Artículo

Overstreet, L.S.; Hentges, S.T.; Bumaschny, V.F.; De Souza, F.S.J.; Smart, J.L.; Santangelo, A.M.; Low, M.J.; Westbrook, G.L.; Rubinstein, M. "A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus" (2004) Journal of Neuroscience. 24(13):3251-3259
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Abstract:

Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.

Registro:

Documento: Artículo
Título:A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
Autor:Overstreet, L.S.; Hentges, S.T.; Bumaschny, V.F.; De Souza, F.S.J.; Smart, J.L.; Santangelo, A.M.; Low, M.J.; Westbrook, G.L.; Rubinstein, M.
Filiación:Vollum Institute, Oregon Health and Science University, Portland, OR 97239, United States
Dept. of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR 97239, United States
Ctr. Stud. Weight Reg./Assoc. D., Oregon Health and Science University, Portland, OR 97239, United States
Inst. Invest. Ing. Genet./Biol. M., Depto. de Fisiol., Biol. Molec./Cel., Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Centro de Estudios Cientificos, Casilla 1469, Valdivia, Chile
Inst. Invest. Ing. Genet./Biol. M., Consejo Nac. de Invest. Cie. y Tec., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:BrdU; Dentate granule cell; Green fluorescent protein; Neurogenesis; Neuronal progenitor; Proopiomelanocortin; PSA-nCAM; Transgenic mice; broxuridine; green fluorescent protein; proopiomelanocortin; action potential; animal tissue; article; dentate gyrus; gene; gene expression; granule cell; immunohistochemistry; mouse; newborn; nonhuman; priority journal; stem cell; Action Potentials; Aging; Animals; Biological Markers; Bromodeoxyuridine; Cell Count; Cell Division; Cell Movement; Dentate Gyrus; Exertion; Genes, Reporter; Green Fluorescent Proteins; Luminescent Proteins; Mice; Mice, Inbred C57BL; Mice, Transgenic; Neural Cell Adhesion Molecule L1; Neurons; Pro-Opiomelanocortin; Promoter Regions (Genetics); Sialic Acids; Transgenes
Año:2004
Volumen:24
Número:13
Página de inicio:3251
Página de fin:3259
DOI: http://dx.doi.org/10.1523/JNEUROSCI.5173-03.2004
Título revista:Journal of Neuroscience
Título revista abreviado:J. Neurosci.
ISSN:02706474
CODEN:JNRSD
CAS:broxuridine, 59-14-3; proopiomelanocortin, 66796-54-1; Biological Markers; Bromodeoxyuridine, 59-14-3; Green Fluorescent Proteins, 147336-22-9; Luminescent Proteins; Neural Cell Adhesion Molecule L1; polysialyl neural cell adhesion molecule; Pro-Opiomelanocortin, 66796-54-1; Sialic Acids
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v24_n13_p3251_Overstreet

Referencias:

  • Angevine Jr., J.B., Time of neuron origin in the hippocampal region: An autoradiographic study in the mouse (1965) Exp Neurol [Suppl], 2, pp. 1-70
  • Belluzzi, O., Benedusi, M., Ackman, J., LoTurco, J.J., Electrophysiological differentiation of new neurons in the olfactory bulb (2003) J Neurosci, 23, pp. 10411-10418
  • Biebl, M., Cooper, C.M., Winkler, J., Kuhn, H.G., Analysis of neurogenesis and programmed cell death reveals a self-renewing capacity in the adult rat brain (2000) Neurosci Lett, 291, pp. 17-20
  • Cameron, H.A., McKay, R.D., Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus (2001) J Comp Neurol, 435, pp. 406-417
  • Carleton, A., Petreanu, L.T., Lansford, R., Alvarez-Buylla, A., Lledo, P.M., Becoming a new neuron in the adult olfactory bulb (2003) Nat Neurosci, 6, pp. 507-518
  • Cowley, M.A., Smart, J.L., Rubinstein, M., Cerdan, M.G., Diano, S., Horvath, T.L., Cone, R.D., Low, M.J., Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus (2001) Nature, 411, pp. 480-484
  • Dayer, A.G., Ford, A.A., Cleaver, K.M., Yassaee, M., Cameron, H.A., Short-term and long-term survival of new neurons in the rat dentate gyrus (2003) J Comp Neurol, 460, pp. 563-572
  • Filippov, V., Kronenberg, G., Pivneva, T., Reuter, K., Steiner, B., Wang, L.P., Yamaguchi, M., Kempermann, G., Subpopulation of nestin-expressing progenitor cells in the adult murine hippocampus shows electrophysiological and morphological characteristics of astrocytes (2003) Mol Cell Neurosci, 23, pp. 373-382
  • Fukuda, S., Kato, F., Tozuka, Y., Yamaguchi, M., Miyamoto, Y., Hisatsune, T., Two distinct subpopulations of nestin-positive cells in adult mouse dentate gyrus (2003) J Neurosci, 23, pp. 9357-9366
  • Gerrero, M.R., McEvilly, R.J., Turner, E., Lin, C.R., O'Connell, S., Jenne, K.J., Hobbs, M.V., Rosenfeld, M.G., Brn-3.0: A POU-domain protein expressed in the sensory, immune, and endocrine systems that functions on elements distinct from known octamer motifs (1993) Proc Natl Acad Sci USA, 90, pp. 10841-10845
  • Gong, S., Zheng, C., Doughty, M.L., Losos, K., Didkovsky, N., Schambra, U.B., Nowak, N.J., Heintz, N., A gene expression atlas of the central nervous system based on bacterial artificial chromosomes (2003) Nature, 425, pp. 917-925
  • Hastings, N.B., Gould, E., Rapid extension of axons into the CA3 region by adult-generated granule cells (1999) J Comp Neurol, 413, pp. 146-154
  • Hayes, N.L., Nowakowski, R.S., Dynamics of cell proliferation in the adult dentate gyrus of two inbred strains of mice (2002) Brain Res Dev Brain Res, 134, pp. 77-85
  • Huguenard, J.R., Hamill, O.P., Prince, D.A., Developmental changes in Na+ conductances in rat neocortical neurons: Appearance of a slowly inactivating component (1988) J Neurophysiol, 59, pp. 778-795
  • Jones, S.P., Rahimi, O., O'Boyle, M.P., Diaz, D.L., Claiborne, B.J., Maturation of granule cell dendrites after mossy fiber arrival in hippocampal field CA3 (2003) Hippocampus, 13, pp. 413-427
  • Kaplan, M.S., Hinds, J.W., Neurogenesis in the adult rat: Electron microscopic analysis of light radioautographs (1977) Science, 197, pp. 1092-1094
  • Kuhn, H.G., Dickinson-Anson, H., Gage, F.H., Neurogenesis in the dentate gyrus of the adult rat: Age-related decrease of neuronal progenitor proliferation (1996) J Neurosci, 16, pp. 2027-2033
  • Liu, B., Hammer, G.D., Rubinstein, M., Mortrud, M., Low, M.J., Identification of DNA elements cooperatively activating proopiomelanocortin gene expression in the pituitary glands of transgenic mice (1992) Mol Cell Biol, 12, pp. 3978-3990
  • Liu, J., Solway, K., Messing, R.O., Sharp, F.R., Increased neurogenesis in the dentate gyrus after transient global ischemia in gerbils (1998) J Neurosci, 18, pp. 7768-7778
  • Liu, S., Wang, J., Zbu, D., Fu, Y., Lukowiak, K., Lu, Y., Generation of functional inhibitory neurons in the adult rat hippocampus (2003) J Neurosci, 23, pp. 732-736
  • Liu, X., Tilwallis, S., Ye, G., Lio, P.A., Pasternak, J.F., Trommer, B.L., Morphologic and electrophysiologic maturation in developing dentate granule cells (2000) Brain Res, 856, pp. 202-212
  • Liu, Y.B., Lio, P.A., Pasternak, J.F., Trommer, B.L., Developmental changes in membrane properties and postsynaptic currents of granule cells in rat dentate gyrus (1996) J Neurophysiol, 76, pp. 1074-1088
  • Lois, C., Alvarez-Buylla, A., Long-distance neuronal migration in the adult mammalian brain (1994) Science, 264, pp. 1145-1148
  • Magavi, S.S., Leavitt, B.R., Macklis, J.D., Induction of neurogenesis in the neocortex of adult mice (2000) Nature, 405, pp. 951-955
  • McEwen, B.S., Stress and hippocampal plasticity (1999) Annu Rev Neurosci, 22, pp. 105-122
  • Miyata, T., Maeda, T., Lee, J.E., NeuroD is required for differentiation of the granule cells in the cerebellum and hippocampus (1999) Genes Dev, 13, pp. 1647-1652
  • Nottebohm, F., Neuronal replacement in adult brain (2002) Brain Res Bull, 57, pp. 737-749
  • Palmer, T.D., Willhoite, A.R., Gage, F.H., Vascular niche for adult hippocampal neurogenesis (2000) J Comp Neurol, 425, pp. 479-494
  • Picken Bahrey, H.L., Moody, W.J., Early development of voltage-gated ion currents and firing properties in neurons of the mouse cerebral cortex (2003) J Neurophysiol, 89, pp. 1761-1773
  • Poulin, G., Lebel, M., Chamberland, M., Paradis, F.W., Drouin, J., Specific protein-protein interaction between basic helix-loop-helix transcription factors and homeoproteins of the Pitx family (2000) Mol Cell Biol, 20, pp. 4826-4837
  • Rochefort, C., Gheusi, G., Vincent, J.D., Lledo, P.M., Enriched odor exposure increases the number of newborn neurons in the adult olfactory bulb and improves odor memory (2002) J Neurosci, 22, pp. 2679-2689
  • Rubinstein, M., Mortrud, M., Liu, B., Low, M.J., Rat and mouse proopiomelanocortin gene sequences target tissue-specific expression to the pituitary gland but not to the hypothalamus of transgenic mice (1993) Neuroendocrinology, 58, pp. 373-380
  • Santarelli, L., Saxe, M., Gross, C., Surget, A., Battaglia, F., Dulawa, S., Weisstaub, N., Hen, R., Requirement of hippocampal neurogenesis for the behavioral effects of antidepressants (2003) Science, 301, pp. 805-809
  • Schlessinger, A.R., Cowan, W.M., Gottlieb, D.J., An autoradiographic study of the time of origin and the pattern of granule cell migration in the dentate gyrus of the rat (1975) Comp Neurol, 159, pp. 149-175
  • Schwab, M.H., Bartholomae, A., Heimrich, B., Feldmeyer, D., Druffel-Augustin, S., Goebbels, S., Naya, F.J., Nave, K.A., Neuronal basic helix-loop-helix proteins (NEX and BETA2/Neuro D) regulate terminal granule cell differentiation in the hippocampus (2000) J Neurosci, 20, pp. 3714-3724
  • Seki, T., Expression patterns of immature neuronal markers PSA-NCAM, CRMP-4 and NeuroD in the hippocampus of young adult and aged rodents (2002) J Neurosci Res, 70, pp. 327-334
  • Seki, T., Arai, Y., Highly polysialylated neural cell adhesion molecule (NCAM-H) is expressed by newly generated granule cells in the dentate gyrus of the adult rat (1993) J Neurosci, 13, pp. 2351-2358
  • Seki, T., Arai, Y., Temporal and spatial relationships between PSA-NCAM-expressing, newly generated granule cells, and radial glia-like cells in the adult dentate gyrus (1999) J Comp Neurol, 410, pp. 503-513
  • Smith, M.D., Dawson, S.J., Latchman, D.S., The Brn-3a transcription factor induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins (1997) Mol Cell Biol, 17, pp. 345-354
  • Snyder, J.S., Kee, N., Wojtowicz, J.M., Effects of adult neurogenesis on synaptic plasticity in the rat dentate gyrus (2001) J Neurophysiol, 85, pp. 2423-2431
  • Song, H., Stevens, C.F., Gage, F.H., Astroglia induce neurogenesis from adult neural stem cells (2002) Nature, 417, pp. 39-44
  • Song, H.J., Stevens, C.F., Gage, F.H., Neural stem cells from adult hippocampus develop essential properties of functional CNS neurons (2002) Nat Neurosci, 5, pp. 392-394
  • Tyzio, R., Represa, A., Jorquera, I., Ben-Ari, Y., Gozlan, H., Aniksztejn, L., The establishment of GABAergic and glutamatergic synapses on CA1 pyramidal neurons is sequential and correlates with the development of the apical dendrite (1999) J Neurosci, 19, pp. 10372-10382
  • Van Praag, H., Christie, B.R., Sejnowski, T.J., Gage, F.H., Running enhances neurogenesis, learning, and long-term potentiation in mice (1999) Proc Natl Acad Sci USA, 96, pp. 13427-13431
  • Van Praag, H., Kempermann, G., Gage, F.H., Running increases cell proliferation and neurogenesis in the adult mouse dentate gyrus (1999) Nat Neurosci, 2, pp. 266-270
  • Van Praag, H., Schinder, A.F., Christie, B.R., Toni, N., Palmer, T.D., Gage, F.H., Functional neurogenesis in the adult hippocampus (2002) Nature, 415, pp. 1030-1034
  • Wang, S., Scott, B.W., Wojtowicz, J.M., Heterogenous properties of dentate granule neurons in the adult rat (2000) J Neurobiol, 42, pp. 248-257
  • Winner, B., Cooper-Kuhn, C.M., Aigner, R., Winkler, J., Kuhn, H.G., Long-term survival and cell death of newly generated neurons in the adult rat olfactory bulb (2002) Eur J Neurosci, 16, pp. 1681-1689
  • Yamaguchi, M., Saito, H., Suzuki, M., Mori, K., Visualization of neurogenesis in the central nervous system using nestin promoter-GFP transgenic mice (2000) NeuroReport, 11, pp. 1991-1996
  • Young, J.I., Otero, V., Cerdan, M.G., Falzone, T.L., Chan, E.C., Low, M.J., Rubinstein, M., Cell-specific and developmentally regulated expression of proopiomelanocortin genomic fragments in hypothalamic and hindbrain neurons of transgenic mice (1998) J Neurosci, 18, pp. 6631-6640
  • Zhang, L.I., Poo, M.M., Electrical activity and development of neural circuits (2001) Nat Neurosci [Suppl], 4, pp. 1207-1214

Citas:

---------- APA ----------
Overstreet, L.S., Hentges, S.T., Bumaschny, V.F., De Souza, F.S.J., Smart, J.L., Santangelo, A.M., Low, M.J.,..., Rubinstein, M. (2004) . A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus. Journal of Neuroscience, 24(13), 3251-3259.
http://dx.doi.org/10.1523/JNEUROSCI.5173-03.2004
---------- CHICAGO ----------
Overstreet, L.S., Hentges, S.T., Bumaschny, V.F., De Souza, F.S.J., Smart, J.L., Santangelo, A.M., et al. "A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus" . Journal of Neuroscience 24, no. 13 (2004) : 3251-3259.
http://dx.doi.org/10.1523/JNEUROSCI.5173-03.2004
---------- MLA ----------
Overstreet, L.S., Hentges, S.T., Bumaschny, V.F., De Souza, F.S.J., Smart, J.L., Santangelo, A.M., et al. "A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus" . Journal of Neuroscience, vol. 24, no. 13, 2004, pp. 3251-3259.
http://dx.doi.org/10.1523/JNEUROSCI.5173-03.2004
---------- VANCOUVER ----------
Overstreet, L.S., Hentges, S.T., Bumaschny, V.F., De Souza, F.S.J., Smart, J.L., Santangelo, A.M., et al. A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus. J. Neurosci. 2004;24(13):3251-3259.
http://dx.doi.org/10.1523/JNEUROSCI.5173-03.2004