Artículo

TORTORELLA, H.; JOSEFINA, M.; DE CEREZO, S.; MAZZINI, M.N.; CEREZO, A.S. "The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma" (1983) American Journal of Reproductive Immunology. 4(2):76-82
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

ABSTRACT: Immunodiffusion, immunoelectrophoresis, SDS‐polyacrylamide gel electrophoresis, polyacrylamide gel electrophoresis, ultracentrifugation analysis, and gel permeation chromatography in chaotropic and detergent mediums showed that the human seminal plasma obtained and stored in the usual way is mainly composed by heterodispersed glycoproteins of low molecular weight. The glycoprotein components of the human seminal plasma which do not interact specifically with concanavalin A (con A) were separated by column chromatography on Con A‐Sepharose according to nonspecificion exchange and hydrophobic interactions with the protein. The carbohydrate composition of the glycoproteins and the existence of the N‐acetylgalactosaminylserine (or threonine) linkage, as well as their aggregation properties show that they are mucin‐type glycoproteins. They resulted “immunologically pure’ although the carbohydrate chains show the structural heterodispersion usually found in mucins. The glycoproteins have a common structural pattern for the terminal nonreducing, determinant‐bearing sequences oftheir oligosaccharide chains which, together with the low molecular weights and the known fact that mucus secretions of normal cells contain only one or two types of glycoproteins, suggest that they are fragments produced by endogenous proteolytic degradation of a native mucin. 1983 Munksgaard

Registro:

Documento: Artículo
Título:The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma
Autor:TORTORELLA, H.; JOSEFINA, M.; DE CEREZO, S.; MAZZINI, M.N.; CEREZO, A.S.
Filiación:Centro de Investigaciones En Reproductión
Facultad de Medicina, Departamento de Química Orgánica, Spain
Facultad de Ciencias Exactas Y Naturales
Universidad de Buenos Aires, Argentina
Universidade Federal Do Rio Grande Do Sul, Porto Alegre, Brazil
Departamento de Bioquímica, Argentina
Palabras clave:glycoprotein; mucin; gel chromatography; human; human cell; immunodiffusion; immunoelectrophoresis; male genital system; polyacrylamide gel electrophoresis; sperm; Chemistry; Comparative Study; Glycoproteins; Human; Male; Molecular Weight; Mucins; Semen; Support, Non-U.S. Gov't
Año:1983
Volumen:4
Número:2
Página de inicio:76
Página de fin:82
DOI: http://dx.doi.org/10.1111/j.1600-0897.1983.tb00257.x
Título revista:American Journal of Reproductive Immunology
Título revista abreviado:Am. J. Reprod. Immunol.
ISSN:02717352
CAS:Glycoproteins; Mucins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02717352_v4_n2_p76_TORTORELLA

Referencias:

  • Mazzini, MN, de Cerezo, JMS, Cerezo, AS, Affinity chromatography on Con A‐Sepharose of antigenic fractions of human seminal plasma (1979) J Chromatog., 173, pp. 365-371
  • Shulman, S, Li, TS, Immunochemical studies on human seminal plasma III: Ultracentrifugal studies on human seminal plasma and a purified fraction (1974) Int J Fertil., 19, pp. 19-22
  • Herrmann, WP, Herrman, G, Purification of the specific seminal fluid 3.721S protein (1969) Fertil Steril., 20, pp. 521-525
  • Uhlenbruck, G, Steinhausen, G, Herrmann, WP, Demonstration of organ‐characteristic glycoprotein in human semen (1977) Andrology., 9, pp. 171-178
  • de Cerezo, JMS, Bueno, MP, Skowroñsky, B, Cerezo, AS, Immunoserological, immunohistochemical and immunobiological studies on an antigenic glycoprotein isolated from human seminal plasma (1982) J Reprod Immunol., 4, pp. 11-21
  • Rosengren, J, Pahlman, S, Glad, M, Hjerten, S, Hydrophobic interaction chromatography on non‐charged Sepharose derivatives: Binding of a model protein related to ionic strength, hydrophobicity of the substituent and degree of substitution (1975) Biochem Biophys Acta., 412, pp. 51-61
  • Zaneveld, LJD, Polakoski, KL, Schumacher, GBF, The proteolytic enzyme systems of mammalian genital tract secretion and spermatozoa (1975) Proteases and Biological Control, 2, pp. 683-706. , Reich E, Refkin DB, Shaws E,. Cold Spring Harbor Conferences in Cell Proliferation
  • Morawiecki, A, Dissociation of M‐ and N‐group mucoprotein into subunits in detergent solution (1964) Biochem Biophys Acta., 83, pp. 339-347
  • Hafez, ESE, Transport and survival of spermatozoa in the human female reproductive tract (1978) Recent Progress in Andrology., 203, pp. 203-213
  • Tortorella, H, Mazzini, MN, de Cerezo, JMS, Cerezo, AS, Hydrophobic and ion‐exchange interactions in an “affinity” chromatography of human seminal plasma on Con A‐Sepharose (1981) Anales Asoc Quím Argentina., 69, pp. 171-176
  • Funk, JH, Townstiack, BA, A practical method for detecting ABO agglutinins and agglutinogens in dried bloodstains (1965) J Forensic Sciences., 10, pp. 455-465
  • Reinhold, VN, Gas liquid chromatographic analysis of constituent carbohydrates in glycoproteins (1972) Methods in Enzymology, 25, pp. 244-249. , Ginsbing V,. New York:, Academic Press
  • Mazzini, MN, Cerezo, AS, The carbohydrate and protein composition of the endosperm, embryo and testa of the seed of Gleditsia triacanthos (1979) Journal of the Science of Food and Agriculture, 30, pp. 881-891
  • Halhone, MN, Kleinberg, I, Differential determination of glucose and fructose and glucose‐ and fructose‐yielding substances with anthrone (1972) Anal Biochem., 50, pp. 337-343
  • Johnson, AR, Improved method of hexosamine determination (1971) Anal Biochem., 40, pp. 628-635
  • Dische, Z, Shettles, LB, A specific colour reactive of methyl pentoses and a spectrophotometric micromethod for their determination (1948) J Biochem Comm., 175, pp. 595-603
  • Warren, L, The thiobarbituric acid assay of sialic acid (1959) J Biol Chem., 234, pp. 1971-1975
  • Kochetkov, NK, Chizhov, OS, Kadentsev, VI, Smirnova, GP, Zhukova, IG, Mass spectra of acetylated derivative of sialic acid (1973) Carbohydr Res., 27, pp. 5-10
  • Segrest, JP, Jackson, RLM, Molecular weight determinations in glycoproteins by polyacrylamide gel electrophoresis in SDS (1978) Methods in Enzymology, 28, pp. 98-103. , Ginsbing V,. New York:, Academic Press
  • Fairbanks, G, Steck, TL, Wallach, DFH, Electrophoretic analysis of the major glycopeptides of the human erythrocyte membrane (1971) Biochemistry., 10, pp. 2606-2617
  • Plantner, JJ, Carlson, DM, Studies on mucin‐type glycoproteins; Olefinic amino acids, products of the β‐elimination (1975) Anal Biochem., 65, pp. 153-163
  • Brown, HC, Fletcher, RS, Chemical effects of steric strain (1949) J Am Chem Soc., 71, pp. 1845-1854
  • Reddy, JM, Stark, RA, Zaneveld, LJD, A high molecular weight antifertility factor from human seminal plasma (1979) J Reprod Fertil., 57, pp. 437-446
  • Li, TS, Beling, C, Isolation and characterization of two specific antigens of human seminal plasma (1973) Fertil Steril., 24, pp. 134-144
  • Lippiello, LA, El‐Rubaye, F, Weil, AJ, Human spermatozoa‐coating antigen: Studies on purification (1968) Fertil Steril., 19, pp. 991-998
  • Mann, T, Rottenberg, DA, The carbohydrate of human semen (1966) Journal of Endocrinology, 34, pp. 257-264
  • Lloyd, KO, Glycoproteins with blood group activity (1976) Carbohydrates. International Review of Science. Organic Chemistry. Series II, 7, pp. 251-258. , Aspinall GO,. London:, Butterworths
  • Hermann, WP, Uhlenbruck, G, Carbohydrate moieties of human seminal plasma arylamidases (1976) Andrology., 8, pp. 308-312
  • Hermann, WP, Uhlenbruck, G, Studies on glycoproteins of the human seminal plasma (1972) Z Klin Chem Klin Biochem., 10, pp. 363-366
  • Uhlenbruck, G, Hermann, WP, Steinhauser, G, Blood group substances A prepared from human seminal plasma (1973) Arch Derm Forsch., 246, pp. 47-54
  • Kubicek, R, Lindner, E, Santavy, F, Acides amines dans le plasma seminal de divers mamiferes (1959) Bull Soc Chim Biol., 41, pp. 1345-1359
  • Doehr, SA, Cervical mucus glycoproteins (1977) The glycoconjugates, 1, pp. 239-257. , Horowitz MI, Pigman E,. New York:, Academic Press
  • Pigman, W, Mucus glycoproteins (1977) The glycoconjugates, 1, pp. 131-135. , Horowitz MI, Pigman E,. New York:, Academic Press
  • Harrison, R, Lunt, GG, (1975) Biological Membranes, p. 128. , London:, Blackie and Sons, Ltd
  • Kent, E, Uhlenbruck, G, Uber neuraminsaure haltige: Mucoide ans Menschenerythrocytenstroma ein Bertrag zur Chemie der Agglutinogene (1960) Hoppe-Seylu's Z Physiol Chem., 319, pp. 151-160
  • Lhermitte, M, Lamblin, G, Laffite, JJ, Degand, P, Roussel, P, Mazzuca, M, Human, bronchial‐mucus glycoproteins: A comparison between chemical properties and affinities for lectins (1981) Carbohyd Res., 92, pp. 333-343
  • Singer, SJ, The molecular organization of membranes (1974) Annual Review of Biochemistry, 43, pp. 805-833. , Snell ER,. Palo Alto:, Annual Reviews, Inc
  • Roberts, TK, Boetther, B, Identification of human spermatozoa antigen (1969) J Reprod Fertil., 18, pp. 347-350
  • Menezo, Y, Flechon, JE, Utilization d'une microtechnique pour la determination des activités enzymatiques des spermatozoides et du plasma seminal chez le lapin et le taureau (1973) C R Acad Sc Paris., 277, pp. 1037-1040
  • Williams‐Ashman, HG, Introductory overview of the participation of proteinases and their regulators in mammalian reproductive physiology (1975) Proteases and Biological Control, 2, pp. 677-681. , Reich E, Refkin DB, Shaw E,. Cold Spring Harbor Conferences on Cell Proliferation
  • Tanber, PF, Zaneveld, LJD, Coagulation and liquefaction of human semen (1976) Human Semen and Fertility Regulation in Men, pp. 153-166. , Hafez ESE,. St. Louis:, The C.V. Mosby Company

Citas:

---------- APA ----------
TORTORELLA, H., JOSEFINA, M., DE CEREZO, S., MAZZINI, M.N. & CEREZO, A.S. (1983) . The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma. American Journal of Reproductive Immunology, 4(2), 76-82.
http://dx.doi.org/10.1111/j.1600-0897.1983.tb00257.x
---------- CHICAGO ----------
TORTORELLA, H., JOSEFINA, M., DE CEREZO, S., MAZZINI, M.N., CEREZO, A.S. "The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma" . American Journal of Reproductive Immunology 4, no. 2 (1983) : 76-82.
http://dx.doi.org/10.1111/j.1600-0897.1983.tb00257.x
---------- MLA ----------
TORTORELLA, H., JOSEFINA, M., DE CEREZO, S., MAZZINI, M.N., CEREZO, A.S. "The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma" . American Journal of Reproductive Immunology, vol. 4, no. 2, 1983, pp. 76-82.
http://dx.doi.org/10.1111/j.1600-0897.1983.tb00257.x
---------- VANCOUVER ----------
TORTORELLA, H., JOSEFINA, M., DE CEREZO, S., MAZZINI, M.N., CEREZO, A.S. The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma. Am. J. Reprod. Immunol. 1983;4(2):76-82.
http://dx.doi.org/10.1111/j.1600-0897.1983.tb00257.x