Artículo
Abstract:
ABSTRACT: The antigens isolated from the trichloroacetic acid soluble fraction of human seminal plasma show a polymodal molecular weight heterodispersion. The molecular weights corresponding to the modes of the distribution have limiting values of 2,500‐57,000. Previous results obtained by polyacrylamide gel and cellogel electrophoresis, immunodiffusion and Immunoelectrophoresis, and ultracentrifugation analysis, which suggested homogeneity, were explained by the formation of molecular aggregates, which would also explain why the smaller fragments were not lost during the dialysis carried out in the preparation and purification of the samples. The average molecular weight of the antigens, determined by gel permeation chromatography and using the equation of Svedberg, is in the range of 10,000–40,000. It is suggested that the polymodal heterodispersion together with the associating‐dissociating properties of these products is used to control their immunological behavior. 1981 Munksgaard
Registro:
| Documento: | Artículo |
| Título: | The Molecular Weights and Molecular Weight Heterodispersion of Some Antigens From the Trichloroacetic Acid Soluble Fraction of Human Seminal Plasma |
| Autor: | DE CEREZO, J.M.S.; CEREZO, A.S. |
| Filiación: | Centro de Investigaciones En Reproduction, Facultad de Medicina, Buenos Aires, Argentina Departamento de Química Orgánica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, Buenos Aires, Argentina |
| Palabras clave: | antigens; human seminal plasma; Molecular weight heterodispersion; trichloroacetic soluble fraction; trichloroacetic acid; male genital system; normal human; sperm |
| Año: | 1981 |
| Volumen: | 1 |
| Número: | 5 |
| Página de inicio: | 266 |
| Página de fin: | 268 |
| DOI: | http://dx.doi.org/10.1111/j.1600-0897.1981.tb00052.x |
| Título revista: | American Journal of Reproductive Immunology |
| Título revista abreviado: | Am. J. Reprod. Immunol. |
| ISSN: | 02717352 |
| CAS: | trichloroacetic acid, 14357-05-2, 76-03-9 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02717352_v1_n5_p266_DECEREZO |
Referencias:
- Cerezo, Isolation and characterization of two antigens from human seminal plasma (1974) Int J Fertil, 19, pp. 211-216
- Cerezo, Cerezo, AS, Antigens from human seminal plasma. Part III. Studies on two new antigens from the trichloroacetic acid soluble fraction (1977) Int J Fertil, 22, pp. 162-167
- Gibbons, RA, Physico‐chemical methods for the determination of the purity, molecular size and shape of glycoproteins (1972) Glycoproteins, Part A, pp. 31-128. , Gottschalk A,. Amsterdam, New York:, Elsevier
- Andrews, P, Estimation of molecular size and molecular weights of biological compounds by gel filtration (1970) Methods in Biochemical Analysis, Vol 18, pp. 1-53. , Glick D,. New York:, Interscience
- Svedberg, T, (1940) The Ultracentrifuge, , Oxford:, Charendon
- Dubois, M, Gilles, K, Hamilton, JK, Rebois, PA, Smith, F, A colorimetric method for the determination of sugars (1951) Nature, 168, p. 167
- Steward, MW, (1974) Immunochemistry, p. 18. , London:, Chapman & Hall
- Hepman, A, Rümke, P, Antigens of semen and auto‐immunity against spermatozoa in infertile men (1974) Proc Serono Symposia, Vol 5, pp. 249-269. , Mancini RE, Martini L,. London:, Academic
Citas:
---------- APA ----------
DE CEREZO, J.M.S. & CEREZO, A.S. (1981) . The Molecular Weights and Molecular Weight Heterodispersion of Some Antigens From the Trichloroacetic Acid Soluble Fraction of Human Seminal Plasma. American Journal of Reproductive Immunology, 1(5), 266-268.http://dx.doi.org/10.1111/j.1600-0897.1981.tb00052.x
---------- CHICAGO ----------
DE CEREZO, J.M.S., CEREZO, A.S. "The Molecular Weights and Molecular Weight Heterodispersion of Some Antigens From the Trichloroacetic Acid Soluble Fraction of Human Seminal Plasma" . American Journal of Reproductive Immunology 1, no. 5 (1981) : 266-268.http://dx.doi.org/10.1111/j.1600-0897.1981.tb00052.x
---------- MLA ----------
DE CEREZO, J.M.S., CEREZO, A.S. "The Molecular Weights and Molecular Weight Heterodispersion of Some Antigens From the Trichloroacetic Acid Soluble Fraction of Human Seminal Plasma" . American Journal of Reproductive Immunology, vol. 1, no. 5, 1981, pp. 266-268.http://dx.doi.org/10.1111/j.1600-0897.1981.tb00052.x
---------- VANCOUVER ----------
DE CEREZO, J.M.S., CEREZO, A.S. The Molecular Weights and Molecular Weight Heterodispersion of Some Antigens From the Trichloroacetic Acid Soluble Fraction of Human Seminal Plasma. Am. J. Reprod. Immunol. 1981;1(5):266-268.http://dx.doi.org/10.1111/j.1600-0897.1981.tb00052.x
