Artículo
Llambias, E.B.C.; Luo, J. "Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry" (1996) Biomedical Chromatography. 10(4):155-160
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Abstract:
Methods for the analysis of phenformin and its metabolite by high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESIMS) are developed. The effects of pH, buffer concentration and proportion of organic modifier on the retention of the compounds in HPLC have been studied. The optimum condition was used for the separation and identification of phenformin and its metabolite in microsomal metabolism by HPLC-ESIMS. A simple CE method is also described for the separation of these compounds. Optimum incubation conditions and cofactor requirements for the formation of 4-hydroxyphenformin by microsomal preparations of rat liver were determined. A linear response in the formation of product was found with increasing concentrations of protein and up to 15 min incubation. High concentrations of phenformin inhibited its metabolite formation, and K(m) was 4 μM.
Registro:
| Documento: | Artículo |
| Título: | Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry |
| Autor: | Llambias, E.B.C.; Luo, J. |
| Filiación: | Departmento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina MRC Toxicology Unit, Hodgkin Building, University of Leicester, Lancaster Road, Leicester LE1 9HN, United Kingdom |
| Palabras clave: | phenformin; animal cell; article; capillary electrophoresis; concentration response; controlled study; drug metabolism; high performance liquid chromatography; incubation time; ionization; liver microsome; mass spectrometry; metabolic inhibition; metabolite; nonhuman; ph; rat; Acetates; Animals; Buffers; Chromatography, High Pressure Liquid; Electrophoresis, Capillary; Female; Hydrogen-Ion Concentration; Hypoglycemic Agents; Mass Spectrometry; Metformin; Methanol; Microsomes, Liver; Online Systems; Osmolar Concentration; Phenformin; Rats; Spectrophotometry, Ultraviolet; Time Factors; Animalia |
| Año: | 1996 |
| Volumen: | 10 |
| Número: | 4 |
| Página de inicio: | 155 |
| Página de fin: | 160 |
| DOI: | http://dx.doi.org/10.1002/(SICI)1099-0801(199607)10:4<155::AID-BMC577>3.0.CO;2-Z |
| Título revista: | Biomedical Chromatography |
| Título revista abreviado: | BIOMED. CHROMATOGR. |
| ISSN: | 02693879 |
| CODEN: | BICHE |
| CAS: | 4-hydroxyphenformin, 17426-94-7; Acetates; ammonium acetate, 631-61-8; Buffers; Hypoglycemic Agents; Metformin, 657-24-9; Methanol, 67-56-1; Phenformin, 114-86-3 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02693879_v10_n4_p155_Llambias |
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Citas:
---------- APA ----------
Llambias, E.B.C. & Luo, J. (1996) . Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry. Biomedical Chromatography, 10(4), 155-160.http://dx.doi.org/10.1002/(SICI)1099-0801(199607)10:4<155::AID-BMC577>3.0.CO;2-Z
---------- CHICAGO ----------
Llambias, E.B.C., Luo, J. "Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry" . Biomedical Chromatography 10, no. 4 (1996) : 155-160.http://dx.doi.org/10.1002/(SICI)1099-0801(199607)10:4<155::AID-BMC577>3.0.CO;2-Z
---------- MLA ----------
Llambias, E.B.C., Luo, J. "Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry" . Biomedical Chromatography, vol. 10, no. 4, 1996, pp. 155-160.http://dx.doi.org/10.1002/(SICI)1099-0801(199607)10:4<155::AID-BMC577>3.0.CO;2-Z
---------- VANCOUVER ----------
Llambias, E.B.C., Luo, J. Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry. BIOMED. CHROMATOGR. 1996;10(4):155-160.http://dx.doi.org/10.1002/(SICI)1099-0801(199607)10:4<155::AID-BMC577>3.0.CO;2-Z
