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Wedemeyer, C.; Martín, J.Z.S.; Ballestero, J.; Gómez-Casati, M.E.; Torbidoni, A.V.; Fuchs, P.A.; Bettler, B.; Elgoyhen, A.B.; Katz, E. "Activation of presynaptic GABA b(1a,2) receptors inhibits synaptic transmission at mammalian inhibitory cholinergic olivocochlear-hair cell synapses" (2013) Journal of Neuroscience. 33(39):15477-15487
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Abstract:

The synapse between olivocochlear (OC) neurons and cochlear mechanosensory hair cells is cholinergic, fast, and inhibitory. The inhibitory sign of this cholinergic synapse is accounted for by the activation of Ca 2+ -permeable postsynaptic α9α10 nicotinic receptors coupled to the opening of hyperpolarizing Ca 2+ -activated small-conductance type 2 (SK2)K + channels. Acetylcholine (ACh) release at this synapse is supported by both P/Q- and N-type voltage-gated calcium channels (VGCCs). Although the OC synapse is cholinergic, an abundantOCGABAinnervation is present along themammaliancochlea. The role of this neurotransmitter at theOCefferent innervation, however, is for the most part unknown.Weshow thatGABAfails to evoke fast postsynaptic inhibitory currents in apical developing inner and outer hair cells. However, electrical stimulation ofOCefferent fibers activates presynapticGABA B(1a,2) receptors [GABAB (1a,2) Rs] that downregulate the amount of ACh released at the OC-hair cell synapse, by inhibiting P/Q-type VGCCs. We confirmed the expression of GABA B Rs at OC terminals contacting the hair cells by coimmunostaining for GFP and synaptophysin in transgenic mice expressing GABA B1 -GFP fusion proteins. Moreover, coimmunostaining with antibodies against the GABA synthetic enzyme glutamic acid decarboxylase and synaptophysin support the idea that GABA is directly synthesized at OC terminals contacting the hair cells during development. Thus, we demonstrate for the first time a physiological role for GABA in cochlear synaptic function. In addition, our data suggest that the GABA B1a isoform selectively inhibits release at efferent cholinergic synapses. © 2013 the authors.

Registro:

Documento: Artículo
Título:Activation of presynaptic GABA b(1a,2) receptors inhibits synaptic transmission at mammalian inhibitory cholinergic olivocochlear-hair cell synapses
Autor:Wedemeyer, C.; Martín, J.Z.S.; Ballestero, J.; Gómez-Casati, M.E.; Torbidoni, A.V.; Fuchs, P.A.; Bettler, B.; Elgoyhen, A.B.; Katz, E.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular Dr. Hector N. Torres, 1428 Buenos Aires, Argentina
Tercera Cátedra de Farmacología, 1121 Buenos Aires, Argentina
Johns Hopkins University, School of Medicine, Baltimore, MD 21205, United States
Department of Biomedicine, Institute of Physiology, University of Basel, CH-4056 Basel, Switzerland
Departamento de Fisiología, Biología Molecular y Celular, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:4 aminobutyric acid B (1a,2) receptor; 4 aminobutyric acid B receptor; baclofen; N type voltage gated calcium channel; neurotransmitter; P type voltage gated calcium channel; Q type voltage gated calcium channel; synaptophysin; unclassified drug; voltage gated calcium channel; acetylcholine release; animal tissue; article; cholinergic nerve cell; cochlea; controlled study; efferent nerve; electrophysiology; electrostimulation; hair cell; hearing; immunohistochemistry; inhibitory postsynaptic potential; mouse; nerve fiber; nonhuman; olivocochlea; priority journal; synapse; synaptic transmission; Acetylcholine; Animals; Calcium Channels, P-Type; Calcium Channels, Q-Type; Cholinergic Neurons; Electric Stimulation; gamma-Aminobutyric Acid; Hair Cells, Auditory; Inhibitory Postsynaptic Potentials; Mice; Mice, Inbred BALB C; Neurons, Efferent; Receptors, GABA-B; Synapses; Synaptophysin
Año:2013
Volumen:33
Número:39
Página de inicio:15477
Página de fin:15487
DOI: http://dx.doi.org/10.1523/JNEUROSCI.2554-13.2013
Título revista:Journal of Neuroscience
Título revista abreviado:J. Neurosci.
ISSN:02706474
CODEN:JNRSD
CAS:baclofen, 1134-47-0; Acetylcholine, N9YNS0M02X; Calcium Channels, P-Type; Calcium Channels, Q-Type; Receptors, GABA-B; Synaptophysin; gamma-Aminobutyric Acid, 56-12-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v33_n39_p15477_Wedemeyer

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Citas:

---------- APA ----------
Wedemeyer, C., Martín, J.Z.S., Ballestero, J., Gómez-Casati, M.E., Torbidoni, A.V., Fuchs, P.A., Bettler, B.,..., Katz, E. (2013) . Activation of presynaptic GABA b(1a,2) receptors inhibits synaptic transmission at mammalian inhibitory cholinergic olivocochlear-hair cell synapses. Journal of Neuroscience, 33(39), 15477-15487.
http://dx.doi.org/10.1523/JNEUROSCI.2554-13.2013
---------- CHICAGO ----------
Wedemeyer, C., Martín, J.Z.S., Ballestero, J., Gómez-Casati, M.E., Torbidoni, A.V., Fuchs, P.A., et al. "Activation of presynaptic GABA b(1a,2) receptors inhibits synaptic transmission at mammalian inhibitory cholinergic olivocochlear-hair cell synapses" . Journal of Neuroscience 33, no. 39 (2013) : 15477-15487.
http://dx.doi.org/10.1523/JNEUROSCI.2554-13.2013
---------- MLA ----------
Wedemeyer, C., Martín, J.Z.S., Ballestero, J., Gómez-Casati, M.E., Torbidoni, A.V., Fuchs, P.A., et al. "Activation of presynaptic GABA b(1a,2) receptors inhibits synaptic transmission at mammalian inhibitory cholinergic olivocochlear-hair cell synapses" . Journal of Neuroscience, vol. 33, no. 39, 2013, pp. 15477-15487.
http://dx.doi.org/10.1523/JNEUROSCI.2554-13.2013
---------- VANCOUVER ----------
Wedemeyer, C., Martín, J.Z.S., Ballestero, J., Gómez-Casati, M.E., Torbidoni, A.V., Fuchs, P.A., et al. Activation of presynaptic GABA b(1a,2) receptors inhibits synaptic transmission at mammalian inhibitory cholinergic olivocochlear-hair cell synapses. J. Neurosci. 2013;33(39):15477-15487.
http://dx.doi.org/10.1523/JNEUROSCI.2554-13.2013