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Abstract:

Voltage-dependent Ca2+ channels are a major pathway for Ca2+ entry in neurons. We have studied the electrophysiological, pharmacological, and molecular properties of voltage-gated Ca2+ channels in motoneurons of the rat facial nucleus in slices of the brainstem. Most facial motoneurons express both low voltage-activated (LVA) and high voltage-activated (HVA) Ca2+ channel currents. The HVA current is composed of a number of pharmacologically separable components, including 30% of N-type and ~5% of L-type. Despite the dominating role of P-type Ca2+ channels in transmitter release at facial motoneuron terminals described in previous studies, these channels were not present in the cell body. Remarkably, most of the HVA current was carried through a new type of Ca2+ channel that is resistant to toxin and dihydropyridine block but distinct from the R-type currents described in other neurons. Using reverse transcription followed by PCR amplification (RT-PCR) with a powerful set of primers designed to amplify all HVA subtypes of the α1-subunit, we identified a highly heterogeneous expression pattern of Ca2+ channel α1-subunit mRNA in individual neurons consistent with the Ca2+ current components found in the cell bodies and axon terminals. We detected mRNA for α(1A) in 86% of neurons, α(1B) in 59%, α(1C) in 18%, (α(1D) in 18%, and α(1E) in 59%. Either α(1A) or α(1B) mRNAs (or both) were present in all neurons, together with various other α1-subunit mRNAs. The most frequently occurring combination was α(1A) with α(1B) and α(1E). Taken together, these results demonstrate that the Ca2+ channel pattern found in facial motoneurons is highly distinct from that found in other brainstem motoneurons.

Registro:

Documento: Artículo
Título:Single-cell RT-PCR and functional characterization of Ca2+ channels in motoneurons of the rat facial nucleus
Autor:Plant, T.D.; Schirra, C.; Katz, E.; Uchitel, O.D.; Konnerth, A.
Filiación:I. Physiologisches Institut, Universität des Saarlandes, 66421 Homburg, Germany
Institut für Pharmakologie, Freie Universität Berlin, 14195 Berlin, Germany
Departamento de Biología, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina
Departamento de Ciencas Biologicas, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina
Palabras clave:α1-subunit; ω- conotoxin; ω-agatoxin-GVIA; ω-agatoxin-IVA; ω-conotoxin-MVIIC; Calcium channel; Calcium current; Facial nucleus; Motoneuron; Single-cell RT- PCR; calcium; calcium channel; dihydropyridine; omega conotoxin; alpha chain; animal cell; animal experiment; animal tissue; article; channel gating; facial nerve nucleus; gene amplification; membrane conductance; membrane electrophysiology; motoneuron; muscle reinnervation; nerve ending; nerve fiber transection; neurotransmitter release; newborn; nonhuman; priority journal; rat; reverse transcription polymerase chain reaction; Animals; Animals, Newborn; Brain Stem; Cadmium; Calcium Channel Blockers; Calcium Channels; Calcium Channels, L-Type; Dihydropyridines; DNA, Complementary; Facial Nerve; Ion Channel Gating; Motor Neurons; Nickel; Nitrendipine; omega-Agatoxin IVA; omega-Conotoxin GVIA; omega-Conotoxins; Patch-Clamp Techniques; Peptides; Rats; Rats, Wistar; Receptors, Cholinergic; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Spider Venoms
Año:1998
Volumen:18
Número:23
Página de inicio:9573
Página de fin:9584
Título revista:Journal of Neuroscience
Título revista abreviado:J. Neurosci.
ISSN:02706474
CODEN:JNRSD
CAS:Cadmium, 7440-43-9; Calcium Channel Blockers; Calcium Channels; Calcium Channels, L-Type; Dihydropyridines; DNA, Complementary; Nickel, 7440-02-0; Nitrendipine, 39562-70-4; omega-Agatoxin IVA; omega-Conotoxin GVIA, 92078-76-7; omega-conotoxin-MVIIC, 147794-23-8; omega-Conotoxins; Peptides; Receptors, Cholinergic; RNA, Messenger; Spider Venoms
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v18_n23_p9573_Plant

Referencias:

  • Bargas, J., Howe, A., Eberwine, J., Cao, Y., Surmeier, D.J., Cellular and molecular characterization of Ca2+ currents in acutely isolated, adult rat neostriatal neurons (1994) J Neurosci, 14, pp. 6667-6686
  • Catterall, W.A., Structure and function of voltage-gated ion channels (1995) Annu Rev Biochem, 64, pp. 493-531
  • Chin, H., Krall, M., Kim, H.-L., Kozak, C.A., Mock, B., The gene for the a, subunit of the skeletal muscle dihydropyridine-sensitive calcium channel (Cchl1a3) maps to mouse chromosome 1 (1992) Genomics, 14, pp. 1089-1091
  • Dunlap, K., Luebke, J.I., Turner, T.J., Exocytotic Ca2+ channels in mammalian central neurons (1995) Trends Neurosci, 18, pp. 89-98
  • Edwards, F.A., Konnerth, A., Sakmann, B., Takahashi, T., A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system (1989) Pflügers Arch, 414, pp. 600-612
  • Eliot, L.S., Johnston, D., Multiple components of calcium current in acutely dissociated dentate gyrus granule neurons (1994) J Neurophysiol, 72, pp. 762-777
  • Hillyard, D.R., Monje, V.D., Mintz, I.M., Bean, B.P., Nadasdi, L., Ramachandran, J., Miljanich, G., Olivera, B.M., A new Conus peptide ligand for mammalian presynaptic Ca2+ channels (1992) Neuron, 9, pp. 69-77
  • Hivert, B., Bouhanna, S., Diochot, S., Camu, W., Dayanithi, G., Henderson, C.E., Valmier, J., Embryonic rat motoneurons express a functional P-type voltage-dependent calcium channel (1995) Int J Dev Neurosci, 13, pp. 429-436
  • Hofmann, F., Biel, M., Flockerzi, V., Molecular basis for Ca2+ channel diversity (1994) Annu Rev Neurosci, 17, pp. 399-418
  • Huguenard, J.R., Low-threshold calcium currents in central nervous system neurons (1996) Annu Rev Physiol, 58, pp. 329-348
  • Katz, E., Ferro, P.A., Weisz, G., Uchitel, O.D., Calcium channels involved in synaptic transmission at the mature and regenerating mouse neuromuscular junction (1996) J Physiol, 497, pp. 687-697. , Lond
  • Katz, E., Protti, D.A., Ferro, P.A., Siri, M.D.R., Uchitel, O.D., Effects of Ca2+ channel blocker neurotoxins on transmitter release and presynaptic currents at the mouse neuromuscular junction (1997) Br J Pharmacol, 121, pp. 1531-1540
  • Lambolez, B., Audinat, E., Bochet, P., Crépel, F., Rossier, J., AMPA receptor subunits expressed by single Purkinje cells (1992) Neuron, 9, pp. 247-258
  • Llinás, R., Sugimori, M., Hillman, D.E., Cherskey, B., Distribution and functional significance of the P-type, voltage-dependent Ca2+ channels in the mammalian central nervous system (1992) Trends Neurosci, 15, pp. 351-355
  • Llinás, R., Sugimori, M., Cherksey, B.D., Smith, R.G., Delbono, O., Stefani, E., Appel, S., IgG from amyotrophic lateral sclerosis patients increases current through P-type calcium channels in mammalian cerebellar Purkinje cells and in isolated channel protein in lipid bilayer (1993) Proc Natl Acad Sci USA, 90, pp. 11743-11747
  • Ludwig, A., Flockerzi, V., Hofmann, F., Regional expression and cellular localization of the α1 and β subunit of high voltage-activated calcium channels in rat brain (1997) J Neurosci, 17, pp. 1339-1349
  • Magnelli, V., Baldelli, P., Carbone, E., Antagonists-resistant calcium currents in rat embryo motoneurons (1998) Eur J Neurosci, 10, pp. 1810-1825
  • McDonough, S.I., Swartz, K.J., Mintz, I.M., Boland, L.M., Bean, B.P., Inhibition of calcium channels in rat central and peripheral neurons by omega-conotoxin MVIIC (1996) J Neurosci, 16, pp. 2612-2623
  • Mintz, I.M., Adams, M.E., Bean, B.P., P-type calcium channels in rat central and peripheral neurons (1992) Neuron, 9, pp. 85-95
  • Mogul, D.J., Fox, A.P., Evidence for multiple types of Ca2+ channels in acutely isolated hippocampal CA3 neurons of the guinea pig (1991) J Physiol, 433, pp. 259-281. , Lond
  • Moreno, H., Rudy, B., Llinás, R., β subunits influence the biophysical and pharmacological differences between P- and Q-type calcium currents expressed in a mammalian cell line (1997) Proc Natl Acad Sci USA, 94, pp. 14042-14047
  • Mosier, D.R., Baldelli, P., Delbono, O., Smith, R.G., Alexianu, M.E., Appel, S.H., Stefani, E., Amyotrophic lateral sclerosis immunoglobulins increase Ca2+ currents in a motoneuron cell line (1995) Ann Neurol, 37, pp. 102-109
  • Mynlieff, M., Beam, K.G., Characterization of voltage-dependent calcium currents in mouse motoneurons (1992) J Neurophysiol, 68, pp. 85-92
  • Mynlieff, M., Beam, K.G., Adenosine acting at an A1 receptor decreases N-type calcium current in mouse motoneurons (1994) J Neurosci, 14, pp. 3628-3634
  • Olivera, B.M., McIntosh, J.M., Cruz, L.J., Luque, F.A., Gray, W.R., Purification and sequence of a presynaptic peptide toxin from Conus geographus venom (1984) Biochemistry, 23, pp. 5087-5090
  • Olivera, B.M., Miljanich, G.P., Ramachandran, J., Adams, M.E., Calcium channel diversity and neurotransmitter release: The omega-conotoxins and omega-agatoxins (1994) Annu Rev Biochem, 63, pp. 823-867
  • Ousley, A.H., Froehner, S.C., An anti-peptide antibody specific for the class a calcium channel α1 subunit labels mammalian neuromuscular junction (1994) Proc Natl Acad Sci USA, 91, pp. 12263-12267
  • Parent, L., Schneider, T., Moore, C.P., Talwar, D., Subunit regulation of the human brain α1E calcium channel (1997) J Membr Biol, 160, pp. 127-140
  • Perez-Reyes, E., Cribbs, L.L., Daud, A., Lacerda, A.E., Barclay, J., Williamson, M.P., Fox, M., Lee, J.-H., Molecular characterization of a neuronal low-voltage-activated T-type calcium channel (1998) Nature, 391, pp. 898-900
  • Plant, T., Schirra, C., Garaschuk, O., Rossier, J., Konnerth, A., Molecular determinants of NMDA receptor function in GABAergic neurones of rat forebrain (1997) J Physiol, 499, pp. 47-63. , Lond
  • Protti, D.A., Uchitel, O.D., Transmitter release and presynaptic Ca2+ currents blocked by the spider toxin omega-Aga-IVA (1993) NeuroReport, 5, pp. 333-336
  • Protti, D.A., Uchitel, O.D., P/Q-type calcium channels activate neighboring calcium-dependent potassium channels in mouse motor nerve terminals (1997) Pflügers Arch, 434, pp. 406-412
  • Randall, A., Tsien, R.W., Pharmacological dissection of multiple types of Ca2+ channel currents in rat cerebellar granule neurons (1995) J Neurosci, 15, pp. 2995-3012
  • Regan, L.J., Sah, D.W., Bean, B.P., Ca2+ channels in rat central and peripheral neurons: High threshold current resistant to dihydropyridine blockers and ω-conotoxin (1991) Neuron, 6, pp. 269-280
  • Stocker, J.W., Nadasdi, L., Aldrich, R.W., Tsien, R.W., Preferential interaction of omega-conotoxins with inactivated N-type Ca2+ channels (1997) J Neurosci, 17, pp. 3002-3013
  • Stuart, G.J., Dodt, H.-U., Sakmann, B., Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy (1993) Pflügers Arch, 423, pp. 511-518
  • Tanaka, O., Sakagami, H., Kondo, H., Localization of mRNAs of voltage-dependent Ca2+-channels: Four subtypes of α1- And β-subunits in developing and mature rat brain (1995) Mol Brain Res, 30, pp. 1-16
  • Tareilus, E., Roux, M., Qin, N., Olcese, R., Zhou, J.M., Stefani, E., Birnbaumer, L., A Xenopus oocyte β subunit: Evidence for a role in the assembly/expression of voltage-gated calcium channels that is separate from its role as a regulatory subunit (1997) Proc Natl Acad Sci USA, 94, pp. 1703-1708
  • Uchitel, O.D., Appel, S.H., Crawford, F., Sczcupak, L., Immunoglobulins from amyotrophic lateral sclerosis patients enhance spontaneous transmitter release from motor-nerve terminals (1988) Proc Natl Acad Sci USA, 85, pp. 7371-7374
  • Uchitel, O.D., Protti, D.A., Sanchez, V., Cherksey, B.D., Sugimori, M., Llinás, R., P-type voltage-dependent calcium channel mediates presynaptic calcium influx and transmitter release in mammalian synapses (1992) Proc Natl Acad Sci USA, 89, pp. 3330-3333
  • Umemiya, M., Berger, A.J., Properties and function of low- And high-voltage-activated Ca2+ channels in hypoglossal motoneurons (1994) J Neurosci, 14, pp. 5652-5660
  • Umemiya, M., Berger, A.J., Single-channel properties of four calcium channel types in rat motoneurons (1995) J Neurosci, 15, pp. 2218-2224
  • Umemiya, M., Araki, I., Kuno, M., Electrophysiological properties of axotomized facial motoneurones that are destined to die in neonatal rats (1993) J Physiol, 462, pp. 661-678. , Lond
  • Viana, F., Bayliss, D., Berger, A.J., Calcium conductances and their role in the firing behavior of neonatal rat hypoglossal motoneurons (1993) J Neurophysiol, 69, pp. 2137-2149
  • Viana, F., Van Den Bosch, L., Missiaen, L., Vandenberghe, W., Droogmans, G., Nilius, B., Robberecht, W., Mibefradil (Ro 40-5967) blocks multiple types of voltage-gated calcium channels in cultured rat spinal motoneurones (1997) Cell Calcium, 22, pp. 299-311
  • Westenbroek, R.E., Hoskins, L., Catterall, W.A., Localization of Ca2+ channel subtypes on rat spinal motoneurons, interneurons, and nerve terminals (1998) J Neurosci, 18, pp. 6319-6330
  • Wheeler, D.B., Randall, A., Sather, W.A., Tsien, R.W., Neuronal calcium channels encoded by the α1A subunit and their contribution to excitatory synaptic transmission in the CNS (1995) Gene Expression in the Central Nervous System, pp. 65-76. , (Yu ACH, Eng LF, McMahan KJ, Schulman H, Shooter EM, Stadlin A, eds), Amsterdam: Elsevier
  • Yan, Z., Surmeier, D.J., Muscarinic (m2/m4) receptors reduce N-and P-type Ca2+ currents in rat neostriatal cholinergic interneurons through a fast, membrane-delimited, G-protein pathway (1996) J Neurosci, 16, pp. 2592-2604

Citas:

---------- APA ----------
Plant, T.D., Schirra, C., Katz, E., Uchitel, O.D. & Konnerth, A. (1998) . Single-cell RT-PCR and functional characterization of Ca2+ channels in motoneurons of the rat facial nucleus. Journal of Neuroscience, 18(23), 9573-9584.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v18_n23_p9573_Plant [ ]
---------- CHICAGO ----------
Plant, T.D., Schirra, C., Katz, E., Uchitel, O.D., Konnerth, A. "Single-cell RT-PCR and functional characterization of Ca2+ channels in motoneurons of the rat facial nucleus" . Journal of Neuroscience 18, no. 23 (1998) : 9573-9584.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v18_n23_p9573_Plant [ ]
---------- MLA ----------
Plant, T.D., Schirra, C., Katz, E., Uchitel, O.D., Konnerth, A. "Single-cell RT-PCR and functional characterization of Ca2+ channels in motoneurons of the rat facial nucleus" . Journal of Neuroscience, vol. 18, no. 23, 1998, pp. 9573-9584.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v18_n23_p9573_Plant [ ]
---------- VANCOUVER ----------
Plant, T.D., Schirra, C., Katz, E., Uchitel, O.D., Konnerth, A. Single-cell RT-PCR and functional characterization of Ca2+ channels in motoneurons of the rat facial nucleus. J. Neurosci. 1998;18(23):9573-9584.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v18_n23_p9573_Plant [ ]