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Abstract:

Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4-{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N'[4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.41±3.98% reduction; A-843277, 17.52±6.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl- conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly, the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating. © 2013 the authors.

Registro:

Documento: Artículo
Título:The 5-HT5A receptor regulates excitability in the auditory startle circuit: Functional implications for sensorimotor gating
Autor:Curtin, P.C.P.; Medan, V.; Neumeister, H.; Bronson, D.R.; Preuss, T.
Filiación:Graduate Center, City University of New York, New York, NY 10065, United States
Research Foundation, City University of New York, New York, NY10065, United States
Hunter College, City University of New York, New York, NY 10065, United States
Physiology and Molecular Biology Department, School of Science, University of Buenos Aires and IFIBYNE-CONICET, Ciudad Universitaria, Intendente Güiraldes 2160, 1428 Buenos Aires, Argentina
Palabras clave:serotonin 5A receptor; serotonin antagonist; biphenyl derivative; guanidine derivative; N (2,6 dimethoxybenzyl) N' (4 (4 fluorophenyl)thiazol 2 yl)guanidine; N-(2,6-dimethoxybenzyl)-N'-(4-(4-fluorophenyl)thiazol-2-yl)guanidine; potassium chloride; SB 699551 A; SB-699551-A; serotonin 5 receptor; serotonin antagonist; serotonin receptor; thiazole derivative; animal experiment; article; auditory stimulation; behavior; brain electrophysiology; chloride conductance; controlled study; evoked auditory response; excitatory postsynaptic potential; goldfish; Mauthner cell; nerve cell excitability; nerve cell membrane steady potential; nerve cell plasticity; nonhuman; prepulse inhibition; priority journal; sensory gating; startle reflex; swimming; acoustics; animal; cytology; dose response; drug effect; electrostimulation; evoked auditory response; female; inhibitory postsynaptic potential; male; metabolism; methodology; nerve cell; nerve cell inhibition; nerve cell network; nerve tract; patch clamp; physiology; sensory gating; spinal cord; time; Acoustic Stimulation; Acoustics; Animals; Biphenyl Compounds; Dose-Response Relationship, Drug; Electric Stimulation; Evoked Potentials, Auditory; Excitatory Postsynaptic Potentials; Female; Goldfish; Guanidines; Inhibitory Postsynaptic Potentials; Male; Nerve Net; Neural Inhibition; Neural Pathways; Neurons; Patch-Clamp Techniques; Potassium Chloride; Receptors, Serotonin; Sensory Gating; Serotonin Antagonists; Spinal Cord; Startle Reaction; Thiazoles; Time Factors
Año:2013
Volumen:33
Número:24
Página de inicio:10011
Página de fin:10020
DOI: http://dx.doi.org/10.1523/JNEUROSCI.4733-12.2013
Título revista:Journal of Neuroscience
Título revista abreviado:J. Neurosci.
ISSN:02706474
CODEN:JNRSD
CAS:potassium chloride, 7447-40-7; Biphenyl Compounds; Guanidines; N-(2,6-dimethoxybenzyl)-N'-(4-(4-fluorophenyl)thiazol-2-yl)guanidine; Potassium Chloride, 7447-40-7; Receptors, Serotonin; SB-699551-A; Serotonin Antagonists; Thiazoles; serotonin 5 receptor
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v33_n24_p10011_Curtin

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Citas:

---------- APA ----------
Curtin, P.C.P., Medan, V., Neumeister, H., Bronson, D.R. & Preuss, T. (2013) . The 5-HT5A receptor regulates excitability in the auditory startle circuit: Functional implications for sensorimotor gating. Journal of Neuroscience, 33(24), 10011-10020.
http://dx.doi.org/10.1523/JNEUROSCI.4733-12.2013
---------- CHICAGO ----------
Curtin, P.C.P., Medan, V., Neumeister, H., Bronson, D.R., Preuss, T. "The 5-HT5A receptor regulates excitability in the auditory startle circuit: Functional implications for sensorimotor gating" . Journal of Neuroscience 33, no. 24 (2013) : 10011-10020.
http://dx.doi.org/10.1523/JNEUROSCI.4733-12.2013
---------- MLA ----------
Curtin, P.C.P., Medan, V., Neumeister, H., Bronson, D.R., Preuss, T. "The 5-HT5A receptor regulates excitability in the auditory startle circuit: Functional implications for sensorimotor gating" . Journal of Neuroscience, vol. 33, no. 24, 2013, pp. 10011-10020.
http://dx.doi.org/10.1523/JNEUROSCI.4733-12.2013
---------- VANCOUVER ----------
Curtin, P.C.P., Medan, V., Neumeister, H., Bronson, D.R., Preuss, T. The 5-HT5A receptor regulates excitability in the auditory startle circuit: Functional implications for sensorimotor gating. J. Neurosci. 2013;33(24):10011-10020.
http://dx.doi.org/10.1523/JNEUROSCI.4733-12.2013