Artículo

Rocchetta, I.; Mazzuca, M.; Conforti, V.; Ruiz, L.; Balzaretti, V.; De Molina, M.D.C.R. "Effect of chromium on the fatty acid composition of two strains of Euglena gracilis" (2006) Environmental Pollution. 141(2):353-358
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Abstract:

The effect of hexavalent chromium on fatty acid composition was studied in two strains of Euglena gracilis; UTEX 753 (from the Culture Collection of Algae of Texas University, USA) and MAT (isolated from a highly polluted River). Both were grown in photoauxotrophic and photoheterotrophic conditions and exposed to two metal concentrations, one below and one above IC50. The high malondialdehyde (MDA) levels (3 to 7-fold) obtained with chromium concentration above IC50, suggested the existence of metal-induced lipid peroxidation. Total lipid content increased only with concentration below IC50, whereas it was inhibited by higher metal concentration. Photoheterotrophic control strains exhibited a significantly higher proportion of saturated and polyunsaturated fatty acids. Polyunsaturated acids were most affected by chromium, especially those related to chloroplast structures. Ultra-structure studies showed clear thylakoid disorganization in all treated cells. The results indicate that hexavalent chromium affects levels of fatty acids, especially those related to photosynthetic activity. © 2005 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Effect of chromium on the fatty acid composition of two strains of Euglena gracilis
Autor:Rocchetta, I.; Mazzuca, M.; Conforti, V.; Ruiz, L.; Balzaretti, V.; De Molina, M.D.C.R.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Departamento de Química, Facultad de Ciencias Naturales, Universidad de Patagonia, Comodoro Rivadavia, Chubut, Argentina
Palabras clave:Euglena gracilis; Fatty acids; Heavy metal; Hexavalent chromium; Lipids; Euglena gracilis; Hexavalent chromium; Malondialdehyde; MAT; Photoheterotrophic; Algae; Cells; Chlorophyll; Chromium; Fatty acids; Heavy metals; Lipids; Metals; Oxidation; Peroxides; Photosynthesis; Water pollution; Microorganisms; chromium; polyunsaturated fatty acid; chromium; fatty acid; article; auxotrophy; chloroplast; controlled study; Euglena gracilis; heterotrophy; IC 50; lipid analysis; lipid composition; lipid peroxidation; nonhuman; thylakoid; transmission electron microscopy; ultrastructure; Animals; Carcinogens, Environmental; Chloroplasts; Chromium; Culture Media; Euglena gracilis; Fatty Acids; Lipids; Malondialdehyde; Microscopy, Electron; Rivers; Water Pollutants, Chemical; algae; Euglena gracilis
Año:2006
Volumen:141
Número:2
Página de inicio:353
Página de fin:358
DOI: http://dx.doi.org/10.1016/j.envpol.2005.08.035
Título revista:Environmental Pollution
Título revista abreviado:Environ. Pollut.
ISSN:02697491
CODEN:ENPOE
CAS:chromium, 14092-98-9, 16065-83-1, 7440-47-3; Carcinogens, Environmental; Chromium, 7440-47-3; Culture Media; Fatty Acids; Lipids; Malondialdehyde, 542-78-9; Water Pollutants, Chemical; chromium hexavalent ion, 18540-29-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02697491_v141_n2_p353_Rocchetta

Referencias:

  • Bagchi, D., Stohs, S.J., Downs, B.W., Bagchi, M., Preuss, H.G., Cytotoxicity and oxidative mechanisms of different forms of chromium (2002) Toxicology, 180, pp. 5-22
  • Barsanti, L., Bastianini, A., Passarelli, V., Tredici, M.R., Gualtieri, P., Fatty acid content in wild type and WZSL mutant of Euglena gracilis (2000) J. Appl. Phycol., 12, pp. 515-520
  • Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Can. J. Biochem. Physiol., 37, pp. 911-917
  • Buetow, D.E., (1982) The Biology of Euglena, 3. , Academic Press New York
  • Buetow, D.E., (1989) Subcellular Biochemistry and Molecular Biology. the Biology of Euglena, 4. , Academic Press New York
  • Cervantes, C., Campos-García, J., Devars, S., Gutiérrez-Corona, F., Loza-Tavera, H., Torres-Guzmán, J.C., Moreno-Sánchez, R., Interactions of chromium with microorganisms and plants (2001) FEMS Microbiol. Rev., 25, pp. 335-347
  • Chini Zittelli, G., Lavista, F., Bastianini, A., Rodolfi, L., Vincenzini, M., Tredici, M.R., Production of eicosapentaenoic acid (EPA) by Nannochloropsis sp. Cultures in outdoor tubular photobioreactors (1999) J. Biotechnol., 70, pp. 299-312
  • Cieslak-Golonka, M., Toxic and Mutagenic Effects of Chromium (VI) (1996) Polyhedron Rep, 61. , Elsevier Science Ltd
  • Coppellotti, O., Glutathione, cysteine and acid-soluble thiol levels in Euglena gracilis cells exposed to copper an cadmium (1989) Comp. Biochem. Physiol., 94, pp. 35-40
  • Devars, S., Hernandez, R., Moreno-Sánchez, R., Enhanced heavy metal tolerance in two strains of photosynthetic Euglena gracilis by preexposure to mercury or cadmium. Arch. Environ. Contam (1998) Toxicol., 34, pp. 128-135
  • Duret, S., Bonaly, J., Bariaud, A., Vannereau, A., Mestre, J.C., Cadmium-induced ultrastructural changes in Euglena gracilis cells (1986) Environ. Res., 39, pp. 96-103
  • Einicker-Lamas, M., Soares, M.J., Soares, M.S., Olivera, M.M., Effects of cadmium on Euglena gracilis membrane lipids. Brazilian J (1996) Med. Biolog. Res., 29, pp. 941-948
  • Einicker-Lamas, M., Antunes Mezian, G., Benavides Fernandes, T., Silva, F.L.S., Guerra, F., Miranda, K., Attias, M., Oliveira, M.M., Euglena gracilis as a model for study of Cu2+ and Zn 2+ toxicity and accumulation in eukaryotic cells (2002) Environ. Pollut., 120, pp. 779-786
  • Girotti, A.W., Photosensitized oxidation of membrane lipids: Reaction pathways, cytotoxic effects, and cytoprotective mechanisms (2001) J. Photochem. Photobiol., 63, pp. 103-113
  • Haglund, K., The use of algae in aquatic toxicity assessment (1997) Progress in Phycological Research, 12. , F.E. Round D.J. Chapman Biopress Ltd
  • Harwood, J.L., Fatty acid metabolism. Ann. Rev. Plant Physiol (1988) Plant Mol. Biol., 39, pp. 101-138
  • Harwood, J.L., (1995) Plant Lipid Metabolism, p. 361. , J.C. Kader P. Mazliak Kluwer Acad. Publ., Dordrecht The Netherlands
  • Hodges, D.M., Delong, J.M., Forney, C.F., Prange, R.K., Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and others interfering compounds (1999) Planta, 207, pp. 604-611
  • Lepage, G., Roy, C., Improved recovery of fatty acid through direct transesterification without prior extraction or purification (1984) J. Lipid Res., 25, pp. 1391-1396
  • Molina Grima, E., Garcia Camacho, F., Sanchez Perez, J.A., Urda Cardona, J., Acien Fernandez Sevilla, J.M., Outdoor chemostat culture of Phaeodactylum tricornutum UTEX 640 in a tubular photobioreactor for the production of eicosapentaenoic acid (1994) Biotechnol. Appl. Biochem., 20, pp. 279-290
  • Mendoza-Cozatl, D., Devars, S., Loza-Tavera, H., Moreno-Sanchez, R., Cadmium accumulation in the choroplast of Euglena gracilis (2002) Physiol. Plant., 115, pp. 276-283
  • Navarro, L., Torres-Marquez, M.E., González-Moreno, S., Devars, S., Hernández, R., Moreno-Sánchez, R., Comparison of physiological changes in Euglena gracilis during exposure to heavy metals of heterotrophic and autotrophic cells (1997) Comp. Biochem. Physiol., 116 (3), pp. 265-272
  • Okamoto, O.K., Pinto, E., Latorre, L.R., Bechara, E.J.H., Colepicolo, P., Antioxidant modulation in response to metal-induced oxidative stress in algal chloroplasts (2001) Arch. Environ. Contam. Toxicol., 40, pp. 18-24
  • Rai, P.K., Rai, L.C., Algal response to heavy metals with special reference to mechanism of toxicity and tolerance (1998) Advances in Phycology, pp. 251-280. , B.N. Verma A.N. Kargupta S.K. Goyal APC Publications Pvt. Ltd. New Delhi
  • Ramalho, J.C., Campos, P.C., Teixeira, M., Nunes, M.A., Nitrogen dependent changes in antioxidant system and in fatty acid composition of chloroplast membranes from Coffea arabica L. plants submitted to high irradiance (1998) Plant. Sci., 135, pp. 115-124
  • Regnault, A., Chervin, D., Chammal, A., Piton, F., Calvayrac, R., Mazliak, P., Lipid composition of Euglena gracilis in relation to carbon-nitrogen balance (1995) Phytochemistry, 40, pp. 725-733
  • Rocchetta, I., Ruiz, L.B., Magaz, G., Conforti, V., Effects of hexavalent chromium in two strains of Euglena gracilis. Bull. Environ. Contam (2003) Toxicol., 70, pp. 1045-1051
  • Ruiz, L.B., Rocchetta, I., Dos Santos Ferreira, V., Conforti, V.T.D., Isolation, culture and characterization of a new strain of Euglena gracilis. New strain of Euglena gracilis (2004) Phycol. Res., 52 (2), pp. 168-174
  • (1985) Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms EPA/600/4-85/014/, pp. 76-103
  • Vajpayee, P., Rai, U.N., Ali, M.B., Tripathi, V., Yadav, V., Sinha, S., Singh, S.N., Chromium-induced physiologic changes in Vallisneria spiralis L. and its role in phytoremediation of tannery effluent (2001) Bull. Environ. Contam. Toxicol., 67, pp. 246-256
  • Vavilin, D.V., Ducruet, J.M., Matorin, D.N., Venediktov, P.S., Rubin, A.B., Membrane lipid peroxidation, cell viability and photosystem II activity in the green alga Chlorella pyrenoidosa subjected to various stress conditions (1998) J. Photochem. Photobiol. B Biol., 42, pp. 233-239
  • Wang, H., Clastogenicity of chromium contaminated soil samples evaluated by Vicia root-micronucleus assay (1999) Mutat. Res., 426, pp. 147-149
  • Watanabe, M., Suzuki, T., Involvement of reactive oxygen stress in damium-induced cellular damage in Euglena gracilis (2002) Comp. Biochem. Physiol. Part C, 131, pp. 491-500
  • Watanabe, M., Henmi, K., Ogawa, K., Suzuki, T., Cadmium-dependent generation of reactive oxygen species and mitochondrial DNA breaks in photosynthetic and non-photosynthetic strains of Euglena gracilis (2003) Comp. Biochem. Physiol. Part C, 134, pp. 227-234

Citas:

---------- APA ----------
Rocchetta, I., Mazzuca, M., Conforti, V., Ruiz, L., Balzaretti, V. & De Molina, M.D.C.R. (2006) . Effect of chromium on the fatty acid composition of two strains of Euglena gracilis. Environmental Pollution, 141(2), 353-358.
http://dx.doi.org/10.1016/j.envpol.2005.08.035
---------- CHICAGO ----------
Rocchetta, I., Mazzuca, M., Conforti, V., Ruiz, L., Balzaretti, V., De Molina, M.D.C.R. "Effect of chromium on the fatty acid composition of two strains of Euglena gracilis" . Environmental Pollution 141, no. 2 (2006) : 353-358.
http://dx.doi.org/10.1016/j.envpol.2005.08.035
---------- MLA ----------
Rocchetta, I., Mazzuca, M., Conforti, V., Ruiz, L., Balzaretti, V., De Molina, M.D.C.R. "Effect of chromium on the fatty acid composition of two strains of Euglena gracilis" . Environmental Pollution, vol. 141, no. 2, 2006, pp. 353-358.
http://dx.doi.org/10.1016/j.envpol.2005.08.035
---------- VANCOUVER ----------
Rocchetta, I., Mazzuca, M., Conforti, V., Ruiz, L., Balzaretti, V., De Molina, M.D.C.R. Effect of chromium on the fatty acid composition of two strains of Euglena gracilis. Environ. Pollut. 2006;141(2):353-358.
http://dx.doi.org/10.1016/j.envpol.2005.08.035