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Abstract:

In this work we have studied the effects of ALA-mediated photodynamic therapy (PDT) on resting and mitogen-activated murine splenic lymphocytes, evaluating its impact on cell viability. We have also characterised the stress response, measuring the levels of antioxidant enzymes. A 2 h exposure to ALA produced 50% lethality upon irradiation of activated cells with 2.1 J/cm2. The decrease in cell survival with increasing time exposure to ALA, correlated well with the higher porphyrin accumulation. In resting lymphocytes, in spite of the low amount of porphyrins formed during 2 h incubation with ALA, 40% of the cells died after irradiation, this response was not further increased when higher amounts of porphyrins were synthesised. Superoxide dismutase was impaired by light treatment independently of ALA exposure in activated lymphocytes and, to a lesser extent, in resting lymphocytes. PDT induced an antioxidant adaptive response in activated cells 19 h after irradiation, reflected as a net increase in GSHPx activity and a slight reversion of the catalase (CAT) activity already impaired by light treatment. PDT treatment of activated cells also produced a diminution in the GSH/GSSG ratio. Only activated cells are capable of developing an antioxidant adaptive response to PDT treatment.

Registro:

Documento: Artículo
Título:Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response
Autor:Casas, A.; Perotti, C.; Fukuda, H.; del, A.M.; Batlle, C.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias, FCEyN, University of Buenos Aires, CONICET Ciudad Universitaria, Capital Federal, Argentina
Palabras clave:5-Aminolaevulinic acid; Catalase; Glutathione peroxidase; Lymphocytes; PDT; Photodynamic therapy; Superoxide dismutase
Año:2002
Volumen:17
Número:1
Página de inicio:42
Página de fin:50
DOI: http://dx.doi.org/10.1007/s10103-002-8265-6
Título revista:Lasers in Medical Science
Título revista abreviado:Lasers Med. Sci.
ISSN:02688921
CODEN:LMSCE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02688921_v17_n1_p42_Casas

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Citas:

---------- APA ----------
Casas, A., Perotti, C., Fukuda, H., del, A.M. & Batlle, C. (2002) . Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response. Lasers in Medical Science, 17(1), 42-50.
http://dx.doi.org/10.1007/s10103-002-8265-6
---------- CHICAGO ----------
Casas, A., Perotti, C., Fukuda, H., del, A.M., Batlle, C. "Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response" . Lasers in Medical Science 17, no. 1 (2002) : 42-50.
http://dx.doi.org/10.1007/s10103-002-8265-6
---------- MLA ----------
Casas, A., Perotti, C., Fukuda, H., del, A.M., Batlle, C. "Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response" . Lasers in Medical Science, vol. 17, no. 1, 2002, pp. 42-50.
http://dx.doi.org/10.1007/s10103-002-8265-6
---------- VANCOUVER ----------
Casas, A., Perotti, C., Fukuda, H., del, A.M., Batlle, C. Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response. Lasers Med. Sci. 2002;17(1):42-50.
http://dx.doi.org/10.1007/s10103-002-8265-6