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Abstract:

1. Synthesis of oxytocin (OT) and arginine-vasopressin (AVP) is increased in induced models of Type I diabetes, such as the streptozotocin model. However, these parameters have not yet been evaluated in spontaneous models, such as the nonobese diabetic mouse (NOD). Therefore, we studied in the magnocellular cells of the paraventricular nucleus (PVN) of nondiabetic and diabetic 16-week-old female NOD mice and control C57B1/6 mice, the immunocytochemistry of OT and AVP peptides and their mRNA expression, using nonisotopic in situ hybridization (ISH). 2. In nondiabetic and diabetic NOD female mice, the number of OT- and AVP-immunoreactive cells were similar to those of the controls, whereas immunoreaction intensity was significantly higher for both peptides in diabetic NOD as compared with nondiabetic NOD and control C57B1/6 mice. 3. ISH analysis showed that the number of OT mRNA-containing cells was in the same range in the three groups, whereas higher number of AVP mRNA expressing cells was found in diabetic NOD mice. However, the intensity of hybridization signal was also higher for both OT and AVP mRNA in the diabetic group as compared with nondiabetic NOD and control mice. 4. Blood chemistry demonstrated that haematrocrit, total plasma proteins, urea, sodium, and potassium were within normal limits in diabetic mice. Thus, NOD mice were neither hypernatremic nor dehydrated. 5. We suggest that upregulation of OT and AVP reflects a high-stress condition in the NOD mice. Diabetes may affect neuropeptide-producing cells of the PVN, with the increased AVP and OT playing a deleterious role on the outcome of the disease.

Registro:

Documento: Artículo
Título:Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse
Autor:Saravia, F.E.; Gonzalez, S.L.; Roig, P.; Alves, V.; Homo-Delarche, F.; De Nicola, A.F.
Filiación:Laboratory of Neuroendocrine Biochemistry, Instituto de Biologia y Medicina Experimental, Buenos Aires, Argentina
Department of Human Biochemistry, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
CNRS UMR 8603, Universite Paris v, Hopital Necker, Paris, France
Instituto Universitario de Ciencias de la Salud, Fundación Barceló, Buenos Aires, Argentina
Laboratory of Neuroendocrine Biochemistry, Instituto de Biologia y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Diabetes mellitus; Nonobese diabetic (NOD) mouse; Oxytocin; Paraventricular nucleus; Vasopressin; argipressin; messenger RNA; neuropeptide; oxytocin; potassium; protein; sodium; urea; animal experiment; animal model; article; blood chemistry; controlled study; experimental diabetes mellitus; female; giant cell; hematocrit; hypernatremia; immunocytochemistry; immunoreactivity; in situ hybridization; mouse; nonhuman; peptide synthesis; priority journal; protein blood level; protein expression; signal transduction; stress; thalamus midline nucleus; Animals; Diabetes Mellitus, Type 1; Female; Immunohistochemistry; In Situ Hybridization; Mice; Mice, Inbred C57BL; Mice, Inbred NOD; Oxytocin; Paraventricular Hypothalamic Nucleus; RNA, Messenger; Specific Pathogen-Free Organisms; Vasopressins
Año:2001
Volumen:21
Número:1
Página de inicio:15
Página de fin:27
DOI: http://dx.doi.org/10.1023/A:1007165127420
Título revista:Cellular and Molecular Neurobiology
Título revista abreviado:Cell. Mol. Neurobiol.
ISSN:02724340
CODEN:CMNED
CAS:Oxytocin, 50-56-6; RNA, Messenger; Vasopressins, 11000-17-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v21_n1_p15_Saravia

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Citas:

---------- APA ----------
Saravia, F.E., Gonzalez, S.L., Roig, P., Alves, V., Homo-Delarche, F. & De Nicola, A.F. (2001) . Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse. Cellular and Molecular Neurobiology, 21(1), 15-27.
http://dx.doi.org/10.1023/A:1007165127420
---------- CHICAGO ----------
Saravia, F.E., Gonzalez, S.L., Roig, P., Alves, V., Homo-Delarche, F., De Nicola, A.F. "Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse" . Cellular and Molecular Neurobiology 21, no. 1 (2001) : 15-27.
http://dx.doi.org/10.1023/A:1007165127420
---------- MLA ----------
Saravia, F.E., Gonzalez, S.L., Roig, P., Alves, V., Homo-Delarche, F., De Nicola, A.F. "Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse" . Cellular and Molecular Neurobiology, vol. 21, no. 1, 2001, pp. 15-27.
http://dx.doi.org/10.1023/A:1007165127420
---------- VANCOUVER ----------
Saravia, F.E., Gonzalez, S.L., Roig, P., Alves, V., Homo-Delarche, F., De Nicola, A.F. Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse. Cell. Mol. Neurobiol. 2001;21(1):15-27.
http://dx.doi.org/10.1023/A:1007165127420