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Abstract:

Fomes sclerodermeus was grown on semi-defined media based on yeast extract, peptone and glucose (YPG). The fungus produced a minimum basal level of laccase activity irrespective of culture medium. The highest laccase production (20 U cm-3) was obtained in cultures supplemented with CuSO4. Manganese peroxidase (MnP) could only be detected when MnSO4 was added to the medium. None of the aromatic compounds tested stimulated further laccase or MnP production. Laccase and MnP stimulated by Cu2+ or Mn2+ respectively were purified. Two different laccase isoenzymes with the same molecular mass (67 kDa) and N-linked carbohydrate content (30%) and a slight difference in their pI values (3.41 and 3.48) were characterized. In addition, two different MnP isoenzymes with the same molecular mass (47 kDa) and N-linked carbohydrate content (4%) and different pI values (3.35 and 3.45) were characterized. Both enzymes showed good stability at 25°C and over a wide range of pH. Both laccases oxidize ABTS (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) more efficiently than 2,6-dimethoxyphenol (DMP) with similar efficiency values (Kcat/Km) while the MnP 1, the major peroxidase isoenzyme in the studied conditions, oxidizes the Mn2+ and Mn-mediated activity on DMP more efficiently than MnP II. © 2006 Society of Chemical Industry.

Registro:

Documento: Artículo
Título:Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus Fomes sclerodermeus
Autor:Papinutti, L.; Martínez, M.J.
Filiación:Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Department of Molecular Microbiology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, E-28040 Madrid, Spain
Palabras clave:Aromatic compounds; Fomes sclerodermeus; Laccase; Manganese peroxidase; Purification; Aromatic compounds; Carbohydrates; Characterization; Enzymes; Glucose; Lignin; pH; Proteins; Purification; Fomes sclerodermeus; Laccase; Manganese peroxidase; Fungi; 2,2' azino bis(3 ethylbenzthiazoline 6 sulfonic acid); aromatic compound; carbohydrate; copper sulfate; glucose; isoenzyme; laccase; lignin; manganese peroxidase; manganese sulfate; peptone; phenol derivative; syringol; thiazole derivative; unclassified drug; Aromatic compounds; Carbohydrates; Characterization; Enzymes; Fungi; Glucose; Lignin; pH; Proteins; Purification; article; carbohydrate analysis; controlled study; culture medium; enzyme activity; enzyme analysis; enzyme metabolism; enzyme purification; enzyme stability; enzyme synthesis; Fomes sclerodermeus; fungus; molecular weight; nonhuman; pH measurement; temperature measurement; Fomes; Fungi
Año:2006
Volumen:81
Número:6
Página de inicio:1064
Página de fin:1070
DOI: http://dx.doi.org/10.1002/jctb.1537
Título revista:Journal of Chemical Technology and Biotechnology
Título revista abreviado:J. Chem. Technol. Biotechnol.
ISSN:02682575
CODEN:JCTBD
CAS:copper sulfate, 7758-98-7, 7758-99-8; glucose, 50-99-7, 84778-64-3; laccase, 80498-15-3; lignin, 9005-53-2; manganese peroxidase, 114995-15-2; manganese sulfate, 10124-55-7, 7785-87-7; syringol, 91-10-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02682575_v81_n6_p1064_Papinutti

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Citas:

---------- APA ----------
Papinutti, L. & Martínez, M.J. (2006) . Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus Fomes sclerodermeus. Journal of Chemical Technology and Biotechnology, 81(6), 1064-1070.
http://dx.doi.org/10.1002/jctb.1537
---------- CHICAGO ----------
Papinutti, L., Martínez, M.J. "Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus Fomes sclerodermeus" . Journal of Chemical Technology and Biotechnology 81, no. 6 (2006) : 1064-1070.
http://dx.doi.org/10.1002/jctb.1537
---------- MLA ----------
Papinutti, L., Martínez, M.J. "Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus Fomes sclerodermeus" . Journal of Chemical Technology and Biotechnology, vol. 81, no. 6, 2006, pp. 1064-1070.
http://dx.doi.org/10.1002/jctb.1537
---------- VANCOUVER ----------
Papinutti, L., Martínez, M.J. Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus Fomes sclerodermeus. J. Chem. Technol. Biotechnol. 2006;81(6):1064-1070.
http://dx.doi.org/10.1002/jctb.1537