Artículo

Druker, J.; Liberman, A.C.; Antunica-Noguerol, M.; Gerez, J.; Paez-Pereda, M.; Rein, T.; Iñiguez-Lluhí, J.A.; Holsboer, F.; Arzta, E. "RSUME enhances glucocorticoid receptor SUMOylation and transcriptional activity" (2013) Molecular and Cellular Biology. 33(11):2116-2127
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Abstract:

Glucocorticoid receptor (GR) activity is modulated by posttranslational modifications, including phosphorylation, ubiquitination, and SUMOylation. The GR has three SUMOylation sites: lysine 297 (K297) and K313 in the N-terminal domain (NTD) and K721 within the ligand-binding domain. SUMOylation of the NTD sites mediates the negative effect of the synergy control motifs of GR on promoters with closely spaced GR binding sites. There is scarce evidence on the role of SUMO conjugation to K721 and its impact on GR transcriptional activity. We have previously shown that RSUME (RWD-containing SUMOylation enhancer) increases protein SUMOylation.We nowdemonstrate that RSUME interacts with the GR and increases its SUMOylation. RSUME regulatesGRtranscriptional activity and the expression of its endogenous target genes, FKBP51 and S100P. RSUME uncovers a positive role for the third SUMOylation site, K721, on GR-mediated transcription, demonstrating thatGRSUMOylation acts positively in the presence of a SUMOylation enhancer. Both mutation of K721 and small interfering RNA-mediated RSUME knockdown diminish GRIP1 coactivator activity. RSUME, whose expression is induced under stress conditions, is a key factor in heat shock-inducedGRSUMOylation. These results show that inhibitory and stimulatorySUMOsites are present in theGRand at higher SUMOylation levels the stimulatory one becomes dominant. © 2013, American Society for Microbiology.

Registro:

Documento: Artículo
Título:RSUME enhances glucocorticoid receptor SUMOylation and transcriptional activity
Autor:Druker, J.; Liberman, A.C.; Antunica-Noguerol, M.; Gerez, J.; Paez-Pereda, M.; Rein, T.; Iñiguez-Lluhí, J.A.; Holsboer, F.; Arzta, E.
Filiación:Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), CONICET, Partner Institute of the Max Planck Society, and DFBMC, FCEN, Universidad de Buenos Aires, Buenos Aires, Argentina
Max Planck Institute of Psychiatry, Munich, Germany
Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI, United States
Palabras clave:glucocorticoid receptor; nuclear receptor coactivator 2; small interfering RNA; article; controlled study; female; gene activity; gene expression; gene mutation; gene silencing; genetic transcription; human; human cell; in vitro study; priority journal; protein interaction; protein processing; sumoylation; Animals; Arginine; Carrier Proteins; Cell Line; Cercopithecus aethiops; COS Cells; Heat-Shock Response; Mutation; Nerve Tissue Proteins; Protein Structure, Tertiary; Rats; Receptors, Glucocorticoid; Sumoylation; Transcription Factors; Transcriptional Activation
Año:2013
Volumen:33
Número:11
Página de inicio:2116
Página de fin:2127
DOI: http://dx.doi.org/10.1128/MCB.01470-12
Título revista:Molecular and Cellular Biology
Título revista abreviado:Mol. Cell. Biol.
ISSN:02707306
CODEN:MCEBD
CAS:Arginine, 74-79-3; Carrier Proteins; GRIP1 protein, human; Nerve Tissue Proteins; RSUME protein, human; Receptors, Glucocorticoid; Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02707306_v33_n11_p2116_Druker

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Citas:

---------- APA ----------
Druker, J., Liberman, A.C., Antunica-Noguerol, M., Gerez, J., Paez-Pereda, M., Rein, T., Iñiguez-Lluhí, J.A.,..., Arzta, E. (2013) . RSUME enhances glucocorticoid receptor SUMOylation and transcriptional activity. Molecular and Cellular Biology, 33(11), 2116-2127.
http://dx.doi.org/10.1128/MCB.01470-12
---------- CHICAGO ----------
Druker, J., Liberman, A.C., Antunica-Noguerol, M., Gerez, J., Paez-Pereda, M., Rein, T., et al. "RSUME enhances glucocorticoid receptor SUMOylation and transcriptional activity" . Molecular and Cellular Biology 33, no. 11 (2013) : 2116-2127.
http://dx.doi.org/10.1128/MCB.01470-12
---------- MLA ----------
Druker, J., Liberman, A.C., Antunica-Noguerol, M., Gerez, J., Paez-Pereda, M., Rein, T., et al. "RSUME enhances glucocorticoid receptor SUMOylation and transcriptional activity" . Molecular and Cellular Biology, vol. 33, no. 11, 2013, pp. 2116-2127.
http://dx.doi.org/10.1128/MCB.01470-12
---------- VANCOUVER ----------
Druker, J., Liberman, A.C., Antunica-Noguerol, M., Gerez, J., Paez-Pereda, M., Rein, T., et al. RSUME enhances glucocorticoid receptor SUMOylation and transcriptional activity. Mol. Cell. Biol. 2013;33(11):2116-2127.
http://dx.doi.org/10.1128/MCB.01470-12