Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

1. Wobbler mice suffer an autosomal recessive mutation producing severe motoneuron degeneration and dense astrogliosis, with increased levels of glial fibrillary acidic protein (GFAP) in the spinal cord and brain stem. They have been considered animal models of amyotrophic lateral sclerosis and infantile spinal muscular atrophy. 2. Using Wobbler mice and normal littermates, we investigated the effects of the membrane-active steroid Lazaroid U-74389F on the number of GFAP-expressing astrocytes and glucocorticoid receptors (GR). Lazaroids are inhibitors of oxygen radical- induced lipid peroxidation, and proved beneficial in cases of CNS injury and ischemia. 3. Four days after pellet implantation of U-74389F into Wobbler mice, hyperplasia and hypertophy of GFAP-expressing astrocytes were apparent in the spinal cord ventral and dorsal horn, areas showing already intense astrogliosis in untreated Wobbler mice. In control mice, U-74389F also produced astrocyte hyperplasia and hypertophy in the dorsal horn and hyperplasia in the ventral-lateral funiculi of the cord. 4. Given in vivo U- 74389F did not change GR in spinal cord of Wobbler or control mice, in line with the concept that it is active in membranes but does not bind to GR. Besides, U-74390F did not compete for [3H]dexamethasone binding when added in vitro. 5. The results suggest that stimulation of proliferation and size of GFAP-expressing astrocytes by U-74389F may be a novel mechanism of action of this compound. The Wobbler mouse may be a valuable animal model for further pharmacological testing of glucocorticoid and nonglucocorticoid steroids in neurodegenerative diseases.

Registro:

Documento: Artículo
Título:The 21-aminosteroid U-74389F increases the number of glial fibrillary acidic protein-expressing astrocytes in the spinal cord of control and Wobbler mice
Autor:Deniselle, M.C.G.; Gonzalez, S.L.; Piroli, G.G.; Lima, A.E.; De Nicola, A.F.
Filiación:Laboratory of Neuroendocrine Biochemistry, Instituto de Biologia Y Medicina Experimental-CONICET, Buenos Aires, Argentina
Departamento de Bioquímica, Facultad de Medicina, UBA, Buenos Aires, Argentina
Laboratory of Neuroendocrine Biochemistry, Instituto de Biologia Y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:21-aminosteroid; astrocytes; glial fibrillary acidic protein; spinal cord; U-74389F; Wobbler mice; 11beta,17beta dihydroxy 17 (1 propynyl)androsta 1,4,6 trien 3 one; 21 aminosteroid; 21 [4 [2,6 bis(1 pyrrolidinyl) 4 pyrimidinyl] 1 piperazinyl]pregna 1,4,9(11) triene 3,20 dione; dexamethasone; glial fibrillary acidic protein; glucocorticoid; glucocorticoid receptor; lazaroid; tritium; animal cell; animal experiment; animal model; animal tissue; article; astrocyte; astrocytosis; cell hyperplasia; cell proliferation; cell size; controlled study; female; hypertrophy; male; mouse; nerve cell degeneration; nonhuman; priority journal; spinal cord; subcutaneous drug administration
Año:1996
Volumen:16
Número:1
Página de inicio:61
Página de fin:72
DOI: http://dx.doi.org/10.1007/BF02578387
Título revista:Cellular and Molecular Neurobiology
Título revista abreviado:CELL. MOL. NEUROBIOL.
ISSN:02724340
CODEN:CMNED
CAS:11beta,17beta dihydroxy 17 (1 propynyl)androsta 1,4,6 trien 3 one, 74915-58-5; 21 [4 [2,6 bis(1 pyrrolidinyl) 4 pyrimidinyl] 1 piperazinyl]pregna 1,4,9(11) triene 3,20 dione, 111668-89-4; dexamethasone, 50-02-2; tritium, 10028-17-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v16_n1_p61_Deniselle

Referencias:

  • Aizenman, Y., De Vellis, J., Synergistic action of thyroid hormone, insulin and hydrocortisone on astrocyte differentiation (1987) Brain Res., 414, pp. 301-308
  • Bracken, M.B., Shepard, M.J., Collins, W.F., A randomized, controlled trial of methylprednisolone or naloxone in the treatment of acture spinal cord injury (1990) N. Engl. J. Med., 322, pp. 1405-1411
  • Braughler, J.M., Hall, E.D., Effects of multi-dose methylprednisolone sodium succinate administration on injured cat spinal cord neurofilament degradation and energy metabolism (1984) J. Neurosurg., 61, pp. 290-295
  • Braughler, J.M., Pregenzer, J.F., Chase, R.L., Duncan, L.A., Jacobsen, E.J., McCall, J.M., Novel 21-amino steroids as potent inhibitors of iron-dependent lipid peroxidation (1987) J. Biol. Chem., 262, pp. 10438-10440
  • Cohen, G., Author, A.P., (1982) Pathology of Oxygen, , Academic Press, New York
  • Coirini, H., Magariños, A.M., De Nicola, A.F., Rainbow, T.C., McEwen, B.S., Further studies of brain aldosterone binding sites employing new mineralocorticoid and glucocorticoid receptor markers in vitro (1985) Brain Res., 361, pp. 212-216
  • De Nicola, A.F., Steroid hormones and neuronal regeneration (1993) Advances in Neurology, 59, pp. 199-206. , F. J. Seil, Ed., Raven Press, New York
  • De Nicola, A.F., Moses, D.F., Gonzalez, S., Orti, E., Adrenocorticoid action in the spinal cord: Some unique molecular properties of glucocorticoid receptors (1989) Cell. Mol. Neurobiol., 9, pp. 179-192
  • Duchen, L.W., Falconer, D.S., Stricht, S.J., Hereditary progressive neurogenic muscular atrophy in the mouse (1966) J. Physiol. (London), 183, pp. 53P-55P
  • Eddleston, M., Mucke, L., Molecular profile of reactive astrocytes-Implications for their role in neurologic disease (1993) Neuroscience, 54, pp. 15-36
  • Eng, L.F., Glial fibrillary acidic protein (GFAP): The major protein of glial intermediate filaments in differentiated astrocytes (1985) J. Neuroimmunol., 8, pp. 203-214
  • Eng, L.F., Ghirnikar, R.S., GFAP and astrogliosis (1994) Brain Pathol., 4, pp. 229-237
  • Ferrini, M., Gonzalez, S., Antakly, T., De Nicola, A.F., Immunocytochemical localization of glucocorticoid receptors in the spinal cord: Effects of adrenalectomy, glucocorticoid treatment and spinal cord transection (1993) Cell. Mol. Neurobiol., 13, pp. 387-398
  • Fleishaker, J.C., Peters, G.R., Cathcart, K.S., Evaluation of the pharmacokinetics and tolerability of tirilazad mesylate, a 21-aminosteroid free radical scavenger. I. Single-dose administration (1993) J. Clin. Pharmacol., 33, pp. 175-181
  • Gage, F.H., Olejniczak, P., Armstrong, D.M., Astrocytes are important for sprouting in the septo-hippocampal circuit (1988) Exp. Neurol., 102, pp. 2-12
  • Garcia-Segura, L.M., Liquin, S., Parducz, Naftolin, F., Gonadal hormone regulation of glial fibrillary acidic protein immunoreactivity and glial ultrastructure in the rat neuroendocrine hypothalamus (1994) Glia, 10, pp. 59-69
  • Giulian, D., Corpuz, M., Microglial secretion products and their impact on the nervous system (1993) Advances in Neurology, 59, pp. 315-320. , (F. J. Seil, Ed.), Raven Press, New York
  • Goss, J.R., Finch, C.E., Morgan, D.G., Age-related changes in glial fibrillary acidic protein mRNA in the mouse brain (1991) Neurobiol. Aging, 12, pp. 165-170
  • Hall, E.D., Beneficial effects of the 21-aminosteroid U-74006F in acute CNS trauma and hypovolemic shock (1987) Acta Anaesth. Belg., 38, pp. 421-425
  • Hall, E.D., Neuroprotective actions of glucocorticoid and nonglucocorticoid steroids in acute neuronal injury (1993) Cell. Mol. Neurobiol., 13, pp. 415-432
  • Krieger, C., Perry, T.L., Hanse, S., Mitsumoto, H., Honore, T., Excitatory amino acid receptor antagonist in murine motoneurone disease (the Wobbler mouse) (1992) Can. J. Neurol. Sci., 19, pp. 462-465
  • Kupersmith, M.J., Kaufman, D., Paty, D.W., Ebers, G., McFarland, H., Johnson, K., Reingold, S., Whitaker, J., Megadose corticosteroids in multiple sclerosis (1994) Neurology, 4, pp. 1-4
  • Laage, S., Zobel, G., Jockusch, H., Astrocyte overgrowth in the brain stem and spinal cord of mice affected by spinal atrophy, Wobbler (1988) Dev. Neurosci., 10, pp. 190-198
  • La Mantia, L., Eoli, M., Milanese, C., Salmaggi, A., Dufour, A., Torri, V., Double-blind trial of dexamethasone versus methylprednisolone in multiple sclerosis acute relapses (1994) Eur. Neurol., 34, pp. 199-203
  • Taping, N.J., Nichols, N.R., Day, J.R., Finch, C.E., Corticosterone differentially regulates the bilateral response of astrocyte mRNAs in the hippocampus to entorhinal cortex lesions in male rats (1991) Mol. Brain Res., 10, pp. 291-297
  • Laping, N.J., Teter, B., Nichols, N.R., Rozovsky, I., Finch, C.E., Glial fibrillary acidic protein: Regulation by hormones, cytokines, and growth factors (1994) Brain Pathol., 1, pp. 259-275
  • Leetsma, J.E., Animal model: Motor neuron disease in the wobbler (wr/wr) mouse (1980) Am. J. Pathol., 100, pp. 811-814
  • McCall, J.M., Hall, E.D., Braughler, J.M., A new class of 21-aminosteroids which are useful for stroke and trauma (1989) Steroids and Diseases of the Central Nervous System, pp. 69-80. , (R. Capildeo, Ed.), John Wiley & Sons, New York
  • McKeon, R.J., Schreiber, R.C., Rudge, J.S., Silver, J., Reduction of neurite outgrowth in a model of glial scarring following CNS injury is correlated with the expression of inhibitory molecules on reactive astrocytes (1991) J. Neurosci., 11, pp. 3398-3411
  • Mitsumoto, H., Bradley, W.G., Murine motor neuron disease (the Wobbler Mouse): Degeneration and regeneration of the lower motor neuron (1982) Brain Res., 105, pp. 811-834
  • Moses, D.F., Gonzalez, S., McEwen, B.S., De Nicola, A.F., Glucocorticoid type II receptors of the spinal cord show lower affinity than hippocampal type II receptors. Binding parameters obtained with different experimental protocols (1991) J. Steroid Biochem. Mol. Biol., 39, pp. 5-12
  • Muller, H.W., Matthiessen, H.P., Schmalenbach, C., Schroeder, W.O., Glial support of CNS neuronal survival, neurite growth and regeneration (1991) Rest. Neurol. Neurosci., 2, pp. 229-232
  • Murayama, S., Inoue, K., Kawakami, H., Bouidin, T.W., Suzuki, K., A unique pattern of astrocytosis in the primary motor area in amyotrophic lateral sclerosis (1991) Acta Neuropathol. (Berlin), 82, pp. 456-461

Citas:

---------- APA ----------
Deniselle, M.C.G., Gonzalez, S.L., Piroli, G.G., Lima, A.E. & De Nicola, A.F. (1996) . The 21-aminosteroid U-74389F increases the number of glial fibrillary acidic protein-expressing astrocytes in the spinal cord of control and Wobbler mice. Cellular and Molecular Neurobiology, 16(1), 61-72.
http://dx.doi.org/10.1007/BF02578387
---------- CHICAGO ----------
Deniselle, M.C.G., Gonzalez, S.L., Piroli, G.G., Lima, A.E., De Nicola, A.F. "The 21-aminosteroid U-74389F increases the number of glial fibrillary acidic protein-expressing astrocytes in the spinal cord of control and Wobbler mice" . Cellular and Molecular Neurobiology 16, no. 1 (1996) : 61-72.
http://dx.doi.org/10.1007/BF02578387
---------- MLA ----------
Deniselle, M.C.G., Gonzalez, S.L., Piroli, G.G., Lima, A.E., De Nicola, A.F. "The 21-aminosteroid U-74389F increases the number of glial fibrillary acidic protein-expressing astrocytes in the spinal cord of control and Wobbler mice" . Cellular and Molecular Neurobiology, vol. 16, no. 1, 1996, pp. 61-72.
http://dx.doi.org/10.1007/BF02578387
---------- VANCOUVER ----------
Deniselle, M.C.G., Gonzalez, S.L., Piroli, G.G., Lima, A.E., De Nicola, A.F. The 21-aminosteroid U-74389F increases the number of glial fibrillary acidic protein-expressing astrocytes in the spinal cord of control and Wobbler mice. CELL. MOL. NEUROBIOL. 1996;16(1):61-72.
http://dx.doi.org/10.1007/BF02578387