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Abstract:

Multiple types of high-voltage-activated Ca2+ channels trigger neurotransmitter release at the mammalian central synapse. Among them, the ω-conotoxin GVIA-sensitive N-type channels and the ω-Aga-IVA-sensitive P/Q- type channels mediate fast synaptic transmission. However, at most central synapses, it is not known whether the contributions of different Ca2+ channel types to synaptic transmission remain stable throughout postnatal development. We have addressed this question by testing type-specific Ca2+ channel blockers at developing central synapses. Our results indicate that N- type channels contribute to thalamic and cerebellar IPSCs only transiently during early postnatal period and P/Q-type channels predominantly mediate mature synaptic transmission, as we reported previously at the brainstem auditory synapse formed by the calyx of Held. In fact, Ca2+ currents directly recorded from the auditory calyceal presynaptic terminal were identified as N-, P/Q-, and R-types at postnatal day 7 (P7) to P10 but became predominantly P/Q-type at P13. In contrast to thalamic and cerebellar IPSCs and brainstem auditory EPSCs, N-type Ca2+ channels persistently contribute to cerebral cortical EPSCs and spinal IPSCs throughout postnatal months. Thus, in adult animals, synaptic transmission is predominantly mediated by P/Q-type channels at a subset of synapses and mediated synergistically by multiple types of Ca2+ channels at other synapses.

Registro:

Documento: Artículo
Título:Developmental changes in calcium channel types mediating central synaptic transmission
Autor:Iwasaki, S.; Momiyama, A.; Uchitel, O.D.; Takahashi, T.
Filiación:Department of Neurophysiology, Univ. of Tokyo Faculty of Medicine, Tokyo 113-0033, Japan
Laboratory of Cerebral Structure, Natl. Inst. for Physiological Sci., Myodaiji, Okazaki 444-8585, Japan
Departamento de Ciencias Biologicas, Lab. de Fisiol. y Biol., Molecular, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Palabras clave:Central synapse; N-type calcium channels; P/Q-type calcium channels; Postnatal development; Slice; Transmitter release; calcium channel; neurotransmitter; omega conotoxin; animal tissue; article; brain cortex; calcium current; controlled study; GABAergic system; nerve ending; neurotransmitter release; nonhuman; postnatal development; priority journal; rat; spinal cord dorsal horn; synapse; synaptic transmission; 2-Amino-5-phosphonovalerate; 6-Cyano-7-nitroquinoxaline-2,3-dione; Age Factors; Animals; Brain Chemistry; Calcium Channel Blockers; Calcium Channels, N-Type; Calcium Channels, R-Type; Central Nervous System; Evoked Potentials; Excitatory Amino Acid Antagonists; Excitatory Postsynaptic Potentials; gamma-Aminobutyric Acid; Glycine Agents; omega-Agatoxin IVA; omega-Conotoxins; Organ Culture Techniques; Rats; Rats, Wistar; Strychnine; Synaptic Transmission
Año:2000
Volumen:20
Número:1
Página de inicio:59
Página de fin:65
Título revista:Journal of Neuroscience
Título revista abreviado:J. Neurosci.
ISSN:02706474
CODEN:JNRSD
CAS:2-Amino-5-phosphonovalerate, 76726-92-6; 6-Cyano-7-nitroquinoxaline-2,3-dione, 115066-14-3; Calcium Channel Blockers; Calcium Channels, N-Type; Calcium Channels, R-Type; Excitatory Amino Acid Antagonists; gamma-Aminobutyric Acid, 56-12-2; Glycine Agents; omega-Agatoxin IVA; omega-Conotoxins; Strychnine, 57-24-9; voltage-dependent calcium channel (P-Q type)
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v20_n1_p59_Iwasaki

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Citas:

---------- APA ----------
Iwasaki, S., Momiyama, A., Uchitel, O.D. & Takahashi, T. (2000) . Developmental changes in calcium channel types mediating central synaptic transmission. Journal of Neuroscience, 20(1), 59-65.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v20_n1_p59_Iwasaki [ ]
---------- CHICAGO ----------
Iwasaki, S., Momiyama, A., Uchitel, O.D., Takahashi, T. "Developmental changes in calcium channel types mediating central synaptic transmission" . Journal of Neuroscience 20, no. 1 (2000) : 59-65.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v20_n1_p59_Iwasaki [ ]
---------- MLA ----------
Iwasaki, S., Momiyama, A., Uchitel, O.D., Takahashi, T. "Developmental changes in calcium channel types mediating central synaptic transmission" . Journal of Neuroscience, vol. 20, no. 1, 2000, pp. 59-65.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v20_n1_p59_Iwasaki [ ]
---------- VANCOUVER ----------
Iwasaki, S., Momiyama, A., Uchitel, O.D., Takahashi, T. Developmental changes in calcium channel types mediating central synaptic transmission. J. Neurosci. 2000;20(1):59-65.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v20_n1_p59_Iwasaki [ ]