Artículo
Rocha-Viegas, L.; Villa, R.; Gutierrez, A.; Iriondo, O.; Shiekhattar, R.; Croce, L.D. "Role of UTX in retinoic acid receptor-mediated gene regulation in leukemia" (2014) Molecular and Cellular Biology. 34(19):3765-3775
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Abstract:
Human UTX, a member of the Jumonji C family of proteins, associates with mixed-lineage leukemia 3/4 complexes. Stimulation with retinoic acid leads to the recruitment of UTX-containing complexes to HOX genes, which results in demethylation of histone H3 lysine 27 and concomitant methylation of histone H3 lysine 4. Here, we show that UTX interacts with the retinoic acid receptor α (RARα) and that this interaction is essential for proper differentiation of leukemic U937 cells in response to retinoic acid. UTX occupies the promoters of several RAR target genes and regulates their transcriptional output by modulating ASH2L complex recruitment. Overexpression of UTX in promyelocytic NB4 cells results in enhanced cellular differentiation upon retinoic acid treatment. Our results show that UTX is important for RAR-mediated transcription and provide insight into the critical role of cross talk between histone H3 lysine 4 methylation and histone H3 lysine 27 demethylation during cellular differentiation. © 2014, American Society for Microbiology.
Registro:
| Documento: | Artículo |
| Título: | Role of UTX in retinoic acid receptor-mediated gene regulation in leukemia |
| Autor: | Rocha-Viegas, L.; Villa, R.; Gutierrez, A.; Iriondo, O.; Shiekhattar, R.; Croce, L.D. |
| Filiación: | Center for Genomic Regulation, Barcelona, Spain Universitat Pompeu Fabra, Barcelona, Spain Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, United States Institucio´ Catalana de Recerca i Estudis Avanc¸ats, Barcelona, Spain Departamento de Qui´mica Biolo´gica, FCEN, IFIBYNE-CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina Adolf Butenamdt Institut, Molecular Biology, LMU, Munich, Germany Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA, United States |
| Palabras clave: | histone H3; lysine; protein; retinoic acid; retinoic acid receptor alpha; unclassified drug; utx protein; antineoplastic agent; ASH2L protein, human; DNA binding protein; histone; histone demethylase; nuclear protein; retinoic acid; retinoic acid receptor; retinoic acid receptor alpha; transcription factor; UTX protein, human; Article; cell differentiation; cell maturation; chromatin immunoprecipitation; controlled study; flow cytometry; fluorescence activated cell sorting; gene activation; gene control; gene expression; gene overexpression; gene silencing; gene targeting; genetic transcription; genetic transfection; human; human cell; immunoprecipitation; in vivo study; promoter region; promyelocytic leukemia; protein depletion; protein interaction; quantitative analysis; retrovirus infection; reverse transcription polymerase chain reaction; transcription initiation; U937 cell line; article; drug effect; gene expression regulation; genetics; HEK293 cell line; leukemia; metabolism; pathology; tumor cell line; Antineoplastic Agents; Cell Differentiation; Cell Line, Tumor; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; HEK293 Cells; Histone Demethylases; Histones; Humans; Leukemia; Nuclear Proteins; Promoter Regions, Genetic; Receptors, Retinoic Acid; Transcription Factors; Tretinoin; U937 Cells |
| Año: | 2014 |
| Volumen: | 34 |
| Número: | 19 |
| Página de inicio: | 3765 |
| Página de fin: | 3775 |
| DOI: | http://dx.doi.org/10.1128/MCB.00839-14 |
| Título revista: | Molecular and Cellular Biology |
| Título revista abreviado: | Mol. Cell. Biol. |
| ISSN: | 02707306 |
| CODEN: | MCEBD |
| CAS: | lysine, 56-87-1, 6899-06-5, 70-54-2; protein, 67254-75-5; retinoic acid, 302-79-4; histone, 9062-68-4 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02707306_v34_n19_p3765_RochaViegas |
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Citas:
---------- APA ----------
Rocha-Viegas, L., Villa, R., Gutierrez, A., Iriondo, O., Shiekhattar, R. & Croce, L.D. (2014) . Role of UTX in retinoic acid receptor-mediated gene regulation in leukemia. Molecular and Cellular Biology, 34(19), 3765-3775.http://dx.doi.org/10.1128/MCB.00839-14
---------- CHICAGO ----------
Rocha-Viegas, L., Villa, R., Gutierrez, A., Iriondo, O., Shiekhattar, R., Croce, L.D. "Role of UTX in retinoic acid receptor-mediated gene regulation in leukemia" . Molecular and Cellular Biology 34, no. 19 (2014) : 3765-3775.http://dx.doi.org/10.1128/MCB.00839-14
---------- MLA ----------
Rocha-Viegas, L., Villa, R., Gutierrez, A., Iriondo, O., Shiekhattar, R., Croce, L.D. "Role of UTX in retinoic acid receptor-mediated gene regulation in leukemia" . Molecular and Cellular Biology, vol. 34, no. 19, 2014, pp. 3765-3775.http://dx.doi.org/10.1128/MCB.00839-14
---------- VANCOUVER ----------
Rocha-Viegas, L., Villa, R., Gutierrez, A., Iriondo, O., Shiekhattar, R., Croce, L.D. Role of UTX in retinoic acid receptor-mediated gene regulation in leukemia. Mol. Cell. Biol. 2014;34(19):3765-3775.http://dx.doi.org/10.1128/MCB.00839-14
