1970_Aguero

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Citas:

---------- APA ----------
Agüero, M.B., Svetaz, L., Sánchez, M., Luna, L., Lima, B., López, M.L., Zacchino, S.,..., Tapia, A. (2011) . Argentinean Andean propolis associated with the medicinal plant Larrea nitida Cav. (Zygophyllaceae). HPLC-MS and GC-MS characterization and antifungal activity. Food and Chemical Toxicology, 49(9), 1970-1978.
http://dx.doi.org/10.1016/j.fct.2011.05.008
---------- CHICAGO ----------
Agüero, M.B., Svetaz, L., Sánchez, M., Luna, L., Lima, B., López, M.L., et al. "Argentinean Andean propolis associated with the medicinal plant Larrea nitida Cav. (Zygophyllaceae). HPLC-MS and GC-MS characterization and antifungal activity" . Food and Chemical Toxicology 49, no. 9 (2011) : 1970-1978.
http://dx.doi.org/10.1016/j.fct.2011.05.008
---------- MLA ----------
Agüero, M.B., Svetaz, L., Sánchez, M., Luna, L., Lima, B., López, M.L., et al. "Argentinean Andean propolis associated with the medicinal plant Larrea nitida Cav. (Zygophyllaceae). HPLC-MS and GC-MS characterization and antifungal activity" . Food and Chemical Toxicology, vol. 49, no. 9, 2011, pp. 1970-1978.
http://dx.doi.org/10.1016/j.fct.2011.05.008
---------- VANCOUVER ----------
Agüero, M.B., Svetaz, L., Sánchez, M., Luna, L., Lima, B., López, M.L., et al. Argentinean Andean propolis associated with the medicinal plant Larrea nitida Cav. (Zygophyllaceae). HPLC-MS and GC-MS characterization and antifungal activity. Food Chem. Toxicol. 2011;49(9):1970-1978.
http://dx.doi.org/10.1016/j.fct.2011.05.008