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Abstract:

Bacillus amyloliquefaciens MIR‐41 produces three exocellular amylolytic enzymes: α‐amylase, pullulanase and α‐glucosidase. Physicochemical factors such as pH and/or temperature may affect the enzyme yield. Continuous culture at a dilution rate of 0.15 h−1 demonstrated that the maximum production of α‐amylase and α‐glucosidase occurred at 36°C and pH 6.8 and of pullulanase at 44°C and pH 5.6. The results indicated that combinations of temperature and pH might be used for the production of crude extracts of α‐amylase or pullulanase. Copyright © 1993 Society of Chemical Industry

Registro:

Documento: Artículo
Título:Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41
Autor:Castro, G.R.; Baigorí, M.D.; Méndez, B.S.; Siñeriz, F.
Filiación:Facultad de Bioquímica, Universidad Nacional de Tucumán, Belgrano y Caseros, Avda, Tucumán, 4000, Argentina
Laboratorio de Microbiología, Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:Bacillus amyloliquefaciens; continuous culture; enzyme production; pullulanase; α‐amylase; α‐glucosidase; alpha glucosidase; amylase; pullulanase; article; bacillus amyloliquefaciens; chemical analysis; continuous culture; enzyme synthesis; nonhuman; ph; temperature sensitivity
Año:1993
Volumen:58
Número:3
Página de inicio:277
Página de fin:280
DOI: http://dx.doi.org/10.1002/jctb.280580311
Título revista:Journal of Chemical Technology & Biotechnology
Título revista abreviado:J. Chem. Technol. Biotechnol.
ISSN:02682575
CAS:alpha glucosidase, 9001-42-7; amylase, 9000-90-2, 9000-92-4, 9001-19-8; pullulanase, 9075-68-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02682575_v58_n3_p277_Castro

Referencias:

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  • Luenser, S., Microbial enzymes for industrial sweetener production (1983) Develop. Ind. Microbiol., 24, pp. 76-96
  • Pirt, S.J., (1975) Principles of Microbe and Cell Cultivation, , Blackwell Scientific Publications, London
  • Dawes, I.W., Thornley, J.H.M., Sporulation in Bacillus subtilis. Theoretical and experimental studies in continuous culture system (1970) J. Gen. Microbiol., 62, pp. 49-66
  • Priest, F.G., Thirunavukkarasu, M., Synthesis and localization of α‐amylase and α‐glucosidase in Bacillus licheniformis grown in batch and continuous culture (1985) J. Appl. Bacteriol., 58, pp. 381-390
  • Castro, G.R., Ferrero, M.A., Abate, C.M., Méndez, B.S., Siñeriz, F., Simultaneous production of α‐ and β‐amylases by Bacillus subtilis MIR‐5 in batch and continuous culture (1992) Biotechnol. Lett., 14, pp. 49-54
  • Castro, G.R., Méndez, B.S., Siñeriz, F., Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR‐41 in batch and continuous culture (1993) J. Chem. Tech. Biotechnol., 56, pp. 289-294
  • Castro, G.R., (1991), Fermentative Studies in Species of the Genus Bacillus. PhD thesis, Univ. Buenos Aires, (in Spanish); Castro, G.R., Ferrero, M.A., Méndez, B.S., Siñeriz, F., A system for the differentiation of some closely related Bacillus spp (1991) J. Biotechnol., 20, pp. 105-108
  • Castro, G.R., Méndez, B.S., Siñeriz, F., Protein measurement with neocuproine reactive (1991) Biotechnol. Tech., 5, pp. 431-436
  • Svodobodová, J., Julák, J., Pilar, J., Svodoboda, P., Membrane fluidity in Bacillus subtilis. Validity of homeoviscous adaptation (1988) Folia Microbiol., 33, pp. 170-177
  • Schwartz, M., Hofnung, M., La maltodextrine phosphorylase d'Escherichia coli (1967) Eur. J. Biochem., 2, pp. 132-135
  • Wallenfels, K., Bender, H., Rached, J.R., Pullulanase from Aerobacter aerogenes; production in cell bound state. Purification and properties of the enzyme (1966) Biochem. Biophys. Commun., 22, pp. 254-266
  • Frankena, J., Koningtein, G.M., van Versveld, H.W., Stouthamer, A.H., Effect of different limitations in chemostat cultures on growth and production of extracellular protease by (1986) Bacillus Iicheniformis. Appl. Microbiol. Biotechnol., 24, pp. 106-112

Citas:

---------- APA ----------
Castro, G.R., Baigorí, M.D., Méndez, B.S. & Siñeriz, F. (1993) . Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41. Journal of Chemical Technology & Biotechnology, 58(3), 277-280.
http://dx.doi.org/10.1002/jctb.280580311
---------- CHICAGO ----------
Castro, G.R., Baigorí, M.D., Méndez, B.S., Siñeriz, F. "Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41" . Journal of Chemical Technology & Biotechnology 58, no. 3 (1993) : 277-280.
http://dx.doi.org/10.1002/jctb.280580311
---------- MLA ----------
Castro, G.R., Baigorí, M.D., Méndez, B.S., Siñeriz, F. "Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41" . Journal of Chemical Technology & Biotechnology, vol. 58, no. 3, 1993, pp. 277-280.
http://dx.doi.org/10.1002/jctb.280580311
---------- VANCOUVER ----------
Castro, G.R., Baigorí, M.D., Méndez, B.S., Siñeriz, F. Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41. J. Chem. Technol. Biotechnol. 1993;58(3):277-280.
http://dx.doi.org/10.1002/jctb.280580311