Artículo

Meyer, M.; Gonzalez Deniselle, M.C.; Garay, L.I.; Monachelli, G.G.; Lima, A.; Roig, P.; Guennoun, R.; Schumacher, M.; De Nicola, A.F. "Stage dependent effects of progesterone on motoneurons and glial cells of wobbler mouse spinal cord degeneration" (2010) Cellular and Molecular Neurobiology. 30(1):123-135
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Abstract:

In the Wobbler mouse, a mutation in the Vps54 gene is accompanied by motoneuron degeneration and astrogliosis in the cervical spinal cord. Previous work has shown that these abnormalities are greatly attenuated by progesterone treatment of clinically afflicted Wobblers. However, whether progesterone is effective at all disease stages has not yet been tested. The present work used genotyped (wr/wr) Wobbler mice at three periods of the disease: early progressive (1-2 months), established (5-8 months) or late stages (12 months) and age-matched wildtype controls (NFR/NFR), half of which were implanted with a progesterone pellet (20 mg) for 18 days. In untreated Wobblers, degenerating vacuolated motoneurons were initially abundant, experienced a slight reduction at the established stage and dramatically diminished during the late period. In motoneurons, the cholinergic marker choline acetyltransferase (ChAT) was reduced at all stages of the Wobbler disease, whereas hyperexpression of the growth-associated protein (GAP43) mRNA preferentially occurred at the early progressive and established stages. Progesterone therapy significantly reduced motoneuron vacuolation, enhanced ChAT immunoreactive perikarya and reduced the hyperexpression of GAP43 during the early progressive and established stages. At all stage periods, untreated Wobblers showed high density of glial fibrillary acidic protein (GFAP)+ astrocytes and decreased number of glutamine synthase (GS) immunostained cells. Progesterone treatment down-regulated GFAP+ astrocytes and up-regulated GS+ cell number. These data reinforced the usefulness of progesterone to improve motoneuron and glial cell abnormalities of Wobbler mice and further showed that therapeutic benefit seems more effective at the early progressive and established periods, rather than on advance stages of spinal cord neurodegeneration. © 2009 Springer Science+Business Media, LLC.

Registro:

Documento: Artículo
Título:Stage dependent effects of progesterone on motoneurons and glial cells of wobbler mouse spinal cord degeneration
Autor:Meyer, M.; Gonzalez Deniselle, M.C.; Garay, L.I.; Monachelli, G.G.; Lima, A.; Roig, P.; Guennoun, R.; Schumacher, M.; De Nicola, A.F.
Filiación:Instituto de Biologia y Medicina Experimental, CONICET, Laboratorio de Bioquimica Neuroendócrina, Obligado 2490, Buenos Aires 1428, Argentina
Department of Human Biochemistry, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
UMR788 Inserm and University Paris-Sud 11, Kremlin-Bicêtre, France
Palabras clave:Glial fibrillary acidic protein; Glutamine synthetase; Neurodegeneration; Neuroprotection; Progesterone; Wobbler; choline acetyltransferase; glial fibrillary acidic protein; glutamate ammonia ligase; messenger RNA; neuromodulin; progesterone; animal cell; animal experiment; animal model; animal tissue; article; cell degeneration; chronotherapy; controlled study; disease course; drug efficacy; female; genotype; glia cell; male; mouse; mouse mutant; neuroprotection; nonhuman; priority journal; protein expression; spinal cord atrophy; spinal cord motoneuron; wild type; wobbler mouse; Animals; Anterior Horn Cells; Astrocytes; Cell Count; Choline O-Acetyltransferase; Disease Models, Animal; Female; GAP-43 Protein; Gene Expression Regulation; Genotype; Glial Fibrillary Acidic Protein; Glutamate-Ammonia Ligase; Image Processing, Computer-Assisted; Male; Mice; Mice, Neurologic Mutants; Motor Neurons; Neuroglia; Progesterone; RNA, Messenger; Spinal Cord; Spinal Cord Diseases; Mus
Año:2010
Volumen:30
Número:1
Página de inicio:123
Página de fin:135
DOI: http://dx.doi.org/10.1007/s10571-009-9437-8
Título revista:Cellular and Molecular Neurobiology
Título revista abreviado:Cell. Mol. Neurobiol.
ISSN:02724340
CODEN:CMNED
CAS:choline acetyltransferase, 9012-78-6; glutamate ammonia ligase, 9023-70-5; progesterone, 57-83-0; Choline O-Acetyltransferase, 2.3.1.6; GAP-43 Protein; Glial Fibrillary Acidic Protein; Glutamate-Ammonia Ligase, 6.3.1.2; Progesterone, 57-83-0; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v30_n1_p123_Meyer

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Citas:

---------- APA ----------
Meyer, M., Gonzalez Deniselle, M.C., Garay, L.I., Monachelli, G.G., Lima, A., Roig, P., Guennoun, R.,..., De Nicola, A.F. (2010) . Stage dependent effects of progesterone on motoneurons and glial cells of wobbler mouse spinal cord degeneration. Cellular and Molecular Neurobiology, 30(1), 123-135.
http://dx.doi.org/10.1007/s10571-009-9437-8
---------- CHICAGO ----------
Meyer, M., Gonzalez Deniselle, M.C., Garay, L.I., Monachelli, G.G., Lima, A., Roig, P., et al. "Stage dependent effects of progesterone on motoneurons and glial cells of wobbler mouse spinal cord degeneration" . Cellular and Molecular Neurobiology 30, no. 1 (2010) : 123-135.
http://dx.doi.org/10.1007/s10571-009-9437-8
---------- MLA ----------
Meyer, M., Gonzalez Deniselle, M.C., Garay, L.I., Monachelli, G.G., Lima, A., Roig, P., et al. "Stage dependent effects of progesterone on motoneurons and glial cells of wobbler mouse spinal cord degeneration" . Cellular and Molecular Neurobiology, vol. 30, no. 1, 2010, pp. 123-135.
http://dx.doi.org/10.1007/s10571-009-9437-8
---------- VANCOUVER ----------
Meyer, M., Gonzalez Deniselle, M.C., Garay, L.I., Monachelli, G.G., Lima, A., Roig, P., et al. Stage dependent effects of progesterone on motoneurons and glial cells of wobbler mouse spinal cord degeneration. Cell. Mol. Neurobiol. 2010;30(1):123-135.
http://dx.doi.org/10.1007/s10571-009-9437-8