Artículo

Echeverría, P.C.; Mazaira, G.; Erlejman, A.; Gomez-Sanchez, C.; Pilipuk, G.P.; Galigniana, M.D. "Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin β" (2009) Molecular and Cellular Biology. 29(17):4788-4797
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Abstract:

Glucocorticoid receptor (GR) is cytoplasmic in the absence of ligand and localizes to the nucleus after steroid binding. Previous evidence demonstrated that the hsp90-based heterocomplex bound to GR is required for the efficient retrotransport of the receptor to the nuclear compartment. We examined the putative association of GR and its associated chaperone heterocomplex with structures of the nuclear pore. We found that importin β and the integral nuclear pore glycoprotein Nup62 interact with hsp90, hsp70, p23, and the TPR domain proteins FKBP52 and PP5. Nup62 and GR were able to interact in a more efficient manner when chaperoned by the hsp90-based heterocomplex. Interestingly, the binding of hsp70 and p23 to Nup62 does not require the presence of hsp90, whereas the association of FKBP52 and PP5 is hsp90 dependent, as indicated by the results of experiments where the hsp90 function was disrupted with radicicol. The ability of both FKBP52 and PP5 to interact with Nup62 was abrogated in cells overexpressing the TPR peptide. Importantly, GR cross-linked to the hsp90 heterocomplex was able to translocate to the nucleus in digitonin-permeabilized cells treated with steroid, suggesting that GR could pass through the pore in its untransformed state. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Registro:

Documento: Artículo
Título:Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin β
Autor:Echeverría, P.C.; Mazaira, G.; Erlejman, A.; Gomez-Sanchez, C.; Pilipuk, G.P.; Galigniana, M.D.
Filiación:Fundación Instituto Leloir-IIBBA/CONICET, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Argentina
Division of Endocrinology, G. V. (Sonny) Montgomery VA Medical Center, University of Mississippi Medical Center, Jackson, MS, United States
Av. Patricias Argentinas 435, Buenos Aires (C1405BWE), Argentina
Palabras clave:digitonin; glucocorticoid receptor; glycoprotein; glycoprotein nup62; heat shock protein 70; heat shock protein 90; karyopherin beta; protein Fkbp52; protein p23; protein pp5; radicicol; steroid; tetratricopeptide repeat protein; unclassified drug; animal cell; article; cell membrane permeability; controlled study; human; human cell; mouse; nonhuman; nuclear import; nuclear pore; nuclear pore complex; priority journal; protein binding; protein cross linking; protein expression; protein function; protein protein interaction; structure analysis; tetratricopeptide repeat; Active Transport, Cell Nucleus; Animals; beta Karyopherins; Cell Line; Glycoproteins; HSP70 Heat-Shock Proteins; HSP90 Heat-Shock Proteins; Humans; Intramolecular Oxidoreductases; Mice; Multiprotein Complexes; Nuclear Pore; Nuclear Pore Complex Proteins; Receptors, Glucocorticoid; Tacrolimus Binding Proteins
Año:2009
Volumen:29
Número:17
Página de inicio:4788
Página de fin:4797
DOI: http://dx.doi.org/10.1128/MCB.00649-09
Handle:http://hdl.handle.net/20.500.12110/paper_02707306_v29_n17_p4788_Echeverria
Título revista:Molecular and Cellular Biology
Título revista abreviado:Mol. Cell. Biol.
ISSN:02707306
CODEN:MCEBD
CAS:digitonin, 11024-24-1; radicicol, 12772-57-5; Glycoproteins; HSP70 Heat-Shock Proteins; HSP90 Heat-Shock Proteins; Intramolecular Oxidoreductases, 5.3.-; Multiprotein Complexes; NUP62 protein, mouse; Nuclear Pore Complex Proteins; Receptors, Glucocorticoid; Tacrolimus Binding Proteins, 5.2.1.-; beta Karyopherins; prostaglandin-E synthase, 5.3.99.3; tacrolimus binding protein 4, 5.2.1.-; tissue-factor-pathway inhibitor 2
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_02707306_v29_n17_p4788_Echeverria.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02707306_v29_n17_p4788_Echeverria

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Citas:

---------- APA ----------
Echeverría, P.C., Mazaira, G., Erlejman, A., Gomez-Sanchez, C., Pilipuk, G.P. & Galigniana, M.D. (2009) . Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin β. Molecular and Cellular Biology, 29(17), 4788-4797.
http://dx.doi.org/10.1128/MCB.00649-09
---------- CHICAGO ----------
Echeverría, P.C., Mazaira, G., Erlejman, A., Gomez-Sanchez, C., Pilipuk, G.P., Galigniana, M.D. "Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin β" . Molecular and Cellular Biology 29, no. 17 (2009) : 4788-4797.
http://dx.doi.org/10.1128/MCB.00649-09
---------- MLA ----------
Echeverría, P.C., Mazaira, G., Erlejman, A., Gomez-Sanchez, C., Pilipuk, G.P., Galigniana, M.D. "Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin β" . Molecular and Cellular Biology, vol. 29, no. 17, 2009, pp. 4788-4797.
http://dx.doi.org/10.1128/MCB.00649-09
---------- VANCOUVER ----------
Echeverría, P.C., Mazaira, G., Erlejman, A., Gomez-Sanchez, C., Pilipuk, G.P., Galigniana, M.D. Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin β. Mol. Cell. Biol. 2009;29(17):4788-4797.
http://dx.doi.org/10.1128/MCB.00649-09