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Castro, G.R.; Santopietro, L.M.D.; Siñeriz, F. "Acid Pullulanase from Bacillus polymyxa MIR-23" (1992) Applied Biochemistry and Biotechnology. 37(3):227-233
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Abstract:

Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identified as Bacillus polymyxa. The organism could grow in acidic conditions (pH 5.0) on a starch medium and produce α-amylase, pullulanase, and α-glucosidase. Batch cultures showed the highest enzyme activities in the stationary phase. Pullulanase activity exhibited an optimal temperature of 52-57°C at pH 4.5-5.5. These properties would allow its use in the saccharification processes in the starch industries. © 1993 The Humana Press Inc.

Registro:

Documento: Artículo
Título:Acid Pullulanase from Bacillus polymyxa MIR-23
Autor:Castro, G.R.; Santopietro, L.M.D.; Siñeriz, F.
Filiación:Cátedra de Microbiologia Superior, Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, PROIMI-MIRCEN. Avda. Belgrano y Caseros, Tucumán, 4000
Laboratorio de Microbiologia, Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:α-glucosidase; acidophilic microorganism; Bacillus polymyxa; batch culture; pullulanase, α-amylase; starch degradation
Año:1992
Volumen:37
Número:3
Página de inicio:227
Página de fin:233
DOI: http://dx.doi.org/10.1007/BF02788874
Título revista:Applied Biochemistry and Biotechnology
Título revista abreviado:Appl Biochem Biotechnol
ISSN:02732289
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02732289_v37_n3_p227_Castro

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Citas:

---------- APA ----------
Castro, G.R., Santopietro, L.M.D. & Siñeriz, F. (1992) . Acid Pullulanase from Bacillus polymyxa MIR-23. Applied Biochemistry and Biotechnology, 37(3), 227-233.
http://dx.doi.org/10.1007/BF02788874
---------- CHICAGO ----------
Castro, G.R., Santopietro, L.M.D., Siñeriz, F. "Acid Pullulanase from Bacillus polymyxa MIR-23" . Applied Biochemistry and Biotechnology 37, no. 3 (1992) : 227-233.
http://dx.doi.org/10.1007/BF02788874
---------- MLA ----------
Castro, G.R., Santopietro, L.M.D., Siñeriz, F. "Acid Pullulanase from Bacillus polymyxa MIR-23" . Applied Biochemistry and Biotechnology, vol. 37, no. 3, 1992, pp. 227-233.
http://dx.doi.org/10.1007/BF02788874
---------- VANCOUVER ----------
Castro, G.R., Santopietro, L.M.D., Siñeriz, F. Acid Pullulanase from Bacillus polymyxa MIR-23. Appl Biochem Biotechnol. 1992;37(3):227-233.
http://dx.doi.org/10.1007/BF02788874