Abstract:
Hydrophobic interaction chromatography was employed for the fractionation of the glycopeptide mixture obtained after autoproteolysis and delipidization treatment of normal human seminal plasma samples. This sequence of procedures led to the elimination of highly hydrophobic components and to the concentration of the carbohydrate‐rich fractions. A partial separation between the O‐ and N‐glycosidic‐linked oligopeptides was also achieved after the chromatographic step. Copyright © 1987 Heyden & Son Ltd.
Registro:
Documento: |
Artículo
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Título: | Hydrophobic interaction chromatography of autoproteolysis products of human seminal plasma |
Autor: | Mazzini, M.N.; Di Giacomo, M.A.; Cerezo, A.S. |
Filiación: | Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
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Palabras clave: | glycopeptide; oligopeptide; chromatography; human; human cell; methodology; seminal plasma; Amino Acids; Chromatography, High Pressure Liquid; Glycopeptides; Human; Hydrolysis; Male; Semen; Support, Non-U.S. Gov't |
Año: | 1987
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Volumen: | 2
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Número: | 4
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Página de inicio: | 152
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Página de fin: | 155
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DOI: |
http://dx.doi.org/10.1002/bmc.1130020405 |
Título revista: | Biomedical Chromatography
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Título revista abreviado: | Biomed. Chromatogr.
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ISSN: | 02693879
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CAS: | Amino Acids; Glycopeptides
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02693879_v2_n4_p152_Mazzini |
Referencias:
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- Tortorella, H., de Cerezo, J.M.S., Mazzini, M.N., Cerezo, A.S., (1983) Am. J. Reprod. Immunol., 4, pp. 76-82
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Citas:
---------- APA ----------
Mazzini, M.N., Di Giacomo, M.A. & Cerezo, A.S.
(1987)
. Hydrophobic interaction chromatography of autoproteolysis products of human seminal plasma. Biomedical Chromatography, 2(4), 152-155.
http://dx.doi.org/10.1002/bmc.1130020405---------- CHICAGO ----------
Mazzini, M.N., Di Giacomo, M.A., Cerezo, A.S.
"Hydrophobic interaction chromatography of autoproteolysis products of human seminal plasma"
. Biomedical Chromatography 2, no. 4
(1987) : 152-155.
http://dx.doi.org/10.1002/bmc.1130020405---------- MLA ----------
Mazzini, M.N., Di Giacomo, M.A., Cerezo, A.S.
"Hydrophobic interaction chromatography of autoproteolysis products of human seminal plasma"
. Biomedical Chromatography, vol. 2, no. 4, 1987, pp. 152-155.
http://dx.doi.org/10.1002/bmc.1130020405---------- VANCOUVER ----------
Mazzini, M.N., Di Giacomo, M.A., Cerezo, A.S. Hydrophobic interaction chromatography of autoproteolysis products of human seminal plasma. Biomed. Chromatogr. 1987;2(4):152-155.
http://dx.doi.org/10.1002/bmc.1130020405