Abstract:
Bacillus amyloliquefaciens MIR‐41 produces three exocellular amylolytic enzymes: α‐amylase, pullulanase and α‐glucosidase. Physicochemical factors such as pH and/or temperature may affect the enzyme yield. Continuous culture at a dilution rate of 0.15 h−1 demonstrated that the maximum production of α‐amylase and α‐glucosidase occurred at 36°C and pH 6.8 and of pullulanase at 44°C and pH 5.6. The results indicated that combinations of temperature and pH might be used for the production of crude extracts of α‐amylase or pullulanase. Copyright © 1993 Society of Chemical Industry
Registro:
Documento: |
Artículo
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Título: | Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41 |
Autor: | Castro, G.R.; Baigorí, M.D.; Méndez, B.S.; Siñeriz, F. |
Filiación: | Facultad de Bioquímica, Universidad Nacional de Tucumán, Belgrano y Caseros, Avda, Tucumán, 4000, Argentina Laboratorio de Microbiología, Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, 1428, Argentina
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Palabras clave: | Bacillus amyloliquefaciens; continuous culture; enzyme production; pullulanase; α‐amylase; α‐glucosidase; alpha glucosidase; amylase; pullulanase; article; bacillus amyloliquefaciens; chemical analysis; continuous culture; enzyme synthesis; nonhuman; ph; temperature sensitivity |
Año: | 1993
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Volumen: | 58
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Número: | 3
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Página de inicio: | 277
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Página de fin: | 280
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DOI: |
http://dx.doi.org/10.1002/jctb.280580311 |
Título revista: | Journal of Chemical Technology & Biotechnology
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Título revista abreviado: | J. Chem. Technol. Biotechnol.
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ISSN: | 02682575
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CAS: | alpha glucosidase, 9001-42-7; amylase, 9000-90-2, 9000-92-4, 9001-19-8; pullulanase, 9075-68-7
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02682575_v58_n3_p277_Castro |
Referencias:
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- Castro, G.R., Ferrero, M.A., Abate, C.M., Méndez, B.S., Siñeriz, F., Simultaneous production of α‐ and β‐amylases by Bacillus subtilis MIR‐5 in batch and continuous culture (1992) Biotechnol. Lett., 14, pp. 49-54
- Castro, G.R., Méndez, B.S., Siñeriz, F., Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR‐41 in batch and continuous culture (1993) J. Chem. Tech. Biotechnol., 56, pp. 289-294
- Castro, G.R., (1991), Fermentative Studies in Species of the Genus Bacillus. PhD thesis, Univ. Buenos Aires, (in Spanish); Castro, G.R., Ferrero, M.A., Méndez, B.S., Siñeriz, F., A system for the differentiation of some closely related Bacillus spp (1991) J. Biotechnol., 20, pp. 105-108
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- Svodobodová, J., Julák, J., Pilar, J., Svodoboda, P., Membrane fluidity in Bacillus subtilis. Validity of homeoviscous adaptation (1988) Folia Microbiol., 33, pp. 170-177
- Schwartz, M., Hofnung, M., La maltodextrine phosphorylase d'Escherichia coli (1967) Eur. J. Biochem., 2, pp. 132-135
- Wallenfels, K., Bender, H., Rached, J.R., Pullulanase from Aerobacter aerogenes; production in cell bound state. Purification and properties of the enzyme (1966) Biochem. Biophys. Commun., 22, pp. 254-266
- Frankena, J., Koningtein, G.M., van Versveld, H.W., Stouthamer, A.H., Effect of different limitations in chemostat cultures on growth and production of extracellular protease by (1986) Bacillus Iicheniformis. Appl. Microbiol. Biotechnol., 24, pp. 106-112
Citas:
---------- APA ----------
Castro, G.R., Baigorí, M.D., Méndez, B.S. & Siñeriz, F.
(1993)
. Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41. Journal of Chemical Technology & Biotechnology, 58(3), 277-280.
http://dx.doi.org/10.1002/jctb.280580311---------- CHICAGO ----------
Castro, G.R., Baigorí, M.D., Méndez, B.S., Siñeriz, F.
"Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41"
. Journal of Chemical Technology & Biotechnology 58, no. 3
(1993) : 277-280.
http://dx.doi.org/10.1002/jctb.280580311---------- MLA ----------
Castro, G.R., Baigorí, M.D., Méndez, B.S., Siñeriz, F.
"Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41"
. Journal of Chemical Technology & Biotechnology, vol. 58, no. 3, 1993, pp. 277-280.
http://dx.doi.org/10.1002/jctb.280580311---------- VANCOUVER ----------
Castro, G.R., Baigorí, M.D., Méndez, B.S., Siñeriz, F. Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41. J. Chem. Technol. Biotechnol. 1993;58(3):277-280.
http://dx.doi.org/10.1002/jctb.280580311