Abstract:
Bacillus amyloliquefaciens MIR‐41 has been isolated from natural sources and characterized using statistical methods. The strain showed three types of glucanase activities: α‐amylase, α‐glucosidase and pullulanase in defined medium and starch as carbon source. Extracellular activity of α‐amylase was approximately three times higher in continuous culture than in batch. Maximal productivity was found at a dilution rate of 0·16 h−1. Alpha‐glucosidase was expressed as a cell‐bound enzyme in batch culture, but in continuous culture was mostly extracellular. Pullulanase was cell‐bound in all cases. The expression of the enzymes was dependent upon the system and the dilution rate used. No cell lysis was detected in the chemostat as compared with batch cultures. Copyright © 1993 Society of Chemical Industry
Registro:
Documento: |
Artículo
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Título: | Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR‐41 in batch and continuous culture |
Autor: | Castro, G.R.; Méndez, B.S.; Siñeriz, F. |
Filiación: | Cátedra de Microbiología Superior, Facultad de Bioquímica, Universidad Nacional de Tucumán, Tucumán, Tucumán, 4000, Argentina Laboratorio de Microbiología, Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, 1428, Argentina
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Palabras clave: | Bacillus amyloliquefaciens; continuous culture; pullulanase; α‐amylase; α‐glucosidase; alpha glucosidase; amylase; pullulanase; starch; article; bacillus amyloliquefaciens; continuous culture; enzyme activity; enzyme synthesis; intermethod comparison; lysis; nonhuman |
Año: | 1993
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Volumen: | 56
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Número: | 3
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Página de inicio: | 289
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Página de fin: | 294
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DOI: |
http://dx.doi.org/10.1002/jctb.280560312 |
Título revista: | Journal of Chemical Technology & Biotechnology
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Título revista abreviado: | J. Chem. Technol. Biotechnol.
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ISSN: | 02682575
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CAS: | alpha glucosidase, 9001-42-7; amylase, 9000-90-2, 9000-92-4, 9001-19-8; pullulanase, 9075-68-7; starch, 9005-25-8, 9005-84-9
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02682575_v56_n3_p289_Castro |
Referencias:
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Citas:
---------- APA ----------
Castro, G.R., Méndez, B.S. & Siñeriz, F.
(1993)
. Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR‐41 in batch and continuous culture. Journal of Chemical Technology & Biotechnology, 56(3), 289-294.
http://dx.doi.org/10.1002/jctb.280560312---------- CHICAGO ----------
Castro, G.R., Méndez, B.S., Siñeriz, F.
"Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR‐41 in batch and continuous culture"
. Journal of Chemical Technology & Biotechnology 56, no. 3
(1993) : 289-294.
http://dx.doi.org/10.1002/jctb.280560312---------- MLA ----------
Castro, G.R., Méndez, B.S., Siñeriz, F.
"Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR‐41 in batch and continuous culture"
. Journal of Chemical Technology & Biotechnology, vol. 56, no. 3, 1993, pp. 289-294.
http://dx.doi.org/10.1002/jctb.280560312---------- VANCOUVER ----------
Castro, G.R., Méndez, B.S., Siñeriz, F. Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR‐41 in batch and continuous culture. J. Chem. Technol. Biotechnol. 1993;56(3):289-294.
http://dx.doi.org/10.1002/jctb.280560312