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Abstract:

This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated splenocytes when TRH (10-6 M-10-12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin. © 1995 Plenum Publishing Corporation.

Registro:

Documento: Artículo
Título:TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH
Autor:Raiden, S.; Polack, E.; Nahmod, V.; Labeur, M.; Holsboer, F.; Arzt, E.
Filiación:Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Clinical Institute, Max-Planck Institute of Psychiatry, Kraepelinstr. 10, Munich, D-80804, Germany
Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:immuneneuroendocrine interactions; lymphocyte activation; TRH; TRH-receptor; messenger rna; protirelin; protirelin receptor; animal cell; animal experiment; article; controlled study; dna synthesis; female; immunostimulation; intraperitoneal drug administration; lymphocyte activation; nonhuman; priority journal; rat; Animal; Antibodies; Blotting, Northern; Cells, Cultured; DNA; Female; Gene Expression; Human; Leukocytes, Mononuclear; Lymphocyte Activation; Lymphocytes; Protirelin; Rats; Rats, Wistar; Receptors, Thyrotropin-Releasing Hormone; RNA, Messenger; Spleen; Stimulation, Chemical; Support, Non-U.S. Gov't
Año:1995
Volumen:15
Número:5
Página de inicio:242
Página de fin:249
DOI: http://dx.doi.org/10.1007/BF01540881
Título revista:Journal of Clinical Immunology
Título revista abreviado:J Clin Immunol
ISSN:02719142
CODEN:JCIMD
CAS:Antibodies; DNA, 9007-49-2; Protirelin, 24305-27-9; Receptors, Thyrotropin-Releasing Hormone; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02719142_v15_n5_p242_Raiden

Referencias:

  • Besedovsky, H., del Rey, A., Immune-neuroendocrine circuits: Integrative role of cytokines (1992) Frontiers Neuroendocrinol, 13, pp. 61-94
  • Reichlin, S., Neuroendocrine-immune interactions (1993) New Engl J Med, 329, pp. 1246-1253
  • Haddad, E., Mashaly, M., In vivo effects of TRH, T<inf>3</inf> and cGH on antibody production and T- and B-lymphocytes proliferation in immature male chickens (1991) Immunol Investig, 20, pp. 557-568
  • Villa-Verde, D., De Mello-Coelho, V., Farias de Oliveira, D.A., Dardenne, M., Savino, W., Pleitropic influence of triiodo thyronine on thymus physiology (1993) Endocrinol, 133, pp. 867-875
  • Pierpaoli, W., Baroni, C., Fabris, N., Sorkin, E., Hormones and immunological capacity. II: Reconstitution of antibody production in hormonally deficient mice by somatotropic hormone, thyrotropic hormone and thyroxin (1969) Immunol, 16, pp. 217-230
  • Blalock, J.E., Johnson, H.M., Smith, E.M., Torres, B.A., Enhancement of the in vitro antibody response by thyrotropin (1984) Biochem Biophys Res Comm, 125, pp. 30-34
  • Kruger, T.E., Blalock, J.E., Cellular requirements for thyrotropin enhancement of in vitro antibody production (1986) J Immunol, 137, pp. 197-200
  • Harbour, D.V., Leon, S., Keating, C., Hughes, T.K., Thyrotropin modulates B-cell function through specific bioactive receptors (1990) Progr NeuroEndocrinlmmunol, 3, pp. 266-276
  • Komorowski, J., Zylinska, K., Pawlikowski, M., Stepien, H., Stimulatory effect of thyrotropin (TSH) on interleukin-2 (IL-2) release from human peripheral blood lymphocytes. A dose response study in vitro (1993) Horm Metab Res, 25, pp. 598-599
  • Kruger, T.E., Smith, L.R., Harbour, D.V., Blalock, J.E., Thyrotropin: an endogenous regulator of the in vitro immune response (1989) J Immunol, 142, pp. 744-747
  • Harbour, D.V., Kruger, T.E., Coppenhaver, D., Smith, E.M., Meyer, W.J., Differential expression and regulation of thyrotropin (TSH) in T cell lines (1989) Mol Cell Endocrinol, 64, pp. 229-241
  • Harbour DV, Jyoti M, Kang WL: Evidence for in vitro and in vivo regulation of cytokines by thyrotropin releasing hormone (TRH). Abstract from the 22nd Annual Meeting of the Society for Neuroscience, Anaheim, California, 1992; Lersch, C., Hammer, C., Krombach, F., Dancygier, H., Effect of thyrotrophin-releasing hormone (TRH) on the chemiluminescence (cl) activity of human mononuclear cells (1989) J Clin Lab Immunol, 28, pp. 69-71
  • Komorowski, J., Stepien, H., Pawlikowski, M., Increased interleukin-2 levels during standard TRH test in man (1994) Neuropeptides, 27, pp. 151-156
  • Pawlikowski, M., Zerek-Melén, G., Winczyk, K., Thyroliberin (TRH) increases thymus cell proliferation in rats (1992) Neuropeptides, 23, pp. 199-202
  • Harbour, D.V., Smith, E.M., Meyer, W.J., Molt-4 T Lymphocyte production of TSH and expression of TRH receptors: An in vitro neuroendocrine model (1988) FASEB J, 1, p. A 1642
  • Becú, D., Libertun, C., Comparative maturation of the regulation of prolactin and throtropin by serotonin and thyrotropin-releasing hormone in male and female rats (1982) Endocrinol, 110, pp. 1879-1884
  • Sowers, J.R., Tempel, G., Resch, G., Colantino, M., Pituitary response to TRH and LHRH in spontaneously hypertensive rats (1978) Proc Soc Exp Biol Med, 159, pp. 397-399
  • Selenkow, H.A., Pascasio, F.M., Cline, M.J., Immunobiological studies of thyrotropin (1962) Ciba Foundation Colloquia on Endocrinology. Vol XIV: Immunoassays of Hormones, pp. 248-263. , G.E.W., Wolstenholme, M.P.P., Cameron, Little, Brown, Boston
  • Labeur, M., Nahmod, V.E., Finkielman, S., Arzt, E., Lesions of the medial septal nucleus produce a long-lasting inhibition of T lymphocyte proliferation (1991) Neurosci Lett, 125, pp. 129-132
  • Böyun, W., Separation of lymphocytes from peripheral blood and bone marrow (1968) Scand J Clin Invest, 21, pp. 77-89
  • Arzt, E., Stelzer, G., Renner, U., Lange, M., Müller, O.A., Stalla, G.K., Interleukin-2 and interleukin-2 receptor expression in human corticotrophic adenoma and murine pituitary cell cultures (1992) J Clin Invest, 90, pp. 1944-1951
  • Arzt, E., Fernández, Finocchiaro, L., Criscuolo, M., Díaz, A., Finkielman, S., Nahmod, V., Immunomodulation by indoleamines: Serotonin and melatonin action on DNA and interferon-gamma synthesis by human peripheral blood mononuclear cells (1988) J Clin Immunol, 8, pp. 513-520
  • Straub, R.E., Frech, G.C., Joho, R.H., Gershengorn, M.C., Expression cloning of a cDNA encoding the mouse pituitary thyrotrophin-releasing hormone receptor (1990) Proc Natl Acad Sci USA, 87, pp. 9514-9518
  • Arzt, E., Sauer, J., Pollmächer, T., Labeur, M., Holsboer, F., Reul, J., Stalla, G.K., Glucocorticoids suppress interleukin-1 receptor antagonist synthesis following induction by endotoxin (1994) Endocrinol, 134, pp. 672-677
  • Koibuchi, N., Gibbs, R.B., Suzuki, M., Pfaff, D.W., Thyroidectomy induces fos-like immunoreactivity within thyrotrophin-releasing hormone-expressing neurons located in the paraventricular nucleus of the adult rat hypothalamus (1991) Endocrinol, 129, pp. 3208-3216
  • Ladram, A., Bulant, M., Delfour, A., Montagne, J.J., Vaudry, H., Nicolas, P., Modulation of the biological activity of thyrotropin-releasing hormone by alternate processing of pro-TRH (1994) Biochimie, 76, pp. 320-328
  • Pagesy, P., Croissandeau, G., Le Defniet, M., Peillion, F., Li, Y., Detection of thyrotrophin-releasing hormone (TRH) mRNA by the reverse transcription-polymerize chain reaction in the human normal and tumbrel anterior pituitary (1992) Biochem Biophys Res Comm, 182, pp. 182-187
  • Bruhn, T.O., Bolduc, T.G., Maclean, D.B., Jackson, I.M.D., ProTRH peptides are synthesized and secreted by anterior pituitary cells in long-term culture (1991) Endocrinol, 129, pp. 556-558
  • Le Dafniet, M., Lafebre, P., Barret, A., Mechain, C., Feinstein, M.C., Brandi, A.M., Peillon, F., Normal and adenomatous human pituitaries secrete thyrotrophin-releasing hormone in vitro: Modulation by dopamine, haloperidol and somatostatin (1990) J Clin Endocrinol Metab, 71, pp. 480-486
  • Griffiths, E., Thyrotropin-releasing hormone: Endocrine and central effects (1985) Psychoneuroendocrinol, 10, pp. 225-235
  • Fuse, Y., Polk, D.H., Lam, R.Q., Fisher, D.A., Distribution of thyrotrophin-releasing hormone (TRH) and precursor peptide (TRH-Gay) in adult rat tissues (1990) Endocrinol, 127, pp. 2501-2505
  • Jackson, I.M.D., Thyrotrophin-releasing hormone (1982) N Engl J Med, 306, p. 145
  • Manaker, S., Winokur, A., Rhodes, C.H., Rainbow, T.C., Autoradiographic localization of thyrotrophin-releasing hormone (TRH) receptors in human spinal cord (1985) Neurology, 35, p. 328
  • Clevenger, C., Silliman, A., Hanley-Hyde, J., Prystowsky, M., Requirement for prolactin during cell cycle regulated gene expression in cloned T-lymphocytes (1992) Endocrinol, 130, pp. 3216-3222
  • Hartmann, D., Holaday, J., Bernton, E., Inhibition of lymphocyte proliferation by antibodies to prolactin (1989) FASEB J, 3, pp. 2194-2202
  • Sundar, S., Becker, K., Cierpial, M., Carpenter, M., Rankin, L., Fleener, S., Ritchie, J., Weiss, J., Intracerebroventricular infusion of interleukin-1 rapidly decreases peripheral cellular immune responses (1989) Proc Natl Acad Sci USA, 86, pp. 6398-6402
  • Sundar, S., Cierpial, M., Kilts, C., Ritchie, J., Weiss, J., Brain IL-1-induced immunosuppression occurs through activation of both pituitary-adrenal axis and sympathetic nervous system by corticotrophin-releasing factor (1990) J Neurosci, 10, pp. 3701-3706
  • Sapolsky, R., Rivier, C., Yamamoto, G., Plotsky, P., Vale, W., Interleukin-1 stimulates the secretion of hypothalamic corticotrophin-releasing factor (1987) Science, 238, pp. 522-524
  • Suda, T., Tozawa, F., Ushiyama, T., Sumitomo, T., Yamada, M., Demura, H., Interleukin-1 stimulates corticotrophin-releasing factor gene expression in rat hypothalamus (1990) Endocrinol, 126, pp. 1223-1228
  • Kakucska, I., Romero, L., Clark, B.D., Rondeel, J., Qi, Y., Alex, S., Emerson, C., Lechan, R.M., Suppression of thyrotrophin-releasing hormone gene expression by interleukin-1 beta in the rat: Implications for nonthyroidal illness (1994) Neuroendocrinol, 59, pp. 129-137
  • Dubois, J.M., Dayer, J.M., Siegrist-Kaiser, C.A., Burger, A.G., Human recombinant interleukin-1 β decreases plasma thyroid hormone and thyroid stimulating hormone levels in rats (1988) Endocrinology, 123, pp. 2175-2181
  • Irwin, M., Hauger, R., Brown, M., Central corticotrophin-releasing hormone activates the sympathetic nervous system and reduces immune function: Increased responsivity of the aged rat (1992) Endocrinol, 131, pp. 1047-1053
  • Webster, E., Tracey, D., Jutila, M., Wolfe, S., Souza, E., Corticotrophin-releasing factor receptors in mouse spleen: Identification of receptor-bearing cells as resident macrophages (1990) Endocrinol, 127, pp. 440-452
  • Singh, V., Fudenberg, H.H., Binding of [125I]-corticotrophin-releasing factor to blood immunocytes and its reduction in Alzheimer's disease (1988) Immunol Lett, 18, pp. 5-8
  • Lux, W.E., Feuerstein, G., Faden, A.J., Thyrotrophin-releasing hormone reverses experimental anaphylactic shock through non-endorphin-related mechanisms (1983) Eur J Pharmacol, 90, pp. 301-302
  • Faden, A., Hill, T., Kubek, M., Changes in TRH immunoreactivity in spinal cord after experimental spinal injury (1986) Neuropeptides, 7, pp. 11-18
  • Kiviniemi, H., Laitinen, S., Stahlberg, M., Larmi, M., Kairaluoma, M., Thyrotrophin-releasing hormone in acute pancreatitis. A preliminary report (1986) Acta Chir Scand, 152, pp. 43-47
  • Pierpaoli, W., Chanxian, Y., The involvement of pineal gland and melatonin in immunity and aging. I: Thymus-mediated immunoreconstituting and antiviral activity of thyrotrophin-releasing hormone (1990) J Neuroimmunol, 27, pp. 99-109

Citas:

---------- APA ----------
Raiden, S., Polack, E., Nahmod, V., Labeur, M., Holsboer, F. & Arzt, E. (1995) . TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH. Journal of Clinical Immunology, 15(5), 242-249.
http://dx.doi.org/10.1007/BF01540881
---------- CHICAGO ----------
Raiden, S., Polack, E., Nahmod, V., Labeur, M., Holsboer, F., Arzt, E. "TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH" . Journal of Clinical Immunology 15, no. 5 (1995) : 242-249.
http://dx.doi.org/10.1007/BF01540881
---------- MLA ----------
Raiden, S., Polack, E., Nahmod, V., Labeur, M., Holsboer, F., Arzt, E. "TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH" . Journal of Clinical Immunology, vol. 15, no. 5, 1995, pp. 242-249.
http://dx.doi.org/10.1007/BF01540881
---------- VANCOUVER ----------
Raiden, S., Polack, E., Nahmod, V., Labeur, M., Holsboer, F., Arzt, E. TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH. J Clin Immunol. 1995;15(5):242-249.
http://dx.doi.org/10.1007/BF01540881