Abstract:
Mab B21 is a monoclonal antibody (Mab) that recognizes an epithelial tumor surface antigen (ETSA-B21) from diverse human tumor cell lines including breast, ovary, uterus, and their cognate carcinoma tissues. A lower reactivity has been observed in normal breast tissue and benign hyperplesia. In this study, the characteristics of the ETSA-B21 antigen have been examined in greater detail in the MCF-7, SK-BR-3, and MDA-MB-453 breast cancer cell lines. Treatment with phosphatidylinositol-phospholipase C, but no neuraminidase were found to partially remove the ETSA-B21 signal from the cell surface as revealed by immunofluorescence microscopy. Inhibition of the N-glycosylation pathway by tunicamycin resulted in a decreased ETSA-B21 signal on the cell membrane. In addition, the antigen-antibody complex was internalized in breast cancer cells as demonstrated by an acidic wash internalization assay evaluated using immunofluorescence. In conclusion, this study suggests that ETSA-B21 is a GPI anchor N-glycosylated protein promoting specific antibody internalization in breast cancer cells.
Registro:
Documento: |
Artículo
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Título: | Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21 |
Autor: | Annibali, N.V.; Baldi, A. |
Filiación: | Institute of Biology and Experimental Medicine, National Research Council of Argentina, Obligado 2490, 1428 Buenos Aires, Argentina Institute of Biology and Experimental Medicine, Obligado 2490, 1428 Buenos Aires, Argentina
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Palabras clave: | glycoprotein; glycosylphosphatidylinositol; membrane antigen; monoclonal antibody; phosphatidylinositol; phospholipase C; sialidase; tumor antigen; tunicamycin; antigen antibody complex; antigen detection; antigen expression; article; breast cancer; breast hyperplasia; cancer cell culture; controlled study; human; human cell; immunofluorescence microscopy; internalization; priority journal; protein glycosylation; signal transduction; Mink cell focus-forming virus |
Año: | 1997
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Volumen: | 16
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Número: | 2
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Página de inicio: | 139
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Página de fin: | 145
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DOI: |
http://dx.doi.org/10.1089/hyb.1997.16.139 |
Título revista: | Hybridoma
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Título revista abreviado: | HYBRIDOMA
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ISSN: | 0272457X
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CODEN: | HYBRD
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CAS: | phospholipase C, 9001-86-9; sialidase, 9001-67-6; tunicamycin, 11089-65-9
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0272457X_v16_n2_p139_Annibali |
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Citas:
---------- APA ----------
Annibali, N.V. & Baldi, A.
(1997)
. Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21. Hybridoma, 16(2), 139-145.
http://dx.doi.org/10.1089/hyb.1997.16.139---------- CHICAGO ----------
Annibali, N.V., Baldi, A.
"Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21"
. Hybridoma 16, no. 2
(1997) : 139-145.
http://dx.doi.org/10.1089/hyb.1997.16.139---------- MLA ----------
Annibali, N.V., Baldi, A.
"Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21"
. Hybridoma, vol. 16, no. 2, 1997, pp. 139-145.
http://dx.doi.org/10.1089/hyb.1997.16.139---------- VANCOUVER ----------
Annibali, N.V., Baldi, A. Characterization of the ETSA-21 antigen, a glycosylphosphatidyl-inositol anchor glycoprotein identified in breast cancer cells using monoclonal antibody B21. HYBRIDOMA. 1997;16(2):139-145.
http://dx.doi.org/10.1089/hyb.1997.16.139