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Abstract:

Background: Human spermatozoa decondense in vitro upon exposure to heparin and glutathione. Glutathione is also the disulfide bond reducer in vivo, and heparan sulfate, a functional analogue of heparin, has been proposed as the protamine acceptor. The aim of this study was to evaluate the decondensing ability of chemically modified heparins and different glycosaminoglycans (GAGs) on isolated sperm nuclei in vitro, and to analyse the possible role of different GAGs as protamine acceptors. Methods: Capacitated spermatozoa and isolated sperm nuclei from normospermic semen samples were decondensed in the presence of heparin (or its equivalent) and glutathione. After fixation with glutaraldehyde, the percentage of decondensed spermatozoa and nuclei was determined under phase-contrast. Proteins were extracted from sperm nuclei previously incubated in the presence of gluhathione and different GAGs by incubation with urea-β-meracptoethanol-NaCl, and analysed by acid polyacrylamide gel electrophoresis. Results: The ability of desulfated heparins and other GAGs to decondense isolated nuclei mirrored exactly the decondensation of capacitated spermatozoa, the only difference being the level of maximum decondensation achieved. Heparan sulfate and heparin, but not other GAGs, were able to release protamines from sperm chromatin. Conclusions: Heparan sulfate could be functioning as protamine acceptor in vivo during human sperm nuclear decondensation. © The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.

Registro:

Documento: Artículo
Título:Further evidence on the role of heparan sulfate as protamine acceptor during the decondensation of human spermatozoa
Autor:Romanato, M.; Regueira, E.; Cameo, M.S.; Baldini, C.; Calvo, L.; Calvo, J.C.
Filiación:Biología de la Reproducción, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Department of Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Heparan sulfate; Protamine; Sperm nuclear decondensation; glutaraldehyde; glutathione; glycosaminoglycan; heparan sulfate; heparin; protamine; article; carbohydrate analysis; cell isolation; chromatin condensation; drug activity; human; human cell; immunocytochemistry; in vitro study; male; phase contrast microscopy; polyacrylamide gel electrophoresis; protein analysis; protein isolation; protein secretion; semen analysis; spermatozoon capacitation; spermatozoon density
Año:2005
Volumen:20
Número:10
Página de inicio:2784
Página de fin:2789
DOI: http://dx.doi.org/10.1093/humrep/dei124
Título revista:Human Reproduction
Título revista abreviado:Hum. Reprod.
ISSN:02681161
CODEN:HUREE
CAS:glutaraldehyde, 111-30-8, 37245-61-7; glutathione, 70-18-8; heparan sulfate, 9050-30-0; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; protamine, 11061-43-1, 9007-31-2, 9012-00-4
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_02681161_v20_n10_p2784_Romanato.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v20_n10_p2784_Romanato

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Citas:

---------- APA ----------
Romanato, M., Regueira, E., Cameo, M.S., Baldini, C., Calvo, L. & Calvo, J.C. (2005) . Further evidence on the role of heparan sulfate as protamine acceptor during the decondensation of human spermatozoa. Human Reproduction, 20(10), 2784-2789.
http://dx.doi.org/10.1093/humrep/dei124
---------- CHICAGO ----------
Romanato, M., Regueira, E., Cameo, M.S., Baldini, C., Calvo, L., Calvo, J.C. "Further evidence on the role of heparan sulfate as protamine acceptor during the decondensation of human spermatozoa" . Human Reproduction 20, no. 10 (2005) : 2784-2789.
http://dx.doi.org/10.1093/humrep/dei124
---------- MLA ----------
Romanato, M., Regueira, E., Cameo, M.S., Baldini, C., Calvo, L., Calvo, J.C. "Further evidence on the role of heparan sulfate as protamine acceptor during the decondensation of human spermatozoa" . Human Reproduction, vol. 20, no. 10, 2005, pp. 2784-2789.
http://dx.doi.org/10.1093/humrep/dei124
---------- VANCOUVER ----------
Romanato, M., Regueira, E., Cameo, M.S., Baldini, C., Calvo, L., Calvo, J.C. Further evidence on the role of heparan sulfate as protamine acceptor during the decondensation of human spermatozoa. Hum. Reprod. 2005;20(10):2784-2789.
http://dx.doi.org/10.1093/humrep/dei124