Artículo

Romanato, M.; Cameo, M.S.; Bertolesi, G.; Baldini, C.; Calvo, J.C.; Calvo, L. "Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo" (2003) Human Reproduction. 18(9):1868-1873
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Background: Human sperm decondense in vitro upon exposure to heparin and glutathione. The aim of this study was to evaluate whether this decondensing ability of heparin in vitro is related to structural characteristics of the molecule and to test the in-vitro decondensing ability of other glycosaminoglycans. Methods: Capacitated sperm obtained from normospermic semen samples were decondensed in the presence of heparin (or its equivalent) and glutathione. After fixation with glutaraldehyde, the percentage of decondensed sperm was determined under phase contrast. Results: The decondensing ability of heparin was related to sulphation characteristics of the molecule: heparin, O-desulphated heparin and N-desulphated-N-acetylated heparin had similar decondensing abilities; N-desulphated was less active and O/N-desulphated-N-acetylated heparin was completely inactive. On the other hand, the decondensing ability of heparin was not affected by molecular weight, within the range 3000-18 000 kDa. When the decondensing ability of different glycosaminoglycans was tested, heparin and heparin sulphate were equally active, while chondroitin sulphate and hyaluronic acid were completely inactive and dermatan sulphate was slightly active. Conclusions: Our results indicate that heparin's decondensing ability in vitro is related to sulphation characteristics of the molecule and suggest that heparan sulphate, a structural analogue of heparin, could be a sperm-decondensing agent in vivo.

Registro:

Documento: Artículo
Título:Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo
Autor:Romanato, M.; Cameo, M.S.; Bertolesi, G.; Baldini, C.; Calvo, J.C.; Calvo, L.
Filiación:Biologia de la Reproduccion, Fac. de Cie. Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Inst. de Biol. y Med. Experimental, Fac. de Cie. Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Biological Chemistry, Fac. de Cie. Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Fertilization; Gamete biology; Sperm; dermatan sulfate; glutaraldehyde; glutathione; glycosaminoglycan; heparan sulfate; heparin; heparin derivative; hyaluronic acid; article; controlled study; dose response; drug activity; drug effect; drug sulfation; human; human cell; in vitro study; in vivo study; male; molecular weight; normal human; phase contrast microscopy; sperm; spermatozoon; structure activity relation
Año:2003
Volumen:18
Número:9
Página de inicio:1868
Página de fin:1873
DOI: http://dx.doi.org/10.1093/humrep/deg354
Título revista:Human Reproduction
Título revista abreviado:Hum. Reprod.
ISSN:02681161
CODEN:HUREE
CAS:dermatan sulfate, 24967-94-0; glutaraldehyde, 111-30-8, 37245-61-7; glutathione, 70-18-8; heparan sulfate, 9050-30-0; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; hyaluronic acid, 31799-91-4, 9004-61-9, 9067-32-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v18_n9_p1868_Romanato

Referencias:

  • Ball, G.D., Bellin, M.E., Ax, R.L., First, N.L., Glycosaminoglycans in bovine cumulus-oocyte complexes: Morphology and chemistry (1982) Mol. Cell. Endocrinol., 28, pp. 113-122
  • Bedford, J.M., Bent, M., Calvin, H., Variations in the structural character and stability of the nuclear chromatin in morphologically normal human spermatozoa (1973) J. Reprod. Fertil., 33, pp. 19-29
  • Bellin, M., Ax, R., Laufer, N., Tarlatzis, B., DeCherney, A., Feldberg, D., Haseltine, F., Glycosaminoglycans in follicular fluid from women undergoing in vitro fertilization and their relationship to cumulus expansion, fertilization and development (1986) Fertil. Steril., 45, pp. 244-248
  • Bertolesi, G., Heparin and related glycosaminoglycans: Effects on tumoral development (1998), PhD Thesis. Faculty of Exact and Natural Sciences, University of Buenos Aires, Argentina; Carrell, D., Liu, L., Heparin binding sites are present at a higher concentration on sperm of subfertile men than donors of known fertility (2002) Arch. Androl., 48, pp. 147-154
  • Chargaff, D., Olson, K., Studies on the chemistry of blood coagulation. VI. Studies on the action of heparin and other coagulants. The influence of protamine on the anticoagulant effect in vivo (1938) J. Biol. Chem., 122, pp. 153-167
  • Delgado, N., Reyes, R., Huacuja, L., Merchant, H., Rosado, A., Heparin binding sites in the human spermatozoa membrane (1982) Arch. Androl., 8, pp. 87-95
  • Delgado, N.M., Reyes, R., Mora-Galindo, J., Rosado, A., Size-uniform fragments as nuclear decondensation and acrosome reaction inducers in human spermatozoa (1988) Life Sci., 42, pp. 2177-2183
  • Eriksen, G., Malmstöm, A., Uldbjerg, N., Human follicular fluid proteoglycans in relation to in vitro fertilization (1997) Fertil. Steril., 68, pp. 791-798
  • Fedarko, N., Conrad, E., A unique heparin sulfate in the nucleus of hepatocytes: Structural changes with the growth of the cells (1986) J. Cell Biol., 102, pp. 587-599
  • Gaubeca-Klix, E., Marin-Briggiler, C., Cameo, M., Calvo, L., In vitro sperm nuclear decondensation in the presence of heparin/glutathione distinguishes two subgroups of infertile patients not identified by conventional semen analysis (1998) Treatment of Infertility: The New Frontiers, , (Abstract book). Boca Raton, FL. Abstract PO-51
  • Gebauer, H., Linder, H., Amsterdam, A., Synthesis of heparin-like glycosaminoglycans in rat ovarian slices (1978) Biol. Reprod., 18, pp. 350-358
  • Grimek, N., Reyes, R., Gallind, J., Rosado, A., Characteristics of proteoglycans isolated from small and large bovine ovarian follicles (1988) Biol. Reprod., 30, pp. 397-409
  • Jackson, R., Busch, S., Cardin, A., Glycosaminoglycans: Molecular properties, protein interactions and role in physiological processes (1991) Physiol. Rev., 71, pp. 481-539
  • Kawasaki, K., Philpott, A., Avillion, A.A., Berrios, M., Fisher, P.A., Chromatin decondensation in Drosophila embryo extracts (1994) J. Biol. Chem., 269, pp. 10169-10176
  • Lalich, R.A., Vedantham, S., McCormick, N., Wagner, C., Prins, G.S., Relationship between heparin binding characteristics and ability of human spermatozoa to penetrate hamster ova (1989) J. Reprod. Fertil., 86, pp. 297-302
  • Lasalle, B., Testart, J., Relationship between fertilizing ability of frozen human spermatozoa and capacity for heparin binding and nuclear decondensation (1992) J. Reprod. Fertil., 95, pp. 313-324
  • Lee, C., Ax, R., Concentrations and composition of glycosaminoglycans in the female bovine follicular fluid (1984) Nature, 224, pp. 919-920
  • Lindahl, U., Kjellén, L., Heparin or heparan sulfate - What is the difference? (1991) Thromb. Haemostasis, 66, pp. 44-48
  • Maeda, Y., Yanagimachi, H., Tateno, H., Usui, N., Yanagimachi, R., Decondensation of the mouse sperm nucleus within the interphase nucleus (1998) Zygote, 6, pp. 39-45
  • Montag, M., Tok, V., Liow, S., Bongson, A., Nicolle, J.C., In vitro decondensation of mammalian sperm and subsequent formation of pronuclei-like structures for micromanipulation (1992) Mol. Reprod. Dev., 33, pp. 338-346
  • Ohsumi, K., Katagiri, C., Characterization of the ooplasmic factor inducing decondensation of and protamine removal from toad sperm nuclei: Involvement of nucleoplasmin (1991) Dev. Biol., 148, pp. 295-305
  • Perreault, S.D., Wolff, R.A., Zirkin, B.R., The role of disulfide bond reduction during mammalian sperm nuclear decondensation in vitro (1984) Dev. Biol., 101, pp. 160-167
  • Perreault, S.D., Barbu, R.R., Slott, V.L., Importance of glutathione in the acquisition and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes (1988) Dev. Biol., 125, pp. 181-186
  • Philpott, A., Leno, G.H., Laskey, R.A., Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin (1991) Cell, 65, pp. 569-578
  • Reyes, R., Rosado, A., Hernández, O., Delgado, N.M., Heparin and glutathione: Physiological decondensing agents of human sperm nuclei (1989) Gamete Res., 23, pp. 39-47
  • Romanato, M., Bertolesi, G., Calvo, J.C., Calvo, L., Heparan sulfate: A putative sperm decondensing agent for human spermatozoa in vivo (2001) J. Androl., 22 (SUPPL.), p. 85
  • Salustri, A., Yanagishita, M., Hascall, V.C., Synthesis and accumulation of hyaluronic acid and proteoglycans in the mouse cumulus cell-oocyte complex during follicle-stimulating hormone-induced mucification (1989) J. Biol. Chem., 264, pp. 13840-13847
  • Tesarik, J., Kopecny, V., Development of human male pronucleus: Ultrastructure and timing (1989) Gamete Res., 24, pp. 135-149
  • (1999) WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, 4th edn., , Cambridge University Press, Cambridge
  • Yanagimachi, R., Mammalian fertilization (1994) The Physiology of Reproduction, 2nd edn, pp. 189-317. , In Knobil, E. and Neill, J.D. (eds); Raven Press, New York
  • Yanagishita, M., Hascall, V.C., Cell surface heparan sulfate proteoglycans (1992) J. Biol. Chem., 267, pp. 9451-9454
  • Yanagishita, M., Rodbard, D., Hascall, V., Isolation and characterization of proteoglycans from porcine ovarian follicular fluid (1979) J. Biol. Chem., 254, pp. 911-920
  • Zirkin, B.R., Perreault, S.D., Naish, S.J., Formation and function of the male pronuceus during mammalian fertilization (1989) The Molecular Biology of Fertilization, pp. 91-114. , In Schatten, H. and Schatten, G. (eds); Academic Press, San Diego, CA

Citas:

---------- APA ----------
Romanato, M., Cameo, M.S., Bertolesi, G., Baldini, C., Calvo, J.C. & Calvo, L. (2003) . Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo. Human Reproduction, 18(9), 1868-1873.
http://dx.doi.org/10.1093/humrep/deg354
---------- CHICAGO ----------
Romanato, M., Cameo, M.S., Bertolesi, G., Baldini, C., Calvo, J.C., Calvo, L. "Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo" . Human Reproduction 18, no. 9 (2003) : 1868-1873.
http://dx.doi.org/10.1093/humrep/deg354
---------- MLA ----------
Romanato, M., Cameo, M.S., Bertolesi, G., Baldini, C., Calvo, J.C., Calvo, L. "Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo" . Human Reproduction, vol. 18, no. 9, 2003, pp. 1868-1873.
http://dx.doi.org/10.1093/humrep/deg354
---------- VANCOUVER ----------
Romanato, M., Cameo, M.S., Bertolesi, G., Baldini, C., Calvo, J.C., Calvo, L. Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo. Hum. Reprod. 2003;18(9):1868-1873.
http://dx.doi.org/10.1093/humrep/deg354